Exploring the possibilities of genome recombination
Project/Area Number |
22655053
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Chemistry related to living body
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Research Institution | Kyoto University |
Principal Investigator |
ATOMI Haruyuki 京都大学, 大学院・工学研究科, 教授 (90243047)
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Project Period (FY) |
2010 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥3,420,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,600,000 (Direct Cost: ¥1,600,000)
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Keywords | 遺伝子組換え / 超好熱菌 / メタゲノム / ゲノム組換え / 合成生物学 / 始原菌 / Thermococcus kodakaraensis / ゲノム |
Research Abstract |
Focusing on the high transformation efficiency of the hyperthermophilic archaeon Thermococcus kodakarensis, we explored the possibilities of performing wide-scale DNA recombination. Mutant strains displaying uracil or tryptophan auxotrophy were used as host cells, and selection was based on their prototrophy. We were able to obtain prototrophs using the genomic DNA of wild-type T. kodakarensis. By inserting a small DNA fragment from the DNA donor beforehand, we were able to obtain transformants using genomic DNA from other hyperthermophiles.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Insights into the evolution of Archaea and eukaryotic protein modifier systems revealed by the genome of a novel archaeal group2011
Author(s)
Nunoura T, Takaki Y, Kakuta J, Nishi S, Sugahara J, Kazama H, Chee G-J, Hattori M, Kanai A, Atomi H, Takai K, Takami H
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Journal Title
Nucleic Acids Research
Volume: 39
Pages: 3204-3223
Related Report
Peer Reviewed
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[Journal Article] His tone and TK0471/TrmBL2 form a novel heterogeneous genome architecture in the hyperthermophilic archaeon The rmococcus kodakarensis2011
Author(s)
Maruyama, H., Shin, M., Oda, T., Matsumi, R., Ohniwa, R. L., Itoh, T., Shirahige, K., Imanaka, T., Atomi, H., Yoshimura, S. H., Takeyasu, K
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Journal Title
Molecular Biology of the Cell
Volume: 22(3)
Pages: 386-398
Related Report
Peer Reviewed
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[Journal Article] Insights into the evolution of Archaea and eukaryotic protein modifier systems revealed by the genome of a novel archaeal group.2011
Author(s)
Nunoura T, Takaki Y, Kakuta J, Nishi S, Sugahara J, Kazama H, Chee G-J, Hattori M, Kanai A, Atomi H, Takai K, Takami H.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] For mate-driven growth coupled with hydrogen production2010
Author(s)
Kim, Y. J., Lee, H. S., Kim, E. S., Bae, S. S., Lim, J. K., Matsumi, R., Lebedinsky, A. V., Sokolova, T. G., Kozhevnikova, D. A., Cha, S. S., Kim, S. J., Kwon, K. K., Imanaka, T., Atomi, H., Bonch-Osmolovskaya, E. A., Lee, J. H., Kang, S. G
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Journal Title
Nature
Volume: 497(7313)
Pages: 352-355
Related Report
Peer Reviewed
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[Journal Article] Formate-driven growth coupled with H2 production.2010
Author(s)
Kim YJ, Lee HS, Kim ES, Bae SS, Lim JK, Matsumi R., Lebedinsky AV, Sokolova TG, Kozhevnikova DA, Cha SS, Kim SJ, Kwon KK, Imanaka T, Atomi H, Bonch-Osmolovskaya EA, Lee JH, Kang SG.
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Journal Title
Nature
Volume: 467
Pages: 352-355
Related Report
Peer Reviewed
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