Development of the general tags for formation of hetero-complex
| Project/Area Number |
22657028
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
YAO Min 北海道大学, 大学院・先端生命科学研究院, 准教授 (40311518)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,520,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | X線結晶解析 / 蛋白質工学 / 遺伝子工学 / タグ / ヘテロ複合体 / 多量体形 / バイオインフォマティクス |
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| Research Abstract |
In order to develop the general tags, HA-tags which can control and promote the formation of a hetero complex, we first searched candidate peptides of the tag from the protein crystal structures(more than the 3A resolution) from PDB in silico. Finally two kinds of hetero-dimer tag(2HA-tag) were selected. We constructed model proteins with 2HA-tag fusion at the N or C terminal, and over-expressed them in Escherichia coli. The experiments of hetero-dimer formation are in progress.
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Report
(3 results)
Research Products
(8 results)
