Development about photo-triggered autonomous DNA computing

• Project/Area Number: 22655051
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Japan Advanced Institute of Science and Technology
• Principal Investigator: FUJIMOTO Kenzo 北陸先端科学技術大学院大学, マテリアルサイエンス研究科, 教授 (90293894)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,480,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: DNA / 光ライゲーション / 光クロスリンク / クロスリンク / 分子計算

Research Abstract

We demonstrate that normal K-ras gene suppressed selectively by photo-triggered autonomous DNA computing and calculated survival rate by real time clamp PCR. BxPC-3 and Capan-1 were suppressed by photo-triggered autonomous DNA Ccamp respectively 86%, 47%.

Research Products

• [Journal Article] Stabilization of DNA nanostructures by photo-cross-linking (2011)
  • Author(s): Miho Tagawa, Koh-ichiroh Shohda, Kenzo Fujimoto and Akira Suyama
  • Journal Title: Soft Matter Volume: 7 Pages: 10931-10934
  • Peer Reviewed
• [Journal Article] Construction of branched DNA for SNP determination on glass-chip using photochemical ligation (2011)
  • Author(s): Minhaz Uddin Ahmed, Yoshinaga Yoshimura, M. Mosharraf Hossain, Eiichi Tamiya and Kenzo Fujimoto
  • Journal Title: Bio Chip J. Volume: 5 Pages: 206-221
  • Peer Reviewed
• [Journal Article] Signal turn-on probe for nucleic acid detection based on 19F nuclear magnetic resonance (2011)
  • Author(s): Takashi Sakamoto, Yu-ki Shimizu, Jun Sasaki, Hikaru Hayakawa and Kenzo Fujimoto
  • Journal Title: Bioorg Med. Chem. Lett Volume: 21 Pages: 303-206
  • Peer Reviewed
• [Journal Article] Signal turn-on probe for nucleic acid detection based on 19F nuclear magnetic resonance (2011)
  • Author(s): Takashi Sakamoto, Yu-ki Shimizu, Jun Sasaki, Hikaru Hayakawa, Kenzo Fujimoto
  • Journal Title: Bioorg.Med.Chem.Lett Volume: 21 Issue: 1 Pages: 303-206
  • DOI: 10.1016/j.bmcl.2010.11.013
  • Peer Reviewed
• [Journal Article] Construction of branched DNA for SNP determination on glass-chip using photochemical ligation (2011)
  • Author(s): Minhaz Uddin Ahmed, Yoshinaga Yoshimura, M.Mosharraf Hossain, Eiichi Tamiya, Kenzo Fujimoto
  • Journal Title: BioChip J. Volume: 5 Issue: 3 Pages: 206-221
  • DOI: 10.1007/s13206-011-5303-0
  • Peer Reviewed
• [Journal Article] Possibility of genetic restoration for a disease treatment (2011)
  • Author(s): Toshifumi Tsukahara, Yu Ooka, Shafiul Alam, Hitoshi Suzuki, Kenzo Fujimoto
  • Journal Title: IEEE ICMSAO Volume: 4 Pages: 1-4
  • DOI: 10.1109/icmsao.2011.5775517
  • Peer Reviewed
• [Journal Article] Site-Specific Photochemical RNA Editing (2010)
  • Author(s): Kenzo Fujimoto, Kaoru Konishi-Hiratsuka, Takashi Sakamoto and Yoshinaga Yoshimura
  • Journal Title: Chemical Communications Volume: 46 Pages: 7545-7548
  • Peer Reviewed
• [Journal Article] The Site-Specific Cytosineto the Uracil Transition Using Reversible DNA Photocrosslinking (2010)
  • Author(s): Kenzo Fujimoto, Takashi Sakamoto, Kaoru Konishi-Hiratsuka and Yoshinaga Yoshimura
  • Journal Title: Chem Bio Chem Volume: 11 Pages: 1661-1664
  • Peer Reviewed
• [Journal Article] The Site-Specific Cytosine to the Uracil Transition Using Reversible DNA Photocrosslinking (2010)
  • Author(s): Kenzo Fujimoto, Takashi Sakamoto, Kaoru Konishi-Hiratsuka, Yoshinaga Yoshimura
  • Journal Title: ChemBioChem Volume: 11 Pages: 1661-1664
  • Peer Reviewed
• [Journal Article] Photoreversible DNA end capping for the formation of hairpin structures (2010)
