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On-demand and non-invasive cell detachment technology

Research Project

Project/Area Number 22656187
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Biofunction/Bioprocess
Research InstitutionUniversity of Tsukuba

Principal Investigator

FUKUDA Junji  筑波大学, 数理物質系, 講師 (80431675)

Co-Investigator(Kenkyū-buntansha) SUZUKI Hiroaki  筑波大学, 数理物質系, 教授 (20282337)
Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,440,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywords医用化学工学 / 動物細胞培養 / 自己組織化単分子膜 / 透明電極 / バイオ関連機器 / 細胞チップ / マイクロアレイ
Research Abstract

The aim of this project is to develop a technology for detaching specific cells being observed under a microscopy with single-cell resolution. We first demonstrated that cells on a transparent electrode (ITO electrode) can be rapidly detached by using an electrochemical reaction. We subsequently fabricated a microarray of the electrode so that an electrical potential can be separately applied to the electrodes. This electrode system can be used to detach individual cells one by one.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (50 results)

All 2013 2012 2011 2010 Other

All Journal Article (12 results) (of which Peer Reviewed: 9 results) Presentation (34 results) Remarks (2 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 2 results)

  • [Journal Article] Tissue engineering based on electrochemical desorption of an RGD-containing oligopeptide2013

    • Author(s)
      Naoto Mochizuki, Takahiro Kakegawa, Tatsuya Osaki, Nasser Sadr, Nezamoddin N. Kachouie, Hiroaki Suzuki, Junji Fukuda
    • Journal Title

      Journal of Tissue Engineering and Regenerative Medicine

      Volume: 7 Issue: 3 Pages: 236-243

    • DOI

      10.1002/term.519

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] On-chip diagnosis of subclinical mastitis in cows by electrochemical measurement of neutrophil activity in milk2012

    • Author(s)
      S. Kimura, J. Fukuda, A. Tajima and H. Suzuki
    • Journal Title

      Lab on a Chip (IF=6.26)

      Volume: 12 (7) Issue: 7 Pages: 1309-15

    • DOI

      10.1039/c2lc20952g

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Drug-eluting microarrays for cell-based screening of chemical-induced apoptosis2011

    • Author(s)
      C. H. Kwon, I. Wheeldon, N. N. Kachouie, S. H. Lee, H. Bae, S. Sant, J. Fukuda, J. W. Kang, and A. Khademhosseini
    • Journal Title

      Analytical Chemistry (IF=5.87)

      Volume: 83 (11) Issue: 11 Pages: 4118-25

    • DOI

      10.1021/ac200267t

    • Related Report
      2011 Final Research Report
  • [Journal Article] Spatio-temporal detachment of single cell susing microarrayed transparent electrodes2011

    • Author(s)
      J. Fukuda, Y. Kameoka, H. Suzuki
    • Journal Title

      Biomaterials (IF=7.88)

      Volume: 32(28) Issue: 28 Pages: 6663-9

    • DOI

      10.1016/j.biomaterials.2011.05.068

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Khademhosseini, SAM-based cell transfer to photopatterned hydrogels for microengineering vascular-like structures2011

    • Author(s)
      N. Sadr, M. Zhu, T. Osaki, T. Kakegawa, Y. Yang, M. Moretti, J. Fukuda, A.
    • Journal Title

      Biomaterials (IF=7.88)

      Volume: 32(30) Issue: 30 Pages: 7479-90

    • DOI

      10.1016/j.biomaterials.2011.06.034

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Khademhosseini, Directed assembly of cell-laden microgels for building porous three-dimensional tissue constructs2011

    • Author(s)
      F. Yanagawa, H. Kaji, Y. H. Jang, H. Bae, D. Yanan, J. Fukuda, H. Qi, A.
    • Journal Title

      Khademhosseini, Directed assembly of cell-laden microgels for building porous three-dimensional tissue constructs

      Volume: 97A (1) Issue: 1 Pages: 93-102

    • DOI

      10.1002/jbm.a.33034

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hepatocytespheroid arrays inside microwells connected with microchannels2011

    • Author(s)
      J. Fukuda and K. Nakazawa
    • Journal Title

      Biomicrofluidics(IF=3.90)

      Volume: 5, 022205 Issue: 2

    • DOI

      10.1063/1.3576905

    • Related Report
      2011 Final Research Report
  • [Journal Article] Drug-eluting microarrays for cell-based screening of chemical-induced apoptosis2011

