Analysis of the relationship between Chemotaxis system and rotational motion of motors of the bacteria by the multi-motor measurements
Project/Area Number |
22657038
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Biophysics
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Research Institution | Tohoku University |
Principal Investigator |
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Project Period (FY) |
2010 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥3,480,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥1,400,000 (Direct Cost: ¥1,400,000)
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Keywords | 1分計測 / 生物物理 / ナノバイオ / ナノマシン / 分子モーター / バクテリア / 回転計測 / モータータンパク / 温度 / トルク |
Research Abstract |
The bacteria flagellar motor is a motor of which diameter is only 40nm, and rotate about 1000 rotations per second using flow of ions. E. Coli has about 5 filaments and changes these directions by the information from chemo-receptors. Although, the bacteria has works with highly sophisticated signal transduction, energy conversion, and cooperation system, we have a few information about them. In this research, by fusing nano-measurements system and imaging system, the relationship between each motors, the response of the motors by the chemo-signals, the response of the motor by the external stimuli, the correlation between the local stimulation by the caged-compounds and the response of the cell and the correlation between the regulation of the gene expression an rotational motion of the motor. Using these techniques, we found that there were correlations of the switching of each motors, and this timing has some delay. This delay was depended on the position of the each motors, that suggested that the information from the cell pole propagated as wave. Using caged-compounds, the rotational directions of the motors, CW-bias were changed.
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Report
(3 results)
Research Products
(28 results)
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[Journal Article] Adhesion Effects of a Guanidinium Ion Appended Dendritic "Molecular Glue" on the ATP-Driven Sliding Motion of Actomyosin.2010
Author(s)
Okuro, K., Kinbara, K., Takeda, K., Inoue, Y., Ishijima, A., Aida, T.
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Journal Title
Angew Chem Int Ed Engl.
Volume: 49
Pages: 3030-3033
Related Report
Peer Reviewed
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[Journal Article] Thermosensing function of the Escherichia coli redox sensor Aer.2010
Author(s)
Nishiyama, S., Ohno, S., Ohta, N., Inoue, Y.Fukuoka, H., Ishijima, A., Kawagishi, I.
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Journal Title
J Bacteriol
Volume: 192
Pages: 1740-1743
Related Report
Peer Reviewed
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