Development of assay systems involving epigenetic control of gene expression to identify novel functional components in plants

• Project/Area Number: 22658001
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Breeding science
• Research Institution: Hokkaido University
• Principal Investigator: KANAZAWA Akira 北海道大学, 大学院・農学研究院, 准教授 (30281794)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,480,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: 植物 / 機能性成分 / エピジェネティクス / シトシンメチル化 / 転写抑制

Research Abstract

In terms of finding novel functions of plant-derived compounds, assay systems for inhibitory activity of DNA methylation and transcriptional repression were developed using transgenic plants that carry an epigenetically silenced transgene. Through these assays, genistein, anisoflavonoid, was identified as the first plant-derived compound that had such an activity in plant cells.

Research Products

• [Journal Article] Deep sequencing uncovers commonality in small RNA profiles between transgene-induced and naturally occurring RNA silencing of chalcone synthase-A gene in petunia. (2013)
  • Author(s): Megumi Kasai
  • Journal Title: BMC Genomics Volume: 14 Issue: 1 Pages: 63-63
  • DOI: 10.1186/1471-2164-14-63
  • Peer Reviewed
• [Journal Article] Enhancement of RNA-directed DNA methylation of a transgene by simultaneously downregulating a ROS1 orthologue using a virus vector in Nicotiana benthamiana.Front (2013)
  • Author(s): Otagaki, S., Kasai, M., Masuta, C. and Kanazawa, A
  • Journal Title: Genet Volume: 4 Pages: 44-44
  • DOI: 10.3389/fgene.2013.00044
  • Peer Reviewed
• [Journal Article] Induction of RNA-directed DNA methylation and heritable transcriptional gene silencing as a tool to engineer novel traits in plants (2013)
  • Author(s): Kasai, M. and Kanazawa, A
  • Journal Title: Plant Biotechnol Volume: (in press)
  • NAID: 10031202041
  • Peer Reviewed
• [Journal Article] Induction of RNA-directed DNA methylation and heritable transcriptional gene silencing as a tool to engineer novel traits in plants. (2013)
  • Author(s): Megumi Kasai
  • Journal Title: Plant Biotechnol. Volume: in press
  • Peer Reviewed
• [Journal Article] Complex genetic nature of sex-independent transmission ratio distortion in Asian rice species: the involvement of unlinkedmodifiers and sex-specific mechanisms (2012)
  • Author(s): Koide, Y., Shinya, Y., Ikenaga, M., Sawamura, N., Matsubara, K., Onishi, K., Kanazawa, A. and Sano, Y
  • Journal Title: Heredity Volume: 108 Issue: 3 Pages: 242-247
  • DOI: 10.1038/hdy.2011.64
  • Peer Reviewed
• [Journal Article] Coincident sequence-specific RNA degradation of linked transgenes in the plant genome (2012)
  • Author(s): Kasai, M., Koseki, M., Goto, K., Masuta, C., Ishii, S., Hellens, R. P., Taneda, A. and Kanazawa, A
  • Journal Title: PlantMol. Biol Volume: 78 Issue: 3 Pages: 259-273
  • DOI: 10.1007/s11103-011-9863-0
  • Peer Reviewed
• [Journal Article] In planta assays involving epigenetically silenced genes reveal inhibition of cytosine methylation bygenistein (2012)
  • Author(s): Arase, S., Kasai, M. and Kanazawa, A
  • Journal Title: Plant Methods Volume: 8 Issue: 1 Pages: 10-10
  • DOI: 10.1186/1746-4811-8-10
  • Peer Reviewed
• [Journal Article] An active CACTA-family transposable element is responsible for flower variegation in wild soybean Glycine soja (2012)
  • Author(s): Takahashi, R., Morita, Y., Nakayama, M., Kanazawa, A. and Abe, J
  • Journal Title: Plant Genome Volume: 5 Issue: 2 Pages: 62-70
  • DOI: 10.3835/plantgenome2011.11.0028
  • Peer Reviewed
• [Journal Article] RNA silencing as a tool to uncover gene function and engineer novel traits in soybean (2012)
  • Author(s): Kasai, M. and Kanazawa, A
  • Journal Title: Breeding Science Volume: 61 Issue: 5 Pages: 468-479
  • DOI: 10.1270/jsbbs.61.468
  • NAID: 130004057128
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed
• [Journal Article] 植物改変の新技術,エピジェネティックな遺伝子発現制御 (2012)
  • Author(s): 金澤章、河西めぐみ
  • Journal Title: BIO INDUSTRY Volume: 29巻8号 Pages: 26-34
• [Journal Article] 植物改変の新技術：エピジェネティックな遺伝子発現制御 (2012)
  • Author(s): 金澤 章
  • Journal Title: BIO INDUSTRY Volume: 29 Pages: 26-34
• [Journal Article] Virus-mediated efficient induction of epigenetic modifications of endogenous genes with phenotypic changes in plants (2011)
  • Author(s): Kanazawa, A., Inaba, J., Shimura, H., Otagaki, S., Tsukahara, S., Matsuzawa, A., Kim, B. M., Goto, K. and Masuta, C.
