Screening of maternal-effect mutants by using Bloom-deficient medaka

• Project/Area Number: 22657055
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Nagoya University
• Principal Investigator: HIBI Masahiko 名古屋大学, 生物機能開発利用研究センター, 教授 (40273627)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,420,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: 発生遺伝 / 体軸形成 / 初期発生 / 胚葉形成 / 母性遺伝子産物 / 姉妹染色分体交換 / Bloom遺伝子 / 変異体 / メダカ / 姉妹染色分交換 / 変異体スクリー

Research Abstract

During early vertebrate embryogenesis, RNAs and proteins deposited in oocytes(maternal gene products) play essential roles in formation of germ layers and body axes. Bloom gene is involved in DNA repair and encodes a RecQ helicase. In Bloom-deficient cells, the sister chromatic exchange frequently takes place. In this study, we have developed a method to make maternal-effect mutants using Bloom-deficient medaka. We show that this method can be used to identify maternal genes that control the formation of the germ layers and body axes.

Research Products

• [Journal Article] Development and evolution of cerebellar neural circuits (2012)
  • Author(s): Hashimoto, M. and Hibi, M.
  • Journal Title: Dev. Growth Differ Volume: 54 Issue: 3 Pages: 373-389
  • DOI: 10.1111/j.1440-169x.2012.01348.x
  • NAID: 10031136910
• [Journal Article] Lesion-induced generation of interneuron cell types in specific dorso-ventral domains in the spinal cord of adult zebrafish (2012)
  • Author(s): Kuscha, V., Fraser, S. L., Dias, T. B., Hibi, M., Becker, T., and Becker, C. G.
  • Journal Title: J. Comp. Neurol Volume: (in press) Issue: 16 Pages: 3604-3616
  • DOI: 10.1002/cne.23115
  • Peer Reviewed
• [Journal Article] The Medaka zic1/zic4 Mutant Provides Molecular Insights into Teleost Caudal Fin Evolution (2012)
  • Author(s): Moriyama, Y., Kawanishi, T., Nakamura, R., Tsukahara, T., Sumiyama, K., Suster, M. L., Kawakami, K., Toyoda, A., Fujiyama, A., Yasuoka, Y., Nagao, Y., Sawatari, E., Shimizu, A., Wakamatsu, Y., Hibi, M., Taira, M., Okabe, M., Naruse, K., Hashimoto, H., Shimada, A., and Takeda, H.
  • Journal Title: Curr. Biol Volume: 22 Pages: 601-601
• [Journal Article] Dynamic changes in the gene expression of zebrafish Reelin receptors during embryogenesis and hatching period (2012)
  • Author(s): Hideaki Imai, Yoshihito Oomiya, Satoshi Kikkawa, Wataru Shoji, Masahiko Hibi, Toshio Terashima, Yu Katsuyama
  • Journal Title: Development, Growth & Differentiation Volume: Vol.54 Issue: 2 Pages: 253-263
  • DOI: 10.1111/j.1440-169x.2012.01327.x
  • Peer Reviewed
• [Journal Article] Development of the cerebellum and cerebellar neural circuits (2012)
  • Author(s): Hibi, M. and Shimizu T.
  • Journal Title: Dev. Neurobiol Volume: 72 Issue: 3 Pages: 282-301
  • DOI: 10.1002/dneu.20875
• [Journal Article] The medaka zic1/zic4 mutant provides molecular insights into teleost caudal fin evolution. (2012)
  • Author(s): Yuuta Moriyama
  • Journal Title: Current Biology Volume: 21 Issue: 7 Pages: 601-607
  • DOI: 10.1016/j.cub.2012.01.063
  • Peer Reviewed
• [Journal Article] Function of strawberry notch family genes in the zebrafish brain development (2011)
  • Author(s): Takano, A., Zochi, R., Hibi, M., Terashima, T., and Katsuyama, Y.
  • Journal Title: Kobe. J. Med Volume: 56
  • NAID: 110009587651
  • URL: http://www.med.kobe-u.ac.jp/journal/contents/56/E220.pdf
• [Journal Article] Development of the cerebellum and cerebellar neural circuit (2011)
  • Author(s): Hibi, M.
  • Journal Title: Developmental Neurobiology
  • Peer Reviewed
• [Journal Article] Atypical PKC regulates primary dendrite specification of cerebellar Purkinje cells by localizing Golgi apparatus (2010)
  • Author(s): Tanabe, K., Kani, S., Shimizu, T., Bae, Y.-K., Abe, T., and Hibi, M.
  • Journal Title: J. Neurosci Volume: 30 Issue: 50 Pages: 16983-16992
  • DOI: 10.1523/jneurosci.3352-10.2010
• [Journal Article] Dynein axonemal intermediate chain 2 is required for formation of the left-right body axis and kidney in medaka (2010)
  • Author(s): Nagao, Y., Cheng, J., Kamura, K., Seki, R., Maeda, A., Nihei, D., Koshida, S., Wakamatsu, Y., Fujimoto, T., Hibi, M., and Hashimoto, H.
  • Journal Title: Dev. Biol. Volume: 347 Issue: 1 Pages: 53-61
  • DOI: 10.1016/j.ydbio.2010.08.001
• [Journal Article] Expression of strawberry notch family genes during zebrafish embryogenesis (2010)
  • Author(s): Takano, A., Zochi, R., Hibi, M., Terashima, T., and Katsuyama, Y.
  • Journal Title: Dev. Dyn. Volume: 239 Issue: 6 Pages: 1789-1796
  • DOI: 10.1002/dvdy.22287
  • Peer Reviewed
• [Journal Article] Proneural gene-linked neurogenesis in zebrafish cerebellum (2010)
