| Project/Area Number |
22657024
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | University of Tsukuba |
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Principal Investigator |
INOUYE Isao 筑波大学, 生命環境系, 教授 (70168433)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYAMA Takeshi 筑波大学, 生命環境系, 講師 (40302369)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,280,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | ゲノム / 細胞内共生 / 進化 |
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| Research Abstract |
Hatena arenicola, a kathablepharid flagellate that maintains endosymbiotic algae temporary, is considered to be in the intermediate stage of secondary endosymbiosis. However, it is difficult to get an enough amount of DNA from H. arenicola because the flagellate cannot be cultured. Thus, we study the possibility of the genomic analyses using DNA amplified from single or a few cells. We succeeded to get genomic DNA sequences from plural cells of H. arenicola, but the most reads (about 90%) consisted of 2. 4 base pair repeated sequences. We performed BLASTx for the remaining 10% reads and identified possible sequences originated from Hatena genome. However, it was difficult to cover the whole nuclear genome of Hatena using these sequences, thus we concluded that further examination is necessary.
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