  • Journal Title: Plant J Volume: 65 Issue: 1 Pages: 156-168
  • DOI: 10.1111/j.1365-313x.2010.04401.x
  • NAID: 120005946605
  • Peer Reviewed
• [Journal Article] Establishment of an in vitro culture and particle bombardment-mediated transformation system in Miscanthus sinensis Anderss., a potential bioenergy crop (2011)
  • Author(s): Wang, X., Yamada, T., Kong, F., Abe, Y., Hoshino, Y., Sato, H., Takamizo, T., Kanazawa, A. and Yamada, T.(2011)
  • Journal Title: Global Change Biology Bioenergy Volume: 3 Issue: 4 Pages: 322-332
  • DOI: 10.1111/j.1757-1707.2011.01090.x
  • Peer Reviewed
• [Journal Article] Optimization of ion-beam irradiation for mutagenesis in soybean: effects on plant growth and production of visibly altered mutants (2011)
  • Author(s): Arase, S., Hase, Y., Abe, J., Kasai, M., Yamada, T., Kitamura, K., Narumi, I., Tanaka, A. and Kanazawa, A
  • Journal Title: Plant Biotechnology Volume: 28 Issue: 3 Pages: 323-329
  • DOI: 10.5511/plantbiotechnology.11.0111a
  • NAID: 130000668812
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Journal Article] RNA-mediated epigenetic modifications of an endogenous gene targeted by a viral vector: a potent gene silencing system to produce a lant that does not carry a transgene but has altered traits (2011)
  • Author(s): Kanazawa, A., Inaba, J., Kasai, M., Shimura, H. and Masuta, C
  • Journal Title: Plant Signal. Behav Volume: 6 Issue: 8 Pages: 1090-1093
  • DOI: 10.4161/psb.6.8.16046
  • Peer Reviewed
• [Journal Article] Size and positional effects of promoter RNA segments on virus-induced RNA-directed DNA methylation and transcriptional genesilencing (2011)
  • Author(s): Otagaki, S., Kawai, M., Masuta, C. and Kanazawa, A
  • Journal Title: Epigenetics Volume: 6 Issue: 6 Pages: 681-691
  • DOI: 10.4161/epi.6.6.16214
  • Peer Reviewed
• [Journal Article] 外来遺伝子を持たずに特定形質が変化した植物をつくる育種法 (2011)
  • Author(s): 金澤章
  • Journal Title: ニューカントリー Volume: 58巻693号 Pages: 70-71
• [Journal Article] Soybean stem growth habit gene Dt1 is an orthologue of Arabidopsis TFL1 (2010)
  • Author(s): Liu, B., Watanabe, S., Uchimiya, T., Kong, F., Kanazawa, A., Xia, Z., Nagamatsu, A., Arai, M., Yamada, T., Kitamura, K., Masuta, C., Harada, K. and Abe, J
  • Journal Title: Plant Physiol Volume: 153 Issue: 1 Pages: 198-210
  • DOI: 10.1104/pp.109.150607
  • Peer Reviewed
• [Journal Article] Two coordinately regulated homologs of FLOWERING LOCUS T are involved in the control of photoperiodic flowering in soybean (2010)
  • Author(s): Kong, F., Liu, B., Xia, Z., Sato, S., Kim, B., Watanabe, S., Yamada, T., Tabata, S., Kanazawa, A., Harada, K. and Abe, J
  • Journal Title: Plant Physiol Volume: 154 Issue: 3 Pages: 1220-1231
  • DOI: 10.1104/pp.110.160796
  • Peer Reviewed
• [Journal Article] Fertility restoration by Ifr1 in rice with BT-type cytoplasmic male sterility is associated with a reduced level, but not processing of atp6-orf79 co-transcribed RNA (2010)
