| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Research Abstract |
We developed the method of exchanging a bacteria genome. In order to perform genome exchange, a host's genome must remove. So, we succeeded in preparation of the genome destructive digestion bacteria using a restriction enzyme expression system. Furthermore, we succeeded also in methylation of the genome to introduce and prepared the genome by which restriction enzyme is not cut. We tried to exchange of the genome using genome destruction bacteria and methylation genome. As a result, some colonies could be obtained. However, it was suggested as a result of the genome PCR or the sequence analysis of the genome that the genome of thesebacteria is a chimera of two genomes.
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