Development of a cell microarray chip for evaluating invasion of cancer cells by transfer printing of transfected cells

• Project/Area Number: 22656189
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: The University of Tokyo
• Principal Investigator: NAGAMUNE Teruyuki 東京大学, 大学院・工学系研究科, 教授 (20124373)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,560,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: 細胞マイクロアレイ / 癌細胞 / 浸潤 / 細胞転写 / PEG脂質 / コラーゲンシート / 癌 / 細胞アレイ / 機能性核酸 / MMP / FRET

Research Abstract

We developed a method that can print a microarray of transfected cells from a glass substrate onto a hydrogel, and by using this method, microarrays of transfected cancer cells were successfully prepared in a three-dimensional extracellular matrix gel and employed for evaluating the anti-invasion activities of siRNA under an in vivo-like environment.

Research Products

• [Journal Article] Cell transfer printing from patterned poly(ethylene glycol)-oleyl surfaces to biological hydrogels for rapid and efficient cell micropatterning (2011)
  • Author(s): T. Takano, S. Yamaguchi, E. Matsunuma, S. Komiya, M. Shinkai, T. Takezawa and T. Nagamune
  • Journal Title: Biotechnol. Bioeng. Volume: 109 Pages: 244-251
  • Peer Reviewed
• [Journal Article] Cell transfer printing from patterned poly (ethylene glycol)-oleyl surfaces to biological hydrogels for rapid and efficient cell micropatterning (2011)
  • Author(s): T.Takano, et al
  • Journal Title: Biotechnology and Bioengineering Volume: 109 Issue: 1 Pages: 244-251
  • DOI: 10.1002/bit.23284
  • Peer Reviewed
• [Presentation] 癌浸潤性評価のためのゲル包埋型siRNAスクリーニング細胞マイクロアレイの開発 (2012)
  • Author(s): 小宮世乃吏
  • Organizer: 化学工学会第77年会
  • Place of Presentation: 工学院大学、東京
  • Year and Date: 2012-03-15
• [Presentation] 癌浸潤性評価のためのゲル包埋型siRNAスクリーニング細胞マイクロアレイの開発 (2012)
  • Author(s): 小宮世乃吏,山口哲志,三宅淳,河原正浩,長棟輝行
  • Organizer: 化学工学会第77年会
  • Place of Presentation: 東京,工学院大学
• [Presentation] PEG脂質系細胞修飾剤を用いた細胞アレイ技術 (2011)
  • Author(s): 山口哲志
  • Organizer: 第2回細胞アッセイ研究会
  • Place of Presentation: 東レ研修センター(三島)
  • Year and Date: 2011-01-28
• [Presentation] PEG脂質系細胞修飾剤を用いた細胞アレイ技術 (2011)
  • Author(s): 山口哲志,長棟輝行
  • Organizer: 第二回細胞アッセイ研究会
  • Place of Presentation: 三島,東レ研修センター
• [Presentation] Cell Micro-Patterning with Photo-Cleavable Anchor for Cell Membrane (2011)
  • Author(s): S. Yamaguchi, S. Yamahira, T. Nagamune
  • Organizer: Asian Congress on Biotechnology(ACB-2011)
  • Place of Presentation: Shanghai, China
• [Presentation] PEG脂質を用いた次世代細胞マイクロアレイの開発 (2011)
  • Author(s): 山口哲志,長棟輝行
  • Organizer: 第4回ナノバイオ若手ネットワーキングシンポジウム
  • Place of Presentation: 札幌,北海道大学フラテ会館
• [Book] Cell-Based Microarrays, Methods in Molecular Biology 706 (2011)
  • Author(s): Yamaguchi S
  • Total Pages: 8
  • Publisher: Humana Press
• [Book] Immobilized Culture and Tranfection Microarray of Non-adherent Cells, Cell-based microarrays, Methods in Molecular Biology 706(E. Palmer ed.) (2010)
  • Author(s): S. Yamaguchi, E. Matsunuma, and T. Nagamune
  • Publisher: Human Press.