  • Author(s): Ohta, H., Ogino, A., Kasai, M., Sano, Y. and Kanazawa, A
  • Journal Title: Plant Cell Rep Volume: 29 Issue: 4 Pages: 359-369
  • DOI: 10.1007/s00299-010-0827-7
  • NAID: 120005946604
  • Peer Reviewed
• [Journal Article] Fertility restoration by lfr1 in rice with BT-type cytoplasmic male sterility is associated with a reduced level, but not processing, of atp6-orf79 co-transcribed RNA (2010)
  • Author(s): Haruka Ohta
  • Journal Title: Plant Cell Rep Volume: 29 Pages: 359-369
  • Peer Reviewed
• [Presentation] RNA-mediated epigenetic modifications by a viral vector:a potent system to produce a plant that does not carry a transgene but has altered traits (2012)
  • Author(s): Kanazawa, A., Inaba, J., Kasai, M., Shimura, H. and Masuta, C
  • Organizer: 10th International Congress on Plant Molecular Biology
  • Place of Presentation: ICC, Jeju, Republic of Korea
  • Year and Date: 2012-10-24
• [Presentation] Transcription and transposition of the soybean retrotransposon SORE-1 (2012)
  • Author(s): Tsuchiya, M., Yuan, H., Sato, M., Kasai, M., Abe, J. and Kanazawa, A
  • Organizer: 10th International Congress on Plant Molecular Biology.
  • Place of Presentation: ICC, Jeju, Republic of Korea
  • Year and Date: 2012-10-24
• [Presentation] Coincident RNA silencing of linked transgenes in the plant genome mediated by read-through transcription (2012)
  • Author(s): Kasai, M., Taneda, A. and Kanazawa, A
  • Organizer: 10th International Congress on Plant Molecular Biology
  • Place of Presentation: ICC, Jeju, Republic of Korea
  • Year and Date: 2012-10-23
• [Presentation] エピジェネティックな機構による転写抑制の解除を利用したinplantaアッセイ系により明らかになった植物成分のDNAメチル化抑制効果 (2012)
  • Author(s): 荒瀬幸子・河西めぐみ・金澤章
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 福岡市九州大学
  • Year and Date: 2012-09-25
• [Presentation] ディープシークエンス解析によって明らかになった、ペチュニアにおけるコサプレッションとnaturallyoccurringRNAサイレンシングの低分子RNA産生機構の共通性 (2012)
  • Author(s): 河西めぐみ・松村英生・吉田健太郎・寺内良平・種田晃人・金澤章
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 福岡市九州大学
  • Year and Date: 2012-09-25
• [Presentation] ペチュニア形質転換体における長期間の育成により誘導されたコサプレッションからの復帰 (2012)
  • Author(s): 河西めぐみ・金澤章
  • Organizer: 日本育種学会第122回講演会
  • Place of Presentation: 京都市京都産業大学
  • Year and Date: 2012-09-15
• [Presentation] エピジェネティックな遺伝子発現制御を介した形質改変 (2012)
  • Author(s): 金澤章・河西めぐみ
  • Organizer: 日本育種学会第54回シンポジウム「エピミュータジェネシスと次世代育種への展開」
  • Place of Presentation: 京都市京都産業大学
  • Year and Date: 2012-09-14
• [Presentation] RNA-mediated epigenetic modifications provide a tool to produce a plant that does not carry a transgene but has altered traits (2012)
  • Author(s): Akira Kanazawa
  • Organizer: Japan-Australia Symposium on Plant Science for Agriculture
  • Place of Presentation: Hokkaido Univ.,(札幌市)
  • Year and Date: 2012-01-19
