| Project/Area Number |
22657030
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
KIMATA Yukio 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (60263448)
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,420,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | 酵母 / 細胞小器官 / オルガネラ / 小胞体 / ストレス応答 / 蛋白質 / プロテオーム / ストレス / 微生物 / 細胞形態 / 変異体 / 生細胞観察 |
|---|
| Research Abstract |
Dysfunction of the endoplasmic reticulum (ER), namely ER stress, activates an ER-located transmembrane protein Ire1. In the present study, we have noticed interaction between Ire1 and an ER-located zinc-ion transporter Zrg17 in yeast cells. Zrg17 was highly phosphorylated in IRE1 cells but not in ire1Δcells cultured in ER-stress conditions. We propose that Ire1 activated through zinc deficiency directly phosphorylates and stabilizes Zrg17 for efficient incorporation of zinc ion into the ER.
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