| Project/Area Number |
22658005
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Crop science/Weed science
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| Research Institution | Nagoya University |
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Principal Investigator |
MIYAKE Hiroshi 名古屋大学, 生命農学研究科, 教授 (60134798)
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| Co-Investigator(Kenkyū-buntansha) |
YAMANE Koji 近畿大学, 農学部, 講師 (50580859)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,160,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | 塩ストレス / 過酸化水素 / 活性酸素 / 作物 / 組織化学 / 電子顕微鏡 / 植物 / ストレス / 作物学 |
|---|
| Research Abstract |
Histochemical methods were developed to observe distribution of hydrogen peroxide in the cells of crop plants. In the CeCl3 method, leaf segments were incubated in 5 mM CeCl3 at pH 7. 2 for 1 h, fixed, dehydrated and embedded for electron microscopy. In the DAB method, leaf segments were fixed in Karnovski's fixative for 2 h and incubated in 10 mM DAB at pH 8. 0 for 1 h. After incubation the segments were prepared for electron microscopy. Hydrogen peroxide was detected around the grana of chloroplasts in salinity-treated rice plants.
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