    • Author(s)
      C.H.Kwon, I.Wheeldon, N.N.Kachouie, S.H.Lee, H.Bae, S.Sant, J.Fukuda, J.W.Kang, A.Khademhosseini
    • Journal Title

      Analytical Chemistry

      Volume: in press

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hepatocyte spheroid arrays inside microwells connected with microchanneis2011

    • Author(s)
      J.Fukuda, K.Nakazawa
    • Journal Title

      Biomicrofluidics

      Volume: in press

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Directed assembly of cell-laden microgels for building porous three-dimensional tissue constructs2011

    • Author(s)
      F.Yanagawa, H.Kaji, Y.H.Jang, H.Bae, D.Yanan, J.Fukuda, H.Qi, A.Khademhosseini
    • Journal Title

      Journal of Biomedical Materials Research A

      Volume: 97A(1) Pages: 93-102

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Directed assembly of cell-Laden hydrogels for engineeringfunctional tissues2010

    • Author(s)
      N. Kachouie, Y. Du, H. Bae, M. Khabiry, A. F. Ahari, B. Zamanian, J. Fukuda, and A. Khademhosseini
    • Journal Title

      Organogenesis

      Volume: 6(4) Issue: 4 Pages: 234-44

    • DOI

      10.4161/org.6.4.12650

    • Related Report
      2011 Final Research Report
  • [Journal Article] Directed assembly of cell-Laden hydrogels for engineering functional tissues2010

    • Author(s)
      N.Kachouie, Y.Du, H.Bae, M.Khabiry, A.F.Ahari, B.Zamanian, J.Fukuda, A.Khademhosseini
    • Journal Title

      Organogenesis

      Volume: 6(4) Pages: 234-44

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 両性イオンペプチド分子層の還元脱離を利用した細胞組織の構築2012

    • Author(s)
      福田淳二、掛川貴弘、望月直人、大崎達哉、鈴木博章
    • Organizer
      電気化学会第79回大会
    • Place of Presentation
      アクトシティ浜松
    • Year and Date
      2012-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 両性イオンペプチド分子層の還元脱離を利用した細胞組織の構築2012

    • Author(s)
      福田淳二、掛川貴弘、望月直人、大崎達哉、鈴木博章
    • Organizer
      電気化学会第79回大会
    • Place of Presentation
      アクトシティ浜松(浜松市)
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] Surface Engineering for Rapid Cell Detachment by Using Electrochemical Desorption of a Zwitterionic Oligopeptide Layer2011

    • Author(s)
      T. Kakegawa, H. Suzuki, J. Fukuda
    • Organizer
      MRS Fall Meeting & Exhibit 2011
    • Place of Presentation
      Boston, USA
    • Year and Date
      2011-12-01
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 電気化学的な細胞脱離技術を用いたティッシュエンジニアリング2011

    • Author(s)
      望月直人、大崎達哉、掛川貴弘、鈴木博章、福田淳二
    • Organizer
      第49回日本人工臓器学会大会
    • Place of Presentation
      都市センターホテル
    • Year and Date
      2011-11-26
    • Related Report
      2011 Final Research Report
  • [Presentation] 電気化学的な細胞脱離技術を用いたティッシュエンジニアリング2011

    • Author(s)
      望月直人、大崎達哉、掛川貴弘、鈴木博章、福田淳二
    • Organizer
      第49回日本人工臓器学会大会
    • Place of Presentation
      都市センターホテル(東京都)
    • Year and Date
      2011-11-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 電気化学的な細胞脱離を利用した細胞シート回収技術2011

    • Author(s)
      望月直人、鈴木博章、福田淳二
    • Organizer
      第33回日本バイオマテリアル学会大会
    • Place of Presentation
      京都府民総合交流プラザ
    • Year and Date
      2011-11-02
    • Related Report
      2011 Final Research Report
  • [Presentation] 自己組織化単分子膜の電気化学還元脱離を用いた細胞脱離技術2011

    • Author(s)
      福田淳二、望月直人、掛川貴弘、鈴木博章
    • Organizer
      第33回日本バイオマテリアル学会大会
    • Place of Presentation
      京都府民総合交流プラザ
    • Year and Date
      2011-11-02
    • Related Report
      2011 Final Research Report
  • [Presentation] 電気化学的な細胞脱離を利用した細胞シート回収技術2011

    • Author(s)
      望月直人、鈴木博章、福田淳二
    • Organizer
      第33回日本バイオマテリアル学会大会
    • Place of Presentation
      京都府民総合交流プラザ(京都府)
    • Year and Date
      2011-11-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] 自己組織化単分子膜の電気化学還元脱離を用いた細胞脱離技術2011

