Novel biflagellate TKB055 as a possible early-branch in the global eukaryotic phylogeny : studies on its morphology, transcriptome, and mitochondrial genome
| Project/Area Number |
22657025
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | University of Tsukuba |
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Principal Investigator |
INAGAKI Yuji 筑波大学, 生命環境系, 准教授 (50387958)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,420,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | ミトコンドリア / 真核生物系統 / 原始真核生物 / 細胞内微細構造 / EST解析 |
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| Research Abstract |
Discoba (Excavata) is a unicellular eukaryotic assemblage that comprises four subgroups: Heterolobosea, Euglenozoa, Jakobida and Tsukubamonadida. This clade is especially important for understanding the early evolution of mitochondrial (mt) genomes, as the jakobid Reclinomonas americana retains the most ancestral (i.e. gene-rich) genome of any mt genomes determined to date. Amongst discobids, the Tsukubamonadida is the most recently established group and contains a single representative species (Tsukubamonas globosa; formerly known as the biflagellate strain TKB055) whose precise phylogenetic position within the Discoba has not yet been resolved. We conducted a pyrosequencing-based transcriptomic survey of T globosa and, based on these data, we assembled a phylogenomic alignment of 157 proteins. Our phylogenomic analyses show that T. globosa branches at the base of the union of Euglenozoa and Heterolobosea. In addition, we completely sequenced the mt genome of T. globosa (48,463 bp in length and A+T content of 66.2%). The mt genome of T. globosa can be mapped as circular, and encodes 52 putative protein-coding and 29 RNA genes, indicating that T. globosa is second only to R. americana in terms of its coding capacity. The relationships amongst the majormembers of the Discoba clade inferred from the 157-protein phylogeny and their mt genome data enalbe us to elucidate the evolution of mt gene repertoires in this protist clade.
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Report
(3 results)
Research Products
(63 results)
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[Journal Article] A wide diversity of previously undetected relatives of diplomonads isolated from marine/saline habitats2010
Author(s)
Kolisko M, Silberman JD, Cepicka I, Yubuki N, Takishita K, Yabuki A, Leander BS, Inouye I, Inagaki Y, Roger AJ, Simpson AGB
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Journal Title
Environmental Microbiology
Volume: 12
Pages: 2700-2710
Related Report
Peer Reviewed
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[Journal Article] Comprehensive SSU rRNA phylogeny of Eukaryota2010
Author(s)
Ishida K, Inagaki Y, Sakaguchi M, Oiwa A, Kai A, Suzuki M, Nakayama T, Chikuni T, Yabuki A, Yamaguchi H, Yubuki N, Yoshida M, Nakayama T, Inouye I, Hashimoto T
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Journal Title
Journal of Endocytobiosis and Cell Research
Volume: 20
Pages: 81-88
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Peer Reviewed
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[Presentation] Tsukubamonas globosa is a deep-branching member in the Discoba clade : Morphology, phylogenomics, and mitochondrial genome(国際・招待講演)2011
Author(s)
Akinori Yabuki, Ryoma Kamikawa, Martin Kolisko, Sohta A. Ishikawa, Ken-ichiro Ishida, Alastair G. B. Simpson, Andrew J. Roger, Yuji Inagaki
Organizer
International Union of Microbiological Societies 2011 Congress
Place of Presentation
北海道, 札幌, 札幌コンベンションセンター
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[Presentation] Multigene phylogenies of diverse Carpediemonas-like organisms identify the closest relatives of 'amitochondriate' diplomonads and retortamonads2010
Author(s)
Takishita K, Kolisko M, Komatsuzaki H, Yabuki A, Cepicka I, Smejkalova P, Silberman JD, Inagaki Y, Hashimoto T, Roger AJ, Simpson AGB
Organizer
ISEP XVIII
Place of Presentation
石川県・金沢・金沢県立美術館
Year and Date
2010-07-02
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