| Project/Area Number |
22657044
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Molecular biology
|
|---|
| Research Institution | National Institute of Genetics |
|---|
Principal Investigator |
MAESHIMA Kazuhiro 国立遺伝学研究所, 構造遺伝学研究センター, 教授 (00392118)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TAKATA Hideaki 大阪大学, 工学(系)研究科(研究院), 助教 (20455207)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,300,000)
|
|---|
| Keywords | 核酸 / ゲノム / 生体分子 |
|---|
| Research Abstract |
To label a certain genomic region in the cell, a harsh condition to denature DNA is required. In this proposal, we tried to target a fluorescently tagged DNA sequence to the genomic DNA using some homologous recombination proteins. However, with homologous recombination protein RecA or XRad51, we did not get an efficient targeting in the fixed cells. As an alternative, we designed some fluorescent polyamides, which can recognize a certain DNA sequence, and succeeded to target efficiently the telomere repeats in the fixed cells
|
