| Project/Area Number |
22657047
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
HARADA Ichiro 東京工業大学, 大学院・生命理工学研究科, 助教 (00361759)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,060,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | 細胞間情報伝達 / 上皮増殖因子 / E-カドヘリン / 基質固定化型タンパク質 / 光反応タンパク質 |
|---|
| Research Abstract |
Numerous studies have shown that there are signal propagation between cells in a colony involved in the intercellular signaling. To analyze mechanism of the signaling propagation in the cell colony, we designed local stimulation technique using E-cadherin-immobilized micro-spheres or hydrogel, which gives local mechanical stimulation in a cell-sheet. We found that the local stimulation of E-cad-spheres to the periphery of the MDCK cell inhibit collective motion of the cell colony and inactivate ERK signaling, which propagated into the cell colony. Also, we found that local mechanical stimulation to the cell sheet induced ERK signaling to cells in a non-stimulated section suggested mechanical balance involved in actin network is one the transducer of signal propagation involved in collective cellular motion.
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