Genetic modification of Agrobacterium tumefaciens in order to use chloroplast transformation.

Research Project

Project/Area Number	22658025
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Applied microbiology
Research Institution	Shinshu University
Principal Investigator	NOGAWA Masahiro 信州大学, 繊維学部, 准教授 (10283037)
Project Period (FY)	2010 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥3,470,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥270,000) Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2010: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywords	微生物遺伝・育種 / DNA伝達 / Agrobacterium tumefaciens / 形質転換 / 葉緑体
Research Abstract	Chloroplast transformation to incorporate a transgene into the chloroplast DNA and the transgene expression in chloroplast is suitable for high production of useful proteins in plants. Biolistic transformation with particle gun was used for chloroplast transformation. In this study, VIRD2 and VIRE2 proteins of Agrobacterium tumefaciens which transport bacterial T-DNA into plant nucleus were modified to convey the T-DNA into chloroplast. A. tumefaciens containing modified VIRD2 and VIRE2 would transform chloroplast of plants.