Fabrication of cancer cell-specific nanoparticles utilizing the fluidity of cell membranes

• Project/Area Number: 22655050
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Hokkaido University
• Principal Investigator: NIIKURA Kenichi 北海道大学, 電子科学研究所, 准教授 (40360896)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,420,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: 癌細胞 / 細胞膜 / ナノ粒子合成 / 流動性 / 量子ドット

Research Abstract

For the purpose of the diagnosis of cancer cells, we synthesized nanoparticles that can be selectively uptaken into cell membrane with high fluidity. We can demonstrate that the conformational change of surface ligands on gold nanoparticles can promote the aqueous/organic phase transfer of nanoparticles. The nanoparticles were internalized into cells by penetrating a cell membrane directly. This design of surface ligands based on hydrophilic-hydrophobic block type molecules will lead to the creation of a new type of nanoparticle enabling the detection of cancer cells.

Research Products

• [Journal Article] Gold Nanoparticles Coated with Semi-Fluorinated Oligoethyleneglycol Produce sub-100 nm Nanoparticle Vesicles without Templates (2012)
  • Author(s): K. Niikura, N. Iyo, T. Higuchi, T. Nishio, H. Jinnai, N. Fujitani, K. Ijiro
  • Journal Title: J. Am. Chem. Soc. Volume: 134 Pages: 7632-7632
  • Peer Reviewed
• [Journal Article] Virus Capsid Coating of Gold Nanoparticles via Cysteine-Au Interactions and their Effective Cellular Uptakes (2012)
  • Author(s): K. Nagakawa, K. Niikura, T. Suzuki, Y. Matsuo, M. Igarashi, H. Sawa, K. Ijiro
  • Journal Title: Chem. Lett. Volume: 41(1) Pages: 113-115
  • NAID: 10030222850
  • Peer Reviewed
• [Journal Article] Nuclear Transport facilitated by the Interaction Between Nuclear Pores and Carbohydrates (2012)
  • Author(s): S. Sekiguchi, K. Niikura, Y. Matsuo, Shige H. Yoshimura, K. Ijiro
  • Journal Title: RSC Advances Volume: 2 Pages: 1656-1662
  • Peer Reviewed
• [Journal Article] Hydrophilic Gold Nanoparticles Adaptable for Hydrophobic Solvents (2012)
  • Author(s): S. Sekiguchi, K. Niikura, Y. Matsuo, and K. Ijiro
  • Journal Title: Langmuir Volume: 28(13) Pages: 5503-5507
  • Peer Reviewed
• [Journal Article] Virus capsid coating of gold nanoparticles via cysteine-Au interactions and their effective cellular uptakes (2012)
  • Author(s): Keita Nagakawa, Keinchi Niikura, Tadaki Suzuki, Yasutaka Matsuo, Manabu Igarashi, Hirofumi Sawa, Kuniharu Ijiro
  • Journal Title: Chemistry Letters Volume: 41 Issue: 1 Pages: 113-115
  • DOI: 10.1246/cl.2012.113
  • NAID: 10030222850
  • Peer Reviewed
• [Journal Article] Hydrophilic Gold Nanoparticles Adaptable for Hydrophobic Solvents (2012)
  • Author(s): S.Sekiguchi, K.Niikura, Y.Matsuo, K.Ijiro
  • Journal Title: Langmuir Volume: 28 Issue: 13 Pages: 5503-5507
  • DOI: 10.1021/la300299x
  • Peer Reviewed
• [Journal Article] Transfection Activity by Controlling Intracellular Trafficking : The Function of GALA and Maltotriose (2011)
  • Author(s): H. Akita, T. Masuda, T. Nishio, K. Niikura, K. Ijiro, and H. Harashima
  • Journal Title: Mol. Pharmaceutics Volume: 8(4) Pages: 1436-1442
  • Peer Reviewed
• [Journal Article] Artificial Polymeric Receptors on the Cell Surface Promote the Efficient Cellular Uptake of Quantum Dots (2011)
