• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of new determinants for double fertilization

Research Project

Project/Area Number 22657017
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Morphology/Structure
Research InstitutionChiba University (2011-2012)
The Institute of Physical and Chemical Research (2010)

Principal Investigator

IGAWA Tomoko  千葉大学, 大学院・園芸学研究科, 助教 (00360488)

Research Collaborator YAMADA Lixy  名古屋大学, 大学院・理学研究科, 助教 (10551020)
MORI Toshiyuki  早稲田大学, 高等研究所, 助教 (00462739)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,580,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywords重複受精 / 配偶子細胞 / ライブイメージング / 配偶子膜 / 卵細胞膜
Research Abstract

Arabidopsis marker lines, where gamete membrane was specifically visualized with fluorescent protein (GFP), were produced. Membrane proteins were purified from pistil tissue of the egg cell membrane- marker line by pull-down strategy using anti-GFP antibody. Among the identified proteins with putative trans-membrane region, one protein was found to be involved in seed fertility.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report

Research Products

(4 results)

All 2013 2012

All Journal Article Presentation

  • [Journal Article] Analysis of gamete membrane dynamics during double fertilization of Arabidopsis.2013

    • Author(s)
      Tomoko Igawa, Yuki Yanagawa, Shin-ya Miyagishima and Toshiyuki Mori
    • Journal Title

      Journal of Plant Research

      Volume: 126巻 Pages: 387-394

    • DOI

      10.1007/s10265-012-0528-0

    • NAID

      10031169281

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gamete membrane dynamics during double fertilization in Arabidopsis2013

    • Author(s)
      Tomoko Igawa, Toshiyuki Mori
    • Journal Title

      Plant Signaling Behavior

      Volume: 8巻in press

    • URL

      http://www.landesbioscience.com/journals/psb/article/24512/

    • Related Report
      2012 Final Research Report
  • [Presentation] Live cell imaging analysis of gamete membrane behavior during double fertilization2012

    • Author(s)
      Tomoko Igawa, Toshiyuki Mori
    • Organizer
      International Symposium on the Mechanisms of sexual Reproduction in Animals and Plants
    • Place of Presentation
      Hotel Nagoya Garden Palace
    • Related Report
      2012 Final Research Report
  • [Presentation] Live cell imaging analysis of gamete membrane behavior during double fertilization of Arabidopsis2012

    • Author(s)
      Tomoko Igawa
    • Organizer
      International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
    • Place of Presentation
      Hotel Nagoya Garden Palace
    • Related Report
      2012 Annual Research Report

URL: 

Published: 2010-08-22   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi