Reaction Control of Nanoreactor by Utilizing DNA Hybridization
| Project/Area Number |
22656179
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Properties in chemical engineering process/Transfer operation/Unit operation
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| Research Institution | Kyushu University |
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Principal Investigator |
GOTO Masahiro 九州大学, 工学研究院, 教授教授 (10211921)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,590,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | ベシクル / ナノレアクター / 分子集合体 / ハイブリダイゼーション / DNA / ナノリアクター / 分子認識 / タンパク質連結 / トロンビン / 相補鎖 / 配列認識 / 酵素反応 / リポソーム / バイオセンサー |
|---|
| Research Abstract |
We investigated DNA-directed aggregation of vesicles using DNA-surfactants. Following tethering of single-stranded DNA oligonucleotides to vesicles using DNA-surfactant, the tethered vesicles were assembled with other vesicles bearing complementary strands. The vesicle aggregation was strongly affected by the salt concentration and by temperature according to the characteristics of DNA hybridization. Restriction enzyme, which can hydrolyze the double-stranded DNA used in the present study, dissociated the vesicle aggregates. Exploration using fluorescently labeled vesicles suggested that the DNA-directed vesicle aggregation took place in a sequence-specific manner through DNA-duplex formation. Interestingly, the DNA-directed aggregation using short DNA-surfactant induced the fusion of vesicles to produce giant vesicles, resulting in an enzymatic reaction in the giant vesicle.
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Report
(3 results)
Research Products
(24 results)
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[Journal Article] Short time ionic liquids pretreatment on lignocellulosic biomass to enhance enzymatic saccharification2012
Author(s)
Uju, Y. Shoda, A. Nakamoto, M. Goto, W. Tokuhara, Y. Noritake, S. Katahira, N. Ishida, K. Nakashima, C. Ogino, N. Kamiya
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Journal Title
Bioresour. Technol
Volume: 103
Pages: 446-452
Related Report
Peer Reviewed
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