Analysis of the dominant double-flower gene using high density RAD markers in Eustoma grandiflorum

• Project/Area Number: 22658007
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Horticulture/Landscape architecture
• Research Institution: The University of Tokyo
• Principal Investigator: KAWABATA Saneyuki 東京大学, 大学院・農学生命科学研究科, 准教授 (10234113)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,530,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: 花卉 / トルコギキョウ / DNAマーカー / 八重咲き / 次世代シークエンサー / ABCモデル

Research Abstract

Linkage mapping of dominant double-flower trait was conducted in Eustoma grandiflorum. In the F2 population constructed between double-flower and single-flower accessions, double-flower with more than two whorls and flowers with abnormal floral organs were observed. The segregation ratio suggested that more than two genes were involved in the formation of double flowers. A total of 800k RAD tags was determined and 100k SNPs were found in these RAD tags. Linkage analysis was performed using these RAD markers and F2 population.

Research Products

• [Journal Article] EST sequencing and microarray analysis of the floral transcriptome of Eustoma grandiflorum (2012)
  • Author(s): Kawabata S, Li Y., Miyamoto K.
  • Journal Title: Scientia Horticulturae Volume: (in print)
• [Journal Article] Three-dimensional formation of corolla shapes in relation to the developmental distortion of petals in Eustoma grandiflorum (2011)
  • Author(s): Kawabata S, Yokoo M., Nii K.
  • Journal Title: Scientia Horticulturae Volume: 132 Pages: 66-70
• [Journal Article] Double fertilization and embryogenesis of Eustoma grandiflorum (2011)
  • Author(s): Wang Q, Zhang Y, Kawabata S, and Li Y.
  • Journal Title: J.Japan.Soc.Hort.Sci. Volume: 80 Pages: 351-357
  • NAID: 40018904639
• [Presentation] トルコギキョウの花器官におけるMADS-box遺伝子の発現 (2012)
  • Author(s): 石森 元幸・宮坂昌実・河鰭実之
  • Organizer: 園芸学会平成24年度秋季大会
• [Presentation] トルコギキョウの花弁成長に伴う遺伝子発現プロファイルのマイクロアレイ解析 (2011)
  • Author(s): 河鰭実之・宮本健太郎
  • Organizer: 園芸学会平成23年度春季大会
  • Place of Presentation: 宇都宮大学
• [Presentation] 八重咲きトルコギキョウにおける花器官の形成と花器官形成関連遺伝子の発現 (2011)
  • Author(s): 徐品三, ほか4名
  • Organizer: 園芸学会平成22年度秋季大会
  • Place of Presentation: 大分県
• [Presentation] 次世代シークエンサーを利用したトルコギキョウの花におけるcDNAデータベースの構築 (2010)
  • Author(s): 宮本健太郎・新居加恵子・河鰭実之
  • Organizer: 園芸学会平成22年度秋季大会
  • Place of Presentation: 大分大学(大分県)
  • Year and Date: 2010-09-19
• [Presentation] 八重咲きトルコギキョウにおける花器官の形成と花器官形成関連遺伝子の発現 (2010)
  • Author(s): 徐品三, ほか4名
  • Organizer: 園芸学会平成22年度秋季大会
  • Place of Presentation: 大分大学(大分県)
  • Year and Date: 2010-09-19