  • Author(s): K. Niikura, K. Nambara, T. Okajima, R. Kamitani, S. Aoki, Y. Matsuo, and K. Ijiro
  • Journal Title: Organic and Biomolecular Chemistry Volume: 9 Pages: 5787-5792
  • Peer Reviewed
• [Journal Article] pH-Dependent Network Formation of Quantum Dots and Fluorescent Quenching by Au Nanoparticle Embedding (2011)
  • Author(s): S. Sekiguchi, K. Niikura, N. Iyo, Y. Matsuo, A. Eguchi, T. Nakabayashi, N. Ohta and K. Ijiro
  • Journal Title: ACS Applied Materials & Interfaces Volume: 3(11) Pages: 4169-4173
  • Peer Reviewed
• [Journal Article] Improving in vivo hepatic transfection activity by controlling intracellular trafficking : the function of GALA and maltotriose (2011)
  • Author(s): Akita H, Masuda T, Nishio T, Niikura K, Ijiro K, Harashima H
  • Journal Title: Mol Pharm Volume: 8(4) Issue: 4 Pages: 1436-1442
  • DOI: 10.1021/mp200189s
  • Peer Reviewed
• [Journal Article] Influence of Hydrophobic Structures on the Plasma Membrane Permeability of Lipid-like Molecules (2010)
  • Author(s): K. Niikura, K. Nambara, T. Okajima, Y. Matsuo, K. Ijiro
  • Journal Title: Langmuir Volume: 26(12) Pages: 9170-9175
  • Peer Reviewed
• [Journal Article] Self-lubricating Nanoparticles : Self-Organization into 3D-Superlattices during a Fast Drying Process (2010)
  • Author(s): T. Nishio, K. Niikura, Y. Matsuo and K. Ijiro
  • Journal Title: Chem. Commun. Volume: 46 Pages: 8977-8979
  • Peer Reviewed
• [Journal Article] Self-assembled Hierarchic Structures of Metal-Molecule Hybrids for Sensing and Electronic Devices (2010)
  • Author(s): K.Ijiro
  • Journal Title: ICEP 2010 Proceedings Pages: 179-184
  • Peer Reviewed
• [Journal Article] Self-lubricating Nanoparticles : Self-Organization into 3D-Superlattices during a Fast Drying Process (2010)
  • Author(s): T.Nishio
  • Journal Title: Chem.Commun. Volume: 46 Pages: 8977-8979
  • Peer Reviewed
• [Presentation] SERS Active Hollow Assembly of Gold Nanoparticles in Solutions (2011)
  • Author(s): N. Iyo
  • Organizer: 12th RIES-Hokudai International Symposium"観"
  • Place of Presentation: Chateraise Gateaux Kingdom Sapporo(北海道札幌市)
  • Year and Date: 2011-11-21
• [Presentation] SERS Active Hollow Assembly of Gold Nanoparticles in Solutions (2011)
  • Author(s): N.Iyo
  • Organizer: 12th RIES-Hokudai International Symposium"観"[KAN]
  • Place of Presentation: Chateraise Gateaux Kingdom Sapporo(北海道札幌市)
  • Year and Date: 2011-11-21
• [Presentation] フッ素系チオールで保護されたナノ粒子の自己組織化 (2011)
  • Author(s): 新倉謙一
  • Organizer: 第60回高分子討論会
  • Place of Presentation: 岡山大学
  • Year and Date: 2011-09-28
• [Presentation] フッ素系チオールで保護されたナノ粒子の自己組織化 (2011)
  • Author(s): 新倉謙一
  • Organizer: 第60回高分子討論会
  • Place of Presentation: 岡山大学津島キャンパス(岡山県岡山市)
  • Year and Date: 2011-09-28
• [Presentation] フッ素界面の導入による金ナノ粒子配列構造の高速製作 (2011)
  • Author(s): 新倉謙一
  • Organizer: 第63回コロイドおよび界面化学討論会
  • Place of Presentation: 京都大学
  • Year and Date: 2011-09-09
• [Presentation] フッ素界面の導入による金ナノ粒子配列構造の高速製作 (2011)
  • Author(s): 新倉謙一
  • Organizer: 第63回コロイドおよび界面化学討論会
  • Place of Presentation: 京都大学吉田キャンパス(京都府京都市)
  • Year and Date: 2011-09-09
• [Presentation] The Nuclear Import of Nanoparticles by Displaying Glycoside Cluster on the Surface (2011)
  • Author(s): S. Sekiguchi
  • Organizer: Glyco 21
  • Place of Presentation: University of Vienna(Australia)