  • Author(s): Yoshinaga Yoshimura, Hajime Okada , Kenzo Fujimoto
  • Journal Title: Organic & Biomolecular Chemistry Volume: 8 Pages: 1523-1526
  • Peer Reviewed
• [Journal Article] Surface-enhanced Raman spectroscopy for facile DNA detection using gold nanoparticle aggregates formed via photo ligation (2010)
  • Author(s): Nguyen T.B.Thuy, Ryoko Yokogawa, Yoshinaga Yoshimura, Kenzo Fujimoto, Mikio Koyano, Shinya Maenosono
  • Journal Title: Analyst Volume: 135 Pages: 85-89
  • Peer Reviewed
• [Presentation] D Development of Photochemical DNA and RNA Manipulation (2011)
  • Author(s): Fujimoto Kenzo
  • Organizer: 34th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Pacifico YOKOHAMA(神奈川県)
  • Year and Date: 2011-12-13
• [Presentation] Development of Photochemical DNA and RNA Manipulation (2011)
  • Author(s): Kenzo Fujimoto
  • Organizer: 34th Annual Meeting of the Molecular Biology Society of Japan (Invited)
  • Place of Presentation: Pacifico YOKOHAMA (神奈川県)
  • Year and Date: 2011-12-13
• [Presentation] Regulation of gene expression by photocrosslinking antisense oligonucleotide containing of carbazole derivative (2011)
  • Author(s): Atsuo Shigeno, Takashi Sakamoto, Kenzo Fujimoto
  • Organizer: 34th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Pacifico YOKOHAMA, (神奈川県)
  • Year and Date: 2011-12-13
• [Presentation] 酵素を使わずに化学の力でDNAを操る (2011)
  • Author(s): 藤本健造
  • Organizer: 日本化学会東北支部主催会員増強のための講演会
  • Place of Presentation: 東北大学(宮城県)
  • Year and Date: 2011-11-25
• [Presentation] 酵素を使わずに化学の力でDNAを操る (2011)
  • Author(s): 藤本健造
  • Organizer: 日本化学会東北支部主催会員増強のための講演会
  • Place of Presentation: 東北大学(宮城県)(招待)
  • Year and Date: 2011-11-25
• [Presentation] Rice genome screening by photo-reactive DNA probes (2011)
  • Author(s): Takematsu Yuuki, Yoshinaga Yoshimura, Sakamoto Takashi, Fujimoto Kenzo
  • Organizer: The 38th International Symposium on Nucleic Acid Chemistry
  • Place of Presentation: Hokkaido Univ.(北海道)
  • Year and Date: 2011-11-09
• [Presentation] Site-specific transition of cytosine to uracil by reversible DNA and RNA photo-crosslinking (2011)
  • Author(s): Futamura Daiki Yoshinaga Yoshimura, Sakamoto Takashi, Fujimoto Kenzo
  • Organizer: The 38th International Symposium on Nucleic Acid Chemistry
  • Place of Presentation: Hokkaido Univ.(北海道)
  • Year and Date: 2011-11-09
• [Presentation] Development of Photo triggered DNA and RNA Manipulation (2011)
  • Author(s): Fujimoto Kenzo
  • Organizer: 5th Pacific Symposium on Radical Chemistry
  • Place of Presentation: Shirahama,(和歌山県)
  • Year and Date: 2011-09-27
• [Presentation] Photochemical DNA manipulation toward for DNA computing (2011)
  • Author(s): Nakamura Shigetaka, Ogasakawa Shinzi and Fujimoto Kenzo
  • Organizer: 17th International Conference on DNA Computing and Molecular Programming
  • Place of Presentation: LA, USA
  • Year and Date: 2011-09-22
• [Presentation] Photochemical DNA manipulation toward for DNA computing (2011)
  • Author(s): Nakamura Shigetaka, Ogasa kawa Shinzi, Fujimoto Kenzo
  • Organizer: 17th International Conference on.DNA Computing and Molecular Programming
  • Place of Presentation: LA, USA
  • Year and Date: 2011-09-22
• [Presentation] 光化学的な新規遺伝子操作法の開発とその応用 (2011)
  • Author(s): 藤本健造
  • Organizer: 日本化学会中国四国支部愛媛地区化学講演会
  • Place of Presentation: 愛媛大学(愛媛県)
  • Year and Date: 2011-09-14
• [Presentation] 光化学的な新規遺伝子操作法の開発とその応用 (2011)
  • Author(s): 藤本健造
  • Organizer: 日本化学会中国四国支部愛媛地区化学講演会
