| Budget Amount *help |
¥3,520,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Research Abstract |
In order to develop the general tags, HA-tags which can control and promote the formation of a hetero complex, we first searched candidate peptides of the tag from the protein crystal structures(more than the 3A resolution) from PDB in silico. Finally two kinds of hetero-dimer tag(2HA-tag) were selected. We constructed model proteins with 2HA-tag fusion at the N or C terminal, and over-expressed them in Escherichia coli. The experiments of hetero-dimer formation are in progress.
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