  • Year and Date: 2011-08-26
• [Presentation] 有機フッ素化合物提示金ナノ粒子の自己組織化による三次元球形構造体の作製 (2011)
  • Author(s): 伊與直希
  • Organizer: 2011年度北海道高分子若手研究会
  • Place of Presentation: 洞爺サンパレス(北海道)
  • Year and Date: 2011-08-26
• [Presentation] 様々な形状の金ナノ粒子ワクチンの作製とワクチン活性の形状依存性 (2011)
  • Author(s): 松永達也
  • Organizer: 2011年度北海道高分子若手研究会
  • Place of Presentation: 洞爺サンパレス(北海道)
  • Year and Date: 2011-08-26
• [Presentation] The Nuclear Import of Nanoparticles by Displaying Glycoside Cluster on the Surface (2011)
  • Author(s): S.Sekiguchi
  • Organizer: 21st international symposium on glycoconjugates (Glyco 21)
  • Place of Presentation: University of Vienna(オーストリア)
  • Year and Date: 2011-08-26
• [Presentation] 有機フッ素化合物提示金ナノ粒子の自己組織化による三次元球形構造体の作製 (2011)
  • Author(s): 伊與直希
  • Organizer: 2011年度北海道高分子若手研究会
  • Place of Presentation: 洞爺サンパレス(北海道有珠郡壮瞥町)
  • Year and Date: 2011-08-26
• [Presentation] 様々な形状の金ナノ粒子ワクチンの作製とワクチン活性の形状依存性 (2011)
  • Author(s): 松永達也
  • Organizer: 2011年度北海道高分子若手研究会
  • Place of Presentation: 洞爺サンパレス(北海道有珠郡壮瞥町)
  • Year and Date: 2011-08-26
• [Presentation] 細胞膜修飾高分子を用いたガン細胞特異的な微粒子導入 (2011)
  • Author(s): 新倉謙一
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学
  • Year and Date: 2011-03-26
• [Presentation] 細胞膜修飾高分子を用いたガン細胞特異的な微粒子導入 (2011)
  • Author(s): 新倉謙一
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(横浜市)
  • Year and Date: 2011-03-26
• [Presentation] Effect of hydrophobic structures of prodrugs on cellular membrane permeability (2010)
  • Author(s): K. Nambara
  • Organizer: Pacifichem 2010
  • Place of Presentation: Hawaii
  • Year and Date: 2010-12-17
• [Presentation] Effect of hydrophobic structures of prodrugs on cellular membrane permeability (2010)
  • Author(s): K.Nambara
  • Organizer: 環太平洋国際化学会議(Pacifichem2010)
  • Place of Presentation: ハワイ(米国)
  • Year and Date: 2010-12-17
• [Presentation] 細胞内刺激に応答して分子放出可能なウイルスカプセルの作製 (2010)
  • Author(s): 新倉謙一
  • Organizer: 第7回バイオオプティクス研究会・理研シンポジウム「蛍光相関分光と情報伝達(7)」合同シンポジウム
  • Place of Presentation: 東京農工大学
  • Year and Date: 2010-12-03
• [Presentation] 細胞内刺激に応答して分子放出可能なウイルスカプセルの作製 (2010)
  • Author(s): 新倉謙一
  • Organizer: 第7回バイオオプティクス研究会・理研シンポジウム「蛍光相関分光と情報伝達』(7)」合同シンポジウム
  • Place of Presentation: 東京農工大学(東京都府中市) 招待講演
  • Year and Date: 2010-12-03
• [Presentation] Carbohydrate Modification of Nanoparticles Allows the Rapid Passage through the Nuclear Pore Barrier (2010)
  • Author(s): K. Niikura
  • Organizer: AsiaNANO2010
  • Place of Presentation: National Museum of Emerging Science and Innovation
  • Year and Date: 2010-11-02
• [Presentation] Efficient Delivery of Nanoparticles into Cancer Cells Facilitated by Cellular Membrane-Retaining Cationic Polymers with Artificial Ligands (2010)
  • Author(s): K. Nambara
  • Organizer: AsiaNANO2010
  • Place of Presentation: National Museum of Emerging Science and Innovation
  • Year and Date: 2010-11-02
• [Presentation] Efficient Delivery of Nanoparticles into Cancer Cells Facilitated by Cellular Membrane-Retaining Cationic Polymers with Artificial Ligands (2010)
  • Author(s): K.Nambara
  • Organizer: Asia NANO 2010 (Asian Conference on Nanoscience and Nanotechnology)
  • Place of Presentation: Miraikan (National Museum of Emerging Science and Innovation) (東京都)
  • Year and Date: 2010-11-02