  • Place of Presentation: 愛媛大学(愛媛県)(招待)
  • Year and Date: 2011-09-14
• [Presentation] 光応答性人工核酸分子の合成と応用 (2011)
  • Author(s): 藤本健造
  • Organizer: 第28回有機合成化学セミナー
  • Place of Presentation: 天童ホテル(山形県)(招待)
  • Year and Date: 2011-08-31
• [Presentation] Catalytic Repair of a Thymine Dimer in DNA via Carbazole Nucleoside : Its Application for DNA nanotechnology (2011)
  • Author(s): Nakamura Shigetaka, Ogasakawa Shinzi, Fujimoto Kenzo
  • Organizer: International Conference of Photochemistry 2011
  • Place of Presentation: Beijing, China
  • Year and Date: 2011-08-06
• [Presentation] Photochemical site specific DNA and RNA transition (2011)
  • Author(s): Fujimoto Kezno, Hiratsuka Kaoru, Shigeno Atsuo
  • Organizer: 12th Tetrahedron Symposium
  • Place of Presentation: Seiches, Spain
  • Year and Date: 2011-06-23
• [Presentation] Development of Phototriggered DNA and RNA Manipulation (2011)
  • Author(s): Kenzo Fujimoto
  • Organizer: 5th Pacific Symposium on Radical Chemistry (Invited)
  • Place of Presentation: Shirahama, WAKAYAMA
• [Presentation] DNA computing based on photochemical DNA manipulation (2010)
  • Author(s): Shigetaka Nakamura, Shinji Ogasawara , Kenzo Fujimoto
  • Organizer: Pacifichem 2010
  • Place of Presentation: USA(Hawaii)
  • Year and Date: 2010-12-17
• [Presentation] Photoreactive Oligonucleotide Containing Cyanovinylcarbazole for the Regulation of Point Mutated K-rasgene expression (2010)
  • Author(s): Atsuo Shigeno, Takashi Sakamoto, Yoshinaga Yoshimura, Kenzo Fujimoto
  • Organizer: Pacifichem 2010
  • Place of Presentation: USA(Hawaii)
  • Year and Date: 2010-12-17
• [Presentation] Site-Specific Photochemical DNA and RNA editing (2010)
  • Author(s): Kenzo Fujimoto
  • Organizer: The 37th International Symposium on Nucleic Acids Chemistry 2010
  • Place of Presentation: Yokohama
  • Year and Date: 2010-11-12
• [Presentation] Development of 19F-MR Signal Turn-on Probe for Gene Expression in vivo (2010)
  • Author(s): Takashi Sakamoto, Yuuki Shimizu, Kenzo Fujimoto
  • Organizer: The 37th International Symposium on Nucleic Acids Chemistry 2010
  • Place of Presentation: Yokohama(Invited)
  • Year and Date: 2010-11-12
• [Presentation] Photo-reactive Oligonucleotide Containing Cyanovinylcarbazole for the Regulation of Point Mutated K-ras gene (2010)
  • Author(s): Atsuo Shigeno, Takashi Sakamoto, Yoshinaga Yoshimura , Kenzo Fujimoto
  • Organizer: The 37th International Symposium on Nucleic Acids Chemistry 2010
  • Place of Presentation: Yokohama
  • Year and Date: 2010-11-12
• [Presentation] シアノビニルカルバゾール修飾オリゴDNAを用いた光化学的DNA編集 (2010)
  • Author(s): 坂本隆・平塚薫・吉村嘉永・藤本健造
  • Organizer: 第4回バイオ関連化学シンポジウム
  • Place of Presentation: 大阪大学
  • Year and Date: 2010-09-24
• [Presentation] 遺伝子発現のin vivoイメージングを目指したフッ素核磁気共鳴プローブの開発 (2010)
  • Author(s): 坂本隆・清水勇喜・佐々木淳・早川輝・藤本健造
  • Organizer: 第4回バイオ関連化学シンポジウム
  • Place of Presentation: 木阪大学
  • Year and Date: 2010-09-24
• [Presentation] 光化学的DNA操作によるDNAコンピューティング (2010)
  • Author(s): 小笠原慎治・坂本隆・中村重孝・藤本健造
  • Organizer: 第48回生物物理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 2010-09-20
• [Presentation] 光反応による機能性RNAの検出・同定技術の開発 (2010)
  • Author(s): 藤本健造
  • Organizer: 機能性RNAプロジェクト講演会
  • Place of Presentation: USA(Hawau)
  • Year and Date: 2010-07-17
• [Presentation] 光化学的な遺伝子操作法の開発とDNA分子プログラミングへの応用 (2010)
  • Author(s): 藤本健造
  • Organizer: 第4回生物物質科学フォーラム
  • Place of Presentation: 東京キャンパスイノベーションセンター(招待講演)
  • Year and Date: 2010-06-15