  • Author(s): Kani, S., Bae, Y.-K., Shimizu, T., Tanabe, K., Satou, C., Parsons, M. J. Scott, E., Higashijima, S.-I., and Hibi, M.
  • Journal Title: Dev. Biol. Volume: 343 Issue: 1-2 Pages: 1-17
  • DOI: 10.1016/j.ydbio.2010.03.024
• [Journal Article] Zinc-finger genes Fezfl and Fezfl control neuronal differentiation by repressing Hes5 expression in forebrain (2010)
  • Author(s): Shimizu, T., Nakazawa, M., Kani, S., Bae, Y.-K., Shimizu, T., Kageyama, R., and Hibi, M.
  • Journal Title: Development Volume: 137 Issue: 11 Pages: 1875-1885
  • DOI: 10.1242/dev.047167
• [Journal Article] Syntabulin a motor protein linker controls dorsal determination. (2010)
  • Author(s): Nojima, H., Rothhamel, S., Shimizu, T., Kim, C. H., Yonemura, S., Marlow, F. L., Hibi, M.
  • Journal Title: Development Volume: 137 Issue: 6 Pages: 923-933
  • DOI: 10.1242/dev.046425
• [Journal Article] Zinc finger genes Fezf1 and Fezf2 control neuronal differentiation by repressing Hes5 expression in forebrain (2010)
  • Author(s): Shimizu, T.
  • Journal Title: Development Volume: 137 Pages: 1875-1885
  • Peer Reviewed
• [Journal Article] Proneural gene-linked neurogenesis in zebrafish cerebellum (2010)
  • Author(s): Kani, S.
  • Journal Title: Developmental Biology Volume: 343 Pages: 1-17
  • Peer Reviewed
• [Journal Article] Expression of strawberry notch family genes during zebrafish embryogenesis (2010)
  • Author(s): Takano, A.
  • Journal Title: Developmental Dynamics Volume: 239 Pages: 1789-1796
  • Peer Reviewed
• [Journal Article] Dynein axonemal intermediate chain 2 is required for formation of the left-right body axis and kidney in medaka (2010)
  • Author(s): Nagao, Y.
  • Journal Title: Developmental Biology Volume: 347 Pages: 53-61
  • Peer Reviewed
• [Journal Article] Atypical PKC regulates primary dendrite specification of cerebellar Purkinje cells by localizing Golgi apparatus (2010)
  • Author(s): Tanabe, K.
  • Journal Title: Journal of Neuroscience Volume: 30 Pages: 16983-16992
  • Peer Reviewed
• [Presentation] Genetic control for formation of cerebellar neural circuits in zebrafish and medaka (2012)
  • Author(s): Hibi, M.
  • Organizer: GCOE International Symposium ; Formation and Function of Brain Networks : From Genes to Behaviors
  • Place of Presentation: 名古屋
  • Year and Date: 2012-01-12
• [Presentation] Signaling and gene cascades controlling embryonic polarity (2011)
  • Author(s): Hibi, M.
  • Organizer: Karin Lab Symposium
  • Place of Presentation: La Jolla, California, USA
  • Year and Date: 2011-11-19
• [Presentation] Development of cerebellar neural circuits in zebrafish and medaka (2011)
  • Author(s): Hibi, M.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-15
• [Presentation] Genetic control of cerebellar neural circuits in zebrafish and medaka (2011)
  • Author(s): Hibi, M.
  • Organizer: The 5^<th> Asia Oceania Zebrafish Meeting
  • Place of Presentation: 北京
  • Year and Date: 2011-08-27
• [Presentation] Microtubule-dependent dorsal determination in zebrafish (2011)
  • Author(s): Seki, R.
  • Organizer: 第44回日本発生生物学会年会
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-20
• [Presentation] Genetic Control of Cerebellar Neural Circuit Formation (2011)
  • Author(s): Hibi, M.
  • Organizer: 4^<th> Strategic Conference of Zebrafish Investigators
  • Place of Presentation: Asilomar, California, USA
• [Presentation] Dendrite formation of Purkinje cells in zebrafish cerebellum (2010)
  • Author(s): 日比正彦
  • Organizer: Neuro2010
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2010-09-04
• [Presentation] Excessive and Ectopic Development of Embryonic Leucophores in Medaka ml-3 Mutant is Caused by sox5 Mutation. (2010)
  • Author(s): Hashimoto, H.
  • Organizer: 8^<th> International Conference on Zebrfish Development and Genetics
  • Place of Presentation: Madison, Wisconsin, USA
• [Presentation] Syntabulin, a Motor Protein Linker, Controls Dorsal Determination (2010)
  • Author(s): Nojima, H.
  • Organizer: 8^<th> International Conference on Zebrfish Development and Genetics
  • Place of Presentation: Madison, Wisconsin, USA
• [Presentation] Dynein Axonemal Intermediate Chain 2 is Required for Formation of the Left-right Body Axis and Kidney in Medaka (2010)
  • Author(s): Nagao, H.
  • Organizer: 8^<th> International Conference on Zebrfish Development and Genetics
  • Place of Presentation: Madison, Wisconsin, USA
• [Presentation] Neurogenesis in Zebrafish Cerebellum (2010)
  • Author(s): Shimizu, T.
  • Organizer: 8^<th> International Conference on Zebrfish Development and Genetics
  • Place of Presentation: Madison, Wisconsin, USA