| Project/Area Number |
22657035
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | Nara Institute of Science and Technology |
|---|
Principal Investigator |
NAKAHATA Yasukazu 奈良先端科学技術大学院大学, バイサイエンス研究科, 助教 (50390810)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,420,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,600,000 (Direct Cost: ¥1,600,000)
|
|---|
| Keywords | 生体エネルギー変換 / ケミカルバイオロジー / FRET / NAD^+ / エネルギー代謝 / ライブイメージング |
|---|
| Research Abstract |
It is impossible to measure the intracellular NAD^+level with spatiotemporal information, because conventional methods for NAD^+measurement use cell lysates. In this study, we sought to develop a NAD^+FRET probe to monitor intracellular NAD^+in a living cell. As results, we succeeded to mke the NAD^+FRET probe that might monitor intracellular NAD^+.
|
