Control of behavior and locomotion by brain melanopsin

• Project/Area Number: 22657023
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Animal physiology/Animal behavior
• Research Institution: Konan University
• Principal Investigator: KUSAKABE Takehiro 甲南大学, 理工学部, 教授 (40280862)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,420,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: 脳・神経 / オプシン / 眼外光受容 / ホヤ / メダカ / 光受容

Research Abstract

Swimming behavior of the ascidian larva is controlled by photoreception of the ocellus. We have found that, however, the otx knockdown(morphant) larva, which completely lacks the ocellus, shows a photo response. By the analysis of expression sites and role of opsins other than the one expressed in the ocellus photoreceptor, we have identified melanopsin ortholog as a candidate opsin responsible for photo response in the otx morphant larva. We also found that a melanopsin gene is expressed in particular types of neurons in the brain of medaka Oryzias latipes.

Research Products

• [Journal Article] Cell lineage and cis-regulation for a unique GABAergic/glycinergic neuron type in the larval nerve cord of the ascidian Ciona intestinalis (2012)
  • Author(s): K.Nishitsuii
  • Journal Title: Development, Growth and Differentiation Volume: 54 Issue: 2 Pages: 177-186
  • DOI: 10.1111/j.1440-169x.2011.01319.x
  • Peer Reviewed
• [Journal Article] Monoaminergic modulation of photoreception in ascidian : Evidence for a proto-hypothalamo-retinal territory (2012)
  • Author(s): Florian Razy-Krajka, Euan R. Brown, Takeo Horie, Jacques Callebert, Yasunori Sasakura, Jean-Stéphane Joly, Takehiro G. Kusakabe, and Philippe Vernier
  • Journal Title: BMC Biology Volume: 10 Issue: 1 Pages: 45-45
  • DOI: 10.1186/1741-7007-10-45
  • Peer Reviewed
• [Journal Article] Ependymal cells of chordate larvae are stem-like cells from the adult nervous system (2011)
  • Author(s): Horie, T., Shinki, R., Ogura, Y., Kusakabe, T.G., Satoh, N., and Sasakura, Y.
  • Journal Title: Nature Volume: 469 Issue: 7331 Pages: 525-528
  • DOI: 10.1038/nature09631
  • Peer Reviewed
• [Journal Article] Ependymal cells of chordate larvae are stem-like cells that form the adult nervous system (2011)
  • Author(s): Takeo Horie
  • Journal Title: Nature Volume: 469 Pages: 525-528
  • Peer Reviewed
• [Journal Article] The ANISEED database : digital representation, formalization, and elucidation of a chordate developmental program (2010)
  • Author(s): O. Tassy, D. Dauga, F. Daian, D. Sobral, F. Robin, P. Khoueiry, D. Salgado, V Fox, D. Caillol, R. Schiappa, B. Laporte, A. Rios, G. Luxardi, T. Kusakabe, J. S. Joly, S. Darras, L. Christiaen, M. Contensin, H. Auger, C. Lamy, C. Hudson, U. Rothbacher, M. J. Gilchrist, K. W. Makabe, K. Hotta, S. Fujiwara, N. Satoh, Y Satou and P Lemaire
  • Journal Title: Genome Res Volume: 20 Issue: 10 Pages: 1459-1468
  • DOI: 10.1101/gr.108175.110
  • Peer Reviewed
• [Journal Article] The ANISEED database : Digital representation, formalization, and elucidation of a chordate developmental program (2010)
  • Author(s): Olivier Tassy
  • Journal Title: Genome Research Volume: 20 Pages: 1459-1468
  • Peer Reviewed
• [Presentation] Regulation and specification mechanisms of glutamatergic neurons during development of the larval nervous system of the ascidian Ciona intestinalis (2011)
  • Author(s): Koki Nishitsuji
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] ホヤ幼生のGABA/グリシン作動性神経細胞の細胞系譜と細胞特異的転写制御機構の解析 (2011)
  • Author(s): 西辻光希
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川市大雪アリーナ
  • Year and Date: 2011-09-22
• [Presentation] カタユウレイボヤ幼生の眼外光受容細胞 (2011)
  • Author(s): 日下部岳広
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川市大雪アリーナ
  • Year and Date: 2011-09-21
• [Presentation] ホヤ幼生のGABA/グリシン作動性神経細胞形成における転写因子SoxB1の役割 (2011)
  • Author(s): 一瀬葵
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川市大雪アリーナ
  • Year and Date: 2011-09-21
• [Presentation] Are there GnRH-producing cells in the hindbrain and the spinal cord of vertebrates? (2011)
  • Author(s): Toru Takigawa
  • Organizer: 第17回小型魚類研究会
  • Place of Presentation: 東レ総合研修センター
  • Year and Date: 2011-09-08
• [Presentation] 脊索動物に脊椎動物の起源を探る (2011)
  • Author(s): 日下部岳広
  • Organizer: 日本進化学会第13回大会
  • Place of Presentation: 京都大学百年記念ホール
  • Year and Date: 2011-07-31
• [Presentation] 脊索動物に脊椎動物の起源を探る (2011)
  • Author(s): 日下部岳広
  • Organizer: 日本進化学会第13回大会
  • Place of Presentation: 京都大学百年記念ホール(招待講演)
  • Year and Date: 2011-07-31
• [Presentation] The tunicate nervous system : insights into the origin of vertebrate-specific traits (2011)
  • Author(s): Takehiro G. Kusakabe
  • Organizer: 第8回国際比較生理生化学会議
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-06-05
• [Presentation] The tunicate nervous system : insights into the origin of vertebrate-specifie traits (2011)
  • Author(s): Takehiro G.Kusakabe
  • Organizer: 第8回国際比較生理生化学会議
  • Place of Presentation: 名古屋国際会議場(招待講演)
  • Year and Date: 2011-06-05
• [Presentation] 神経系の発生と多様性:脊索動物モデル・ホヤから脊椎動物モデル・メダカへの展開 (2011)
  • Author(s): 日下部岳広
  • Organizer: 第9回関西小型魚類勉強会
  • Place of Presentation: 産業総合技術研究所、尼崎(招待講演)
  • Year and Date: 2011-05-12
• [Presentation] Transcriptional regulation underlying the differentiation of glutamatergic neurons in the Ciona inteatinalis larva (2011)
  • Author(s): Aoi Ichinose
  • Organizer: International Symposium Genetic Eegulation of Development
  • Place of Presentation: 京都ガーデンパレス
• [Presentation] The origin and evolution of the cranial sensory organs and pituitary : evidence from basal chordates (2010)
  • Author(s): Takehiro G. Kusakabe
  • Organizer: 日本進化学会第12回東京大会・国際ワークショップ
  • Place of Presentation: 東京工業大学大岡山キャンパス
  • Year and Date: 2010-08-04
• [Presentation] The origin and evolution of the cranial sensory organs and pituitary : evidence from basal chordates (2010)
  • Author(s): Takehiro G.Kusakabe
  • Organizer: 日本進化学会第12回東京大会・国際ワークショップ
  • Place of Presentation: 東京工業大学大岡山キャンパス(招待講演)
  • Year and Date: 2010-08-04
• [Remarks] ホヤ遺伝子制御データベース(DBTGR)
  • URL: http://dbtgr.hgc.jp/
• [Remarks] ホヤ統合データベース(Aniseed)
  • URL: http://crfb.univ-mrs.fr/aniseed/