• [Presentation] Introduction of fructosyltransferase genes of the cool season grass, Lolium perenne into the warm season grass, Miscanthus sinensis, a potential bioenergy crop. (2012)
  • Author(s): Toshihiko Yamada
  • Organizer: International Plant & Animal Genome Conference
  • Place of Presentation: The Town & Country Hotel, San Diego, (USA)
  • Year and Date: 2012-01-14
• [Presentation] ダイズのレトロトランスポゾンSORE-1の組織特異的プロモーター活性 (2011)
  • Author(s): 土屋真弓
  • Organizer: 札幌農林学会平成23年度大会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-12-03
• [Presentation] RNA-mediated epigenetic modifications of an endogenous gene as a tool to produce a plant that does not carry a transgene but has altered traits (2011)
  • Author(s): Kanazawa, A., Inaba, J., Kasai, M., Shimura, H. and Masuta, C
  • Organizer: SOL&ICuGI2011 8th Solanaceae and 2nd CucurbitaceaeGenome Joint Conference
  • Place of Presentation: Kobe ConventionCenter, Kobe, Japan
  • Year and Date: 2011-12-01
• [Presentation] RNA-mediated epigenetic modifications of an endogenous gene as a tool to produce a plant that does not carry a transgene but has altered traits (2011)
  • Author(s): Akira Kanazawa
  • Organizer: SOL&ICuGI 2011 8^<th> Solanaceae and 2^<nd> Cucurbitaceae Genome Joint Conference
  • Place of Presentation: Kobe Convention Center,(神戸市)
  • Year and Date: 2011-12-01
• [Presentation] ダイズにおけるイオンビーム照射による葉緑素欠乏変異の生成と自殖後代における新たな変異の顕在化 (2011)
  • Author(s): 河西めぐみ
  • Organizer: 第6回高崎量子応用研究シンポジウム
  • Place of Presentation: 高崎シティギャラリー(高崎市)
  • Year and Date: 2011-10-13
• [Presentation] 外来遺伝子を持たずに特定の形質が変化した植物体の作出に必要なエピジェネティックな変化の促進 (2011)
  • Author(s): 金澤章・稲場純一・河西めぐみ・志村華子・増田税
  • Organizer: 日本育種学会第120回講演会
  • Place of Presentation: 福井市福井県立大学
  • Year and Date: 2011-09-24
• [Presentation] コサプレッションを誘導する遺伝子と共に導入した薬剤耐性遺伝子のRNAサイレンシング誘導機構 (2011)
  • Author(s): 河西めぐみ・種田晃人・金澤章
  • Organizer: 日本育種学会第120回講演会
  • Place of Presentation: 福井市福井県立大学
  • Year and Date: 2011-09-24
• [Presentation] 外来遺伝子を持たずに特定の形質が変化した植物体の作出に必要なエピジェネティックな変化の促進 (2011)
  • Author(s): 金澤章
  • Organizer: 日本育種学会第120回講演会
  • Place of Presentation: 福井県立大学(福井市)
  • Year and Date: 2011-09-24
• [Presentation] コサプレッションを誘導する遺伝子と共に導入した薬剤耐性遺伝子のRNAサイレンシング誘導機構 (2011)
  • Author(s): 河西めぐみ
  • Organizer: 日本育種学会第120回講演会
  • Place of Presentation: 福井県立大学(福井市)
  • Year and Date: 2011-09-24
• [Presentation] 花の模様形成に現れるRNAサイレンシングの誘導過程 (2011)
  • Author(s): 金澤章・河西めぐみ
  • Organizer: 日本遺伝学会第83回大会・ワークショップ「植物の機能性RNA:花成から受精まで」
  • Place of Presentation: 京都府京都大学
  • Year and Date: 2011-09-22
• [Presentation] 花の模様形成に現れるRNAサイレンシングの誘導過程 (2011)
  • Author(s): 金澤章
  • Organizer: 日本遺伝学会第83回大会・ワークショップ
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2011-09-22
• [Presentation] 低分子RNAの解析より示された隣接した2つのトランスジーンのRNAサイレンシングの誘導機構 (2011)
  • Author(s): 河西めぐみ・種田晃人・金澤章
  • Organizer: 日本遺伝学会第83回大会
  • Place of Presentation: 京都府京都大学
  • Year and Date: 2011-09-20
• [Presentation] 低分子RNAの解析より示された隣接した2つのトランスジーンのRNAサイレンシングの誘導機構 (2011)
