Exploitation of recombination-enhancing factors for enhancement of breeding efficiency

• Project/Area Number: 22658003
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Breeding science
• Research Institution: Tottori University
• Principal Investigator: TSUJIMOTO Hisashi 鳥取大学, 乾燥地研究センター, 教授 (50183075)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,700,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥600,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,100,000 (Direct Cost: ¥1,100,000)
• Keywords: 減数分裂 / 組換え / 染色体 / コムギ / 異種染色体添加系統

Research Abstract

Breeding efficiency will be increased if the factors promoting meiotic recombination will be discovered. However, because homologous chromosomes cannot be discriminated usually, there are no experimental systems to detect it. In this study, we produced wheat lines carrying two alien chromosomes each of which are derived from different species, and found lines in which two alien chromosomesassociated in the meiotic prophase. In addition, we found that zebularine induced chromosome rearrangements in mitosis and affected slightly the meiotic chromosome pairing. In addition, we tried to introduce ph1b gene to the alien chromosome addition lines.

Research Products

• [Journal Article] Homology of two alien chromosomes during meiosis in wheat (2011)
  • Author(s): Cho, S.-W., Moritama, Y., Ishii, T., Kishii, M., Tanaka, H., Eltayeb,A.E., Tsujimoto, H
  • Journal Title: Chromosome Science Volume: 14 Pages: 45-52
  • URL: https://www.jstage.jst.go.jp/browse/scr/14/0/_contents
  • Peer Reviewed
• [Journal Article] Effects of the cytidine analogue zebularine on wheat mitotic chromosomes. (2011)
  • Author(s): Cho, S.-W., Ishii, T., Matsumoto, N., Tanaka, H., Eltayeb, A.E., Tsujimoto,H.
  • Journal Title: Chromosome Science Volume: 14 Pages: 23-28
  • URL: https://www.jstage.jst.go.jp/browse/scr/14/1+2/_contents
  • Peer Reviewed
• [Journal Article] Effects of the cytidine analogue zebularine on wheat mitotic chromosomes (2011)
  • Author(s): Cho S-W, Ishii T, Matsumoto N, Tanaka H, Eltaveb AE, Tsuiimoto H
  • Journal Title: Chromosome Science Volume: 14 Pages: 23-28
  • Peer Reviewed
• [Journal Article] Homology of two alien chromosomes during meiosis in wheat (2011)
  • Author(s): Cho S-W, Moritama Y, Ishii T, Kishii M, Tanaka H, Eltaveb AE
  • Journal Title: Chromosome Science Volume: 14(印刷中)
  • Peer Reviewed
• [Presentation] Production of alien chromosome addition wheat lines to identify relatedness between alien chromosomes (2012)
  • Author(s): Cho, S.-W., Moritama, Y., Ishii, T., Kishii, M., Tanaka, H., Eltayeb, A. E., and Tsujimoto, H
  • Organizer: 日本育種学会第121回講演会
  • Place of Presentation: 栃木県宇都宮市
  • Year and Date: 2012-03-29
• [Presentation] Production of alien chromosome addition wheat lines to identify relatedness between alien chromosomes (2012)
  • Author(s): Cho SW, Moritama Y, Ishii T, Kishii M, Tanaka H, Eltaveb AE
  • Organizer: 日本育種学会
  • Place of Presentation: 宇都宮大学(宇都宮市)
  • Year and Date: 2012-03-29
• [Presentation] Molecular cytogenetical studies on meiotic chromosome association in wheat lines with chromosomes of Leymus species (2010)
  • Author(s): Cho S-W, Moritama Y, Ishii T, Tanaka H, Tsujimoto H
  • Organizer: 7^<th> International Joint Symposium between Korea and Japan
  • Place of Presentation: Chunchean, Korea
  • Year and Date: 2010-11-11
• [Presentation] Effect of zebularine on wheat mitotic chromosomes (2010)
  • Author(s): Cho S-W, Ishii T, Tanaka H, Tsujimoto H
  • Organizer: 染色体学会第61回年会
  • Place of Presentation: 習志野、千葉
• [Remarks] 雑誌論文の(1)が染色体学会論文賞を受賞した。