    • Author(s)
      福田淳二、望月直人、掛川貴弘、鈴木博章
    • Organizer
      第33回日本バイオマテリアル学会大会
    • Place of Presentation
      京都府民総合交流プラザ(京都府)
    • Year and Date
      2011-11-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] Cell Detachment along with Electrical Cleavageof a Zwitterionic Oligopeptide Layer2011

    • Author(s)
      T. Kakegawa, H. Suzuki, J. Fukuda
    • Organizer
      Biofabrication 2011
    • Place of Presentation
      富山国際会議場
    • Year and Date
      2011-10-08
    • Related Report
      2011 Final Research Report
  • [Presentation] Microfluidic system for mastitic diagnosis based on superoxide secretion activity of neutrophils2011

    • Author(s)
      S. Kimura, J. Fukuda, A. Tajima, H. Suzuki
    • Organizer
      Biofabrication 2011
    • Place of Presentation
      富山国際会議場
    • Year and Date
      2011-10-08
    • Related Report
      2011 Final Research Report
  • [Presentation] Cell Detachment along with Electrical Cleavage of a Zwitterionic Oligopeptide Layer2011

    • Author(s)
      T.Kakegawa, H.Suzuki, J.Fukuda
    • Organizer
      Biofabrication 2011
    • Place of Presentation
      富山国際会議場(富山市)
    • Year and Date
      2011-10-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Microfluidic system for mastitic diagnosis based on superoxide secretion activity of neutrophils2011

    • Author(s)
      S.Kimura, J.Fukuda, A.Tajima, H.Suzuki
    • Organizer
      Biofabrication 2011
    • Place of Presentation
      富山国際会議場(富山市)
    • Year and Date
      2011-10-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] 細胞遊走試験のためのトランスフェクションアレイチップ2011

    • Author(s)
      榎本詢子、藤田聡史、長崎玲子、鈴木博章、福田淳二
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-09-28
    • Related Report
      2011 Final Research Report
  • [Presentation] 微細加工技術を利用した細胞培養チップ2011

    • Author(s)
      福田淳二
    • Organizer
      第63回日本生物工学会大会(招待講演)
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-09-28
    • Related Report
      2011 Final Research Report
  • [Presentation] 細胞遊走試験のためのトランスフェクションアレイチップ2011

    • Author(s)
      榎本詢子、藤田聡史、長崎玲子、鈴木博章、福田淳二
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学(府中市)
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 微細加工技術を利用した細胞培養チップ2011

    • Author(s)
      福田淳二
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学(府中市)(招待講演)
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] On-demand Detachment of Single Cells Using Microarrayed ITO Electrodes2011

    • Author(s)
      J Fukuda, Y Kameoka, H Suzuki
    • Organizer
      Asian Biomaterials Congress
    • Place of Presentation
      Busan, Korea
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 光架橋性ハイドロゲルを用いた血管網構築の試み2011

    • Author(s)
      大崎達哉、鈴木博章、福田淳二
    • Organizer
      化学工学会第43回秋季大会
    • Place of Presentation
      名古屋工業大学
    • Year and Date
      2011-09-14
    • Related Report
      2011 Final Research Report
  • [Presentation] 光架橋性ハイドロゲルを用いた血管網構築の試み2011

    • Author(s)
      大崎達哉、鈴木博章、福田淳二
    • Organizer
      化学工学会第43回秋季大会
    • Place of Presentation
      名古屋工業大学(名古屋市)
    • Year and Date
      2011-09-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Electrical Detachment of cells forEngineering Capillary-Like Structures in a Photocrosslinkable Hydrogel2011

    • Author(s)
      T. Osaki, T. Kakegawa, H. Suzuki & J. Fukuda
    • Organizer
      International Conference of the IEEE Engineering in Medicine and Biology Society
    • Place of Presentation
      Boston, USA
    • Year and Date
      2011-09-01
    • Related Report
      2011 Final Research Report
  • [Presentation] Electrical Detachment of cells for Engineering Capillary-Like Structures in a Photocrosslinkable Hydrogel2011

    • Author(s)
      T.Osaki, T.Kakegawa, H.Suzuki, J.Fukuda
    • Organizer
      International Conference of the IEEE Engineering in Medicine and Biology Society
    • Place of Presentation
      Boston, USA
    • Year and Date
      2011-09-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] Electrical Detachment of cells for Engineering Capillary-Like Structures in a Photocrosslinkable Hydrogel2011

