| Project/Area Number |
22657041
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
HOSOKAWA Yoichiroh 奈良先端科学技術大学院大学, 物質創成科学研究科, 特任准教授 (20448088)
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| Co-Investigator(Kenkyū-buntansha) |
ITO Akihiko 近畿大学, 医学部, 教授 (80273647)
FURUNO Tadahide 愛知学院大学, 薬学部, 准教授 (80254308)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,420,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | フェムト秒レーザー / 衝撃力 / 細胞 / カルシウムイオン / イメージング |
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| Research Abstract |
When an intense infrared femtosecond laser is focused into cell culture medium under microscope, an explosion is induced at the laser focal point through an efficient multi-photon absorption. Since the explosion localizes in the area with order of a few tens micron, a mechanical impulsive force can load on a single cultured animal cell. In this investigation, the femtosecond laser-induced impulsive force was loaded on a single muscle myoblast cells and neurons, and then modulation of the activity was investigated by calcium ion(Ca^<2+>) mobilization. An advantage of this stimulation method is that the force magnitude can flexibly control with the laser intensity and pulse repetition rate. The mechanism of the cell activity modulation by the mechanical stimulation was investigated by this new method.
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