  • Author(s): 河西めぐみ
  • Organizer: 日本遺伝学会第83回大会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2011-09-20
• [Presentation] Virus-induced gene silencing as a tool to engineer novel traits in plants : development of a TGS-inducing viral vector (2011)
  • Author(s): Akira Kanazawa
  • Organizer: Mini-International Symposium : Recent Progress of Plant Virus Research
  • Place of Presentation: Asari Classe Hotel,(小樽市)
  • Year and Date: 2011-09-18
• [Presentation] RNAサイレンシングによる遺伝子機能解析と形質改変 (2011)
  • Author(s): 金澤章
  • Organizer: 第29回日本植物細胞分子生物学会大会・シンポジウム「植物の逆遺伝学的解析技術はどこまで来たか」
  • Place of Presentation: 福岡市九州大学
  • Year and Date: 2011-09-07
• [Presentation] RNAサイレンシングによる遺伝子機能解析と形質改変 (2011)
  • Author(s): 金澤章
  • Organizer: 第29回日本植物細胞分子生物学会大会・シンポジウム
  • Place of Presentation: 九州大学(福岡市)(招待講演)
  • Year and Date: 2011-09-07
• [Presentation] ダイズ由来カロテノイド酸化開裂酵素遺伝子の機能解析 (2011)
  • Author(s): 山田哲也
  • Organizer: 第29回日本植物細胞分子生物学会大会
  • Place of Presentation: 九州大学(福岡市)
  • Year and Date: 2011-09-07
• [Presentation] エピジェネティックな変化を誘導することで遺伝子組換えによらずに形質を変化させた植物体の作出 (2011)
  • Author(s): 金澤章
  • Organizer: 日本育種学会第119回講演会
  • Place of Presentation: 横浜市立大学(横浜市)
  • Year and Date: 2011-04-14
• [Presentation] 隣接したトランスジーン間の相互作用によるRNAサイレンシングの誘導機構 (2011)
  • Author(s): 河西めぐみ
  • Organizer: 日本育種学会第119回講演会
  • Place of Presentation: 横浜市立大学(横浜市)
  • Year and Date: 2011-04-14
• [Presentation] The inheritable transcriptional gene silencing targeted to an endogenous gene in petunia by using the Cucumber mosaic virus vector (2011)
  • Author(s): Inaba, J., Kanazawa, A., Shimura, H., Otagaki, S., Tsukahara, S., Matsuzawa, A., Kim, B., Goto, K. and Masuta, C
  • Organizer: Keystone Symposia on Molecular and Cellular Biology
  • Place of Presentation: Portola Hotel & Spa, Monterey, California, USA
  • Year and Date: 2011-03-22
• [Presentation] The inheritable transcriptional gene silencing targeted to an endogenous gene in petunia by using the Cucumber mosaic virus vector (2011)
  • Author(s): Jun-ichi Inaba
  • Organizer: Keystone Symposia on Molecular and Cellular Biology
  • Place of Presentation: Portola Hotel & Spa, Monterey, California
  • Year and Date: 2011-03-11
• [Presentation] イオンビーム照射によるダイズ突然変異体集団の効率的作出に向けた可視的変異の解析 (2010)
  • Author(s): 金澤章
  • Organizer: 第5回高崎量子応用研究シンポジウム
  • Place of Presentation: 高崎市高崎シティギャラリー
  • Year and Date: 2010-10-15
• [Presentation] Read-through転写が関わる隣接したトランスジーン間の相互作用によるRNAサイレンシングの誘導 (2010)
  • Author(s): 河西めぐみ・種田晃人・金澤章
  • Organizer: 日本遺伝学会第82回大会
  • Place of Presentation: 札幌市北海道大学
  • Year and Date: 2010-09-20
• [Presentation] 花の模様形成におけるRNAサイレンシング (2010)
  • Author(s): 金澤章
  • Organizer: RNAから植物を考える～植物RNA研究の最先端～
  • Place of Presentation: 札幌市北海道大学(招待講演)
  • Year and Date: 2010-08-26
• [Presentation] RNA-mediated epigenetic modifications by a viral vector: a potent system to produce a plant that does not carry a transgene but has altered traits.