    • Author(s)
      J Fukuda, Y Kameoka, H Suzuki
    • Organizer
      TERMIS-AP 2011
    • Place of Presentation
      Singapore, Singapore
    • Year and Date
      2011-08-04
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Spatio-Temporal Cell Detachment from Culture Surface Using Microarrayed Transparent Electrodes2011

    • Author(s)
      T. Osaki, T. Kakegawa, N. Sadr, A.Khademhosseini, H. Suzuki & J. Fukuda
    • Organizer
      TERMIS-AP 2011
    • Place of Presentation
      Singapore, Singapore
    • Year and Date
      2011-08-04
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 自己組織化単分子膜の還元脱離を利用した細胞組織体の構築2011

    • Author(s)
      福田淳二,掛川貴弘,望月直人,大崎達哉,鈴木博章
    • Organizer
      電気化学会第78回大会
    • Place of Presentation
      横浜国立大学
    • Year and Date
      2011-03-31
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 電気化学細胞脱離を利用した血管様構築の作製技術2011

    • Author(s)
      福田淳二、大崎達哉、掛川貴弘、望月直人、鈴木博章
    • Organizer
      第10回日本再生医療学会総会
    • Place of Presentation
      京王プラザホテル
    • Year and Date
      2011-03-02
    • Related Report
      2011 Final Research Report
  • [Presentation] 電気化学細胞脱離を利用したティッシュエンジニアリング2011

    • Author(s)
      福田淳二
    • Organizer
      日本動物実験代替法学会第23回大会
    • Place of Presentation
      北里大学
    • Year and Date
      2011-03-02
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 電気化学細胞脱離を利用した血管様構築の作製技術2011

    • Author(s)
      福田淳二、大崎達哉、掛川貴弘、望月直人、鈴木博章
    • Organizer
      第10回日本再生医療学会総会
    • Place of Presentation
      京王プラザホテル(新宿)
    • Year and Date
      2011-03-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] Fabrication of Thick Cell Sheet via Electrochemical Reactions on Porous Membrane Culture Substrate2010

    • Author(s)
      N. Mochizuki, H. Suzuki, J. Fukuda
    • Organizer
      2010 MRS Fall meeting
    • Place of Presentation
      Boston, USA
    • Year and Date
      2010-11-29
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 電気化学的原理に基づく細胞シート回収技術2010

    • Author(s)
      望月直人、鈴木博章、福田淳二
    • Organizer
      第62回日本生物工学会
    • Place of Presentation
      宮崎シーガイア
    • Year and Date
      2010-10-29
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Fabrication of Thick Cell Sheet via Electrochemical Reactions on Porous Membrane Culture Substrate2010

    • Author(s)
      N. Mochizuki, H. Suzuki, J. Fukuda
    • Organizer
      TERMIS-AP 2010
    • Place of Presentation
      Sydney, Australia
    • Year and Date
      2010-09-17
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Cell Detachment Along with Electrical Cleavage of a Zwitterionic Oligopeptide Layer2010

    • Author(s)
      T. Kakegawa, H. Suzuki, J. Fukuda
    • Organizer
      TERMIS-AP 2010
    • Place of Presentation
      Sydney, Australia
    • Year and Date
      2010-09-17
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 自己組織化オリゴペプチドを用いた細胞接着制御2010

    • Author(s)
      掛川貴弘、鈴木博章、福田淳二
    • Organizer
      化学工学会第42回秋季大会
    • Place of Presentation
      同志社大学
    • Year and Date
      2010-09-08
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] ITO電極を用いた電気化学的原理に基づく細胞脱離2010

    • Author(s)
      亀岡典哲、鈴木博章、福田淳二
    • Organizer
      化学工学会第42回秋季大会
    • Place of Presentation
      同志社大学
    • Year and Date
      2010-09-08
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.ims.tsukuba.ac.jp/~szk_fkd_lab/index.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.ims.tsukuba.ac.jp/~szk_fkd_lab/index.html

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] Cell culture method and device2011

    • Inventor(s)
      J Fukuda, H Suzuki, N Mochizuki, TKakegawa
    • Industrial Property Rights Holder
      University of Tsukuba
    • Filing Date
      2011-08-09
    • Related Report
      2011 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] Cell culture method and device2011

    • Inventor(s)
      J Fukuda, H Suzuki, N Mochizuki, T Kakegawa
    • Industrial Property Rights Holder
      University of Tsukuba
    • Filing Date
      2011-08-09
    • Related Report
      2011 Annual Research Report
    • Overseas

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Published: 2010-08-23   Modified: 2016-04-21  

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