Structure and function of cadmium adsorbing materials in higher plants.

Research Project

Project/Area Number	22658021
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Plant nutrition/Soil science
Research Institution	The University of Tokyo
Principal Investigator	YOSHIMURA Etsuro 東京大学, 大学院・農学生命科学研究科, 教授 (10130303)
Project Period (FY)	2010 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥3,560,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥360,000) Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2010: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywords	植物栄養代謝 / カドミウム / タチスベリヒユ / NMR / 糖鎖 / bathocuproinesulfonat / コマツナ / カドミウム結合物質 / TPPS / チオール基
Research Abstract	Majority of cadmium was distributed in precipitated fraction of homogenate of the higher plant Portulaca oleracea. Digestion of the precipitate with pectinase released cadmium in the solution. Cadmium-binding substance was purified from the solution using several steps of chromatography, where detection method for soft-metal chelator that was newly developed was applied. The structure of the purified substance was determined to be polysaccharides by NMR spectrometry.