Construction of a Nitrogenase Model Using Metal Cluster-Containing Metallothionein

• Project/Area Number: 22655017
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Inorganic chemistry
• Research Institution: Osaka University
• Principal Investigator: HAYASHI Takashi 大阪大学, 大学院・工学研究科, 教授 (20222226)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,520,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: タンパク質 / 酵素 / 鉄硫黄クラスター / 触媒

Research Abstract

The reduction of hydrazine derivatives by a synthetic metallothionein complex with iron ions was monitored. Particularly, the complex is found to bring about the reductive N-N bond cleavage of methyl red smoothly. In addition, the functional model of hydrogenase with iron-sulfur cluster was also prepared by the conjugation between apocytochrome c matrix and iron-carbonyl cluster. The obtained complex exhibits the hydrogenase activity toward the H2 generation in aqueous media.

Research Products

• [Journal Article] Photocatalytic Hydrogen Evolution by a Diiron Hydrogenase Model Based on a Peptide Fragment of Cytochrome c_<556> with an Attached Diiron Carbonyl Cluster and an Attached Ruthenium Photosensitizer (2012)
  • Author(s): Yohei Sano, Akira Onoda, and Takashi Hayashi
  • Journal Title: J. Inorg. Biochem Volume: 108 Pages: 159-162
  • Peer Reviewed
• [Journal Article] Photocatalytic Hydrogen Evolution by a Diiron Hydrogenase Model Based on a Peptide Fragment of Cytochrome c_<556> with an Attached Diiron Carbonyl Cluster and an Attached Ruthenium Photosensitizer (2012)
  • Author(s): Y.Sano, A.Onoda, T.Hayashi
  • Journal Title: JOURNAL OF INORGANIC BIOCHEMISTRY Volume: VOL.108 Pages: 159-162
  • DOI: 10.1016/j.jinorgbio.2011.07.010
  • Peer Reviewed
• [Journal Article] A Hydrogenase Model Ssystem Based on the Sequence of Cytochrome c : Photochemical Hydrogen Evolution in Aqueous Media Chem (2011)
  • Author(s): Yohei Sano, Akira Onoda, and Takashi Hayashi
  • Journal Title: Commun Volume: 47 Pages: 8229-8231
  • Peer Reviewed
• [Journal Article] Preparation and Reactivity of a Tetranuclear Fe(II) Core in the Metallothioneinα-Domain J (2011)
  • Author(s): Yohei Sano, Akira Onoda, Rie Sakurai, Hiroaki Kitagishi, and Takashi Hayashi
  • Journal Title: Inorg. Biochem Volume: 105 Pages: 702-708
  • Peer Reviewed
• [Journal Article] Preparation and Reactivity of a Tetranuclear Fe(II) Core in the Metallothionein α-Domain (2011)
  • Author(s): Yohei Sano
  • Journal Title: J. Inorg. Biochem. Volume: 105 Issue: 5 Pages: 702-708
  • DOI: 10.1016/j.jinorgbio.2011.01.011
  • Peer Reviewed
• [Journal Article] A Hydrogenase Model System Based on the Sequence of Cytochrome c.Photochemical Hydrogen Evolution in Aqueous Media (2011)
  • Author(s): Y.Sano, A.Onoda, T.Hayashi
  • Journal Title: CHEMICAL COMMUNICATIONS Volume: VOL.47 Issue: 29 Pages: 8229-8231
  • DOI: 10.1039/c1cc11157d
  • Peer Reviewed
• [Journal Article] Preparation and Reactivity of a Tetranuclear Fe (II) Core in the Metallothionein α-Domain (2011)
  • Author(s): Yohei Sano, et al
  • Journal Title: J.Inorg.Biochem. Volume: 105 Pages: 702-708
  • Peer Reviewed
• [Journal Article] A Hydrogenase Model Ssystem Based on the Sequence of Cytochrome c : Photochemical Hydrogen Evolution in Aqueous Media (2011)
  • Author(s): Yohei Sano, et al
  • Journal Title: Chem.Commun. Volume: 47 Pages: 8229-8231
  • Peer Reviewed
• [Presentation] Modification and Functionalization of Hemoproteins (2011)
  • Author(s): Takashi Hayashi
  • Organizer: The 11th Global COE International Symposium : Bio-Environmental Chemistry
  • Place of Presentation: Osaka University
  • Year and Date: 2011-12-20
• [Presentation] Modification and Functionalization of Hemoproteins (2011)
  • Author(s): T.Hayashi
  • Organizer: The 11th Global COE International Symposium : Bio-Environmental Chemistry
  • Place of Presentation: 大阪大学銀杏会館(招待講演)
  • Year and Date: 2011-12-20
• [Presentation] シトクロムcを用いた鉄ヒドロゲナーゼ活性中心モデルの構築 (2011)
  • Author(s): 佐野洋平・小野田晃・林高史
  • Organizer: 第61回錯体化学討論会
  • Place of Presentation: 岡山理科大学
  • Year and Date: 2011-09-18
• [Presentation] New Synthetic Model for[ FeFe]-Hydrogenase using Cytochrome c as a Protein Matrix (2011)
  • Author(s): Takashi Hayashi
  • Organizer: 4th Aachen-Osaka Joint Symposium" Direct Catalytic Activation of Inert Substrates"
  • Place of Presentation: Aachen Institute Technology, Germany
  • Year and Date: 2011-09-02
• [Presentation] New Synthetic Model for [FeFe]-Hydrogenase using Cytochrome c as a Protein Matrix (2011)
  • Author(s): T.Hayashi
  • Organizer: 4th Aachen-Osaka Joint Symposium "Direct Catalytic Activation of Inert Substrates"
  • Place of Presentation: Aachen, Germany(招待講演)
  • Year and Date: 2011-09-02
• [Presentation] 鉄ヒドロゲナーゼシステムを指向した人工金属酵素の調製および分子間・分子内電子移動を利用した可視光駆動型水素発生反応 (2011)
  • Author(s): 佐野洋平、小野田晃、林高史
  • Organizer: 第38回生体分子科学討論会
  • Place of Presentation: 筑波大学
  • Year and Date: 2011-06-23
• [Presentation] ヒドロゲナーゼモデルシステムをめざした鉄カルボニルクラスター含有タンパク質の調製および光化学水素発生反応の検討 (2011)
  • Author(s): 佐野洋平・小野田晃・林高史
  • Organizer: 第21回金属の関与する生体関連反応シンポジウム
  • Place of Presentation: 葉大学西千葉キャンパス
  • Year and Date: 2011-05-31
• [Presentation] ヒドロゲナーゼモデルを指向した鉄カルボニルクラスター含有タンパク質の調製と水中における水素発生反応の触媒機能 (2011)
  • Author(s): 佐野洋平・小野田晃・林高史
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学
  • Year and Date: 2011-03-28
• [Remarks]
  • URL: http://www.chem.eng.osaka-u.ac.jp/~hayashiken/index.html