Novel Onsite Transformation of Plant by Direct DNA Introduction
| Project/Area Number |
22656192
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Sojo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Shinjiro 崇城大学, 生物生命学部, 准教授 (40262307)
HAYASHI Shuhei 崇城大学, 生物生命学部, 助教 (30389522)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,290,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | オンサイト形質転換 / タバコ / 導管ネットワーク / 成長点 |
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| Research Abstract |
Availability of newly developed onsite transformation of mature plant by direct DNA introduction into every cell via vessel network was investigated. Firstly, transportation of a variety of materials via the vessel network was examined. Tobacco was used as a model plant in the present research. It was found that the materials in the vessel were rapidly transported independently of their molecular sizes. Introduction of a plasmid pBI121 harboring beta-glucuronidase(GUS) as a reporter gene into the tobacco for long term gave its GUS expression, suggesting the possibility of the formation of the transgenic tobacco. However, GUS expression in the control was occasionally detected, indicating that the analytic method of the GUS expression should be discussed.
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Report
(3 results)
Research Products
(5 results)