• [Presentation] Carbohydrate Modification of Nanoparticles Allows the Rapid Passage through the Nuclear Pore Barrier (2010)
  • Author(s): K.Niikura
  • Organizer: Asia NANO 2010 (Asian Conference on Nanoscience and Nanotechnology)
  • Place of Presentation: Miraikan (National Museum of Emerging Science and Innovation) (東京都)
  • Year and Date: 2010-11-02
• [Presentation] 物質の細胞膜透過における疎水部構造の影響 (2010)
  • Author(s): 南原克行
  • Organizer: 第59回高分子討論会
  • Place of Presentation: 北海道大学
  • Year and Date: 2010-09-16
• [Presentation] 細胞表層修飾可能な高分子を用いたガン細胞への高効率な物質導入 (2010)
  • Author(s): 南原克行
  • Organizer: 第59回高分子討論会
  • Place of Presentation: 北海道大学
  • Year and Date: 2010-09-16
• [Presentation] 細胞表層修飾可能な高分子を用いたガン細胞への高効率な物質導入 (2010)
  • Author(s): 南原克行
  • Organizer: 第59回高分子討論会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2010-09-16
• [Presentation] 物質の細胞膜透過における疎水部構造の影響 (2010)
  • Author(s): 南原克行
  • Organizer: 第59回高分子討論会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2010-09-16
• [Presentation] Mechanism for nuclear import of maltooligosaccharide-coated nanoparticles (2010)
  • Author(s): S. Sekiguchi
  • Organizer: ICS2010
  • Place of Presentation: Makuhari Messe International Convention Comple
  • Year and Date: 2010-08-02
• [Presentation] Mechanism for nuclear import of maltooligosaccharide-coated nanoparticles (2010)
  • Author(s): S.Sekiguchi
  • Organizer: The 25th International Carbohydrate Symposium (ICS2010)
  • Place of Presentation: Makuhari Messe International Convention Comple(千葉県)
  • Year and Date: 2010-08-02
• [Presentation] Design of Hydrophobic Structures of Prodrugs to Enhance Cellular Membrane Permeability (2010)
  • Author(s): K. Nambara
  • Organizer: LB13
  • Place of Presentation: Quebec City(Canada)
  • Year and Date: 2010-07-20
• [Presentation] Design of Hydrophobic Structures of Prodrugs to Enhance Cellular Membrane Permeability (2010)
  • Author(s): K.Nambara
  • Organizer: 13th International Conference on Organized Molecular Films (LB13)
  • Place of Presentation: Quebec City (カナダ)
  • Year and Date: 2010-07-20
• [Presentation] 糖鎖修飾により促進されるナノ粒子の核移行とメカニズム解明 (2010)
  • Author(s): 新倉謙一
  • Organizer: 第59回高分子学会年次大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2010-05-27
• [Presentation] 糖鎖修飾により促進されるナノ粒子の核移行とメカニズム解明 (2010)
  • Author(s): 新倉謙一
  • Organizer: 第59回高分子学会年次大会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2010-05-27
• [Presentation] ウイルスの金属被覆とウイルスセンシングへの応用 (2010)
  • Author(s): 新倉謙一
  • Organizer: TKGグループ研究助成金受賞記念講演会
  • Place of Presentation: 田中貴金属工業(株)本社(東京)
  • Year and Date: 2010-04-27
• [Presentation] ウイルスの金属被覆とウイルスセンシングへの応用 (2010)
  • Author(s): 新倉謙一
  • Organizer: TKGグループ研究助成金 受賞記念講演会
  • Place of Presentation: 田中貴金属工業(株)本社(東京都) 招待講演
  • Year and Date: 2010-04-27
• [Book] 高分子 (2011)
  • Author(s): 新倉謙一
  • Publisher: 社団法人高分子学会
• [Book] 生体の科学 (2011)
  • Author(s): 新倉謙一
  • Publisher: 財団法人金原一郎記念医学医療振興財団
• [Book] 高分子2011年6月号(Vol.60 No.8) (2011)
  • Author(s): 新倉謙一
  • Publisher: 社団法人高分子学会
• [Book] 生体の科学2011年10月号(増大号)(Vol.62 No.5) (2011)
  • Author(s): 新倉謙一
  • Publisher: 財団法人金原一郎記念医学医療振興財団
• [Patent(Industrial Property Rights)] 自己組織化可能な粒子、金属コアの表面修飾剤、粒子集積体、粒子集積体の製造方法、光増強素子及び光化学反応を利用する装置 (2011)
  • Inventor(s): 新倉謙一
  • Industrial Property Rights Holder: 北海道大学
  • Industrial Property Number: 2011-036468
  • Filing Date: 2011-02-22