  • Author(s): Akira Kanazawa
  • Organizer: 10th International Congress on Plant Molecular Biology
  • Place of Presentation: ICC, Jeju, Republic of Korea
• [Presentation] Coincident RNA silencing of linked transgenes in the plant genome mediated by read-through transcription.
  • Author(s): Megumi Kasai
  • Organizer: 10th International Congress on Plant Molecular Biology
  • Place of Presentation: ICC, Jeju, Republic of Korea
• [Presentation] Transcription and transposition of the soybean retrotransposon SORE-1.
  • Author(s): Mayumi Tsuchiya
  • Organizer: 10th International Congress on Plant Molecular Biology
  • Place of Presentation: ICC, Jeju, Republic of Korea
• [Presentation] エピジェネティックな遺伝子発現制御を介した形質改変
  • Author(s): 金澤 章
  • Organizer: 日本育種学会第54回シンポジウム
  • Place of Presentation: 京都市 京都産業大学
• [Presentation] エピジェネティックな機構による転写抑制の解除を利用したin planta アッセイ系により明らかになった植物成分のDNAメチル化抑制効果
  • Author(s): 金澤 章
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 福岡市 九州大学
• [Presentation] ダイズのレトロトランスポゾンSORE-1の発現パターン解析
  • Author(s): 土屋真弓
  • Organizer: 日本育種学会第122回講演会
  • Place of Presentation: 京都市 京都産業大
• [Presentation] ペチュニア形質転換体における長期間の育成により誘導されたコサプレッションからの復帰
  • Author(s): 河西めぐみ
  • Organizer: 日本育種学会第122回講演会
  • Place of Presentation: 京都市 京都産業大
• [Presentation] イオンビーム照射によって得られた葉緑素欠乏ダイズ系統における種子成分の変化
  • Author(s): 土屋真弓
  • Organizer: 第7回高崎量子応用研究シンポジウム
  • Place of Presentation: 高崎市 高崎シティギャラリー
• [Presentation] ディープシークエンス解析によって明らかになった、ペチュニアにおけるコサプレッションとnaturally occurring RNAサイレンシングの低分子RNA産生機構の共通性
  • Author(s): 河西めぐみ
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 福岡市 九州大学
• [Book] Gene duplication and RNA silencing in soybean. In A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships (2012)
  • Author(s): Kasai, M., Tsuchiya, M. and Kanazawa, A
  • Publisher: Ed. by J. E. Board, InTech
• [Book] A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships (2012)
  • Author(s): Megumi Kasai
  • Total Pages: 613
  • Publisher: InTech
• [Book] 植物の分子育種学 (2011)
  • Author(s): 金澤章
  • Publisher: 講談社
• [Book] 植物の分子育種学 (2011)
  • Author(s): 鈴木正彦・編著
  • Total Pages: 215
  • Publisher: 講談社