| Project/Area Number |
22657036
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
MATSUNAGA Hayato 長崎大学, 大学院・医歯薬学総合研究科, 助教 (20437833)
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| Co-Investigator(Renkei-kenkyūsha) |
UEDA Hiroshi 長崎大学, 医歯(薬)学総合研究科, 教授 (00145674)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,330,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | 細胞情報伝達機構 / ストレス / 生理活性 / 脳・神経 / プロテオーム |
|---|
| Research Abstract |
The nuclear protein prothymosin-α(ProTα), which possesses multi-function is an intrinsic inhibitor of necrosis and apoptosis. We have already revealed that ProTαis released from neurons and astrocytes on ischemic stress through non-vesicular manner and exerts a unique form of neuroprotection through an anti-necrotic mechanism. Previously, we found out that released ProTαonce again makes the transition to the nucleus in some cells. In this study, we comprehensively identified the ProTα-binding proteins in process of extracellular release and re-nuclear localization of ProTα, and performed protein-protein interaction analysis in in vitro and cell-based assay. Finally, we succeeded the identification of non-vesicular release involved proteins, ProTα-binding proteins in the nucleus and novel cell membrane receptor. In addition, we simulated variation of machineries involved in self-defensive role of ProTαby use of Cell Illustrator software, and obtained the similar result of experimental work.
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