New mutagenesis approaches to study gene function in crop
| Project/Area Number |
22658004
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Breeding science
|
|---|
| Research Institution | University of Shizuoka |
|---|
Principal Investigator |
IIDA Shigeru 静岡県立大学, 大学院・食品栄養環境科学研究院, 客員教授 (30012777)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KOBAYASHI Hirokazu 静岡県立大学, 大学院・食品栄養環境科学研究院, 教授 (80170348)
NOGUCHI Hiroshi 静岡県立大学, 薬学部, 教授 (60126141)
JOHZUKA Yasuyo (HISATOMI Yasuyo) 静岡県立大学, 大学院・食品栄養環境科学研究院, 客員研究員 (60517887)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,580,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
|
|---|
| Keywords | 植物 / 突然変異 / 育種学 / 逆遺伝学 / バイオテクノロジー / 遺伝子 / ゲノム / 遺伝学 / 遺伝的組換え |
|---|
| Research Abstract |
To characterize gene function effectively, we tried to develop reverse genetic approaches, namely conditional knock-out and generation of subtle mutations by homologous recombination-mediated gene targeting and gene tagging employing endogenous nDart1-related DNA transposons. We also characterized insertion mutants isolated by gene targeting and tagging.
|
Report
(4 results)
Research Products
(19 results)
