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Molecular identification of nitrogen-transporter and analysis of nitrogen-fixing reaction in bacteria

Research Project

Project/Area Number 22658026
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionKyoto University

Principal Investigator

MURATA Kousaku  京都大学, 農学研究科, 教授 (90142299)

Co-Investigator(Kenkyū-buntansha) 河井 重幸  京都大学, 農学研究科, 助教 (00303909)
Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,490,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywords窒素輸送体 / 窒素固定 / 細胞表層構造 / ポーリン様構造体 / ガス生物学 / Azotovacter vinelandii / アルギン酸 / トランスアルジソーム / 小胞状構造体 / Azotobacter vinelandii / 膜タンパク質 / 酸素耐性機構
Research Abstract

Although Azotobacter vinelandii can grow by using atomospheric nitrogen(N_2) gas as a sole nitrogen source, the knowledge on the nitrogen transport across the cell membrane has not been accumulated. In this study, the existence of nitrogen gas chanell was examined by growing the bacterial cells in the presence of extremely low nitrogen gas concentrations, and found that some genes are strongly induced under the conditions.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (6 results)

All 2011 2010

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (4 results)

  • [Journal Article] Azotobacter vinelandii gene clusters for two types of peptidic and catechol siderophores produced in response to molybdenum2011

    • Author(s)
      Fuminori Yoneyama, Mayumi Yamamoto, Wataru Hashimoto and Kousaku Murata
    • Journal Title

      J. Appl. Microbiol

      Volume: 111 Issue: 4 Pages: 932-938

    • DOI

      10.1111/j.1365-2672.2011.05109.x

    • Related Report
      2011 Final Research Report
  • [Journal Article] Azotobacter vinelandii gene clusters for two types of peptidic and catechol siderophores produced in response tb molybdenum2011

    • Author(s)
      Fuminori Yoneyama
    • Journal Title

      Journal of Applied Microbiology

      Volume: 111 Pages: 932-938

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 窒素固定酢酸菌Gluconacetobacter diazotorophicusのリン酸依存的生育挙動2011

    • Author(s)
      天本隆太
    • Organizer
      第3回リン化合物討論会(第30回C-P化合物研究会)
    • Place of Presentation
      岡山大学岡山市
    • Year and Date
      2011-11-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] エンドファイティック窒素固定酢酸菌Gluconacetobacter diazotorophicusのリン酸依存的生育挙動2011

    • Author(s)
      天本隆太
    • Organizer
      日本生物工学会平成23年度大会
    • Place of Presentation
      東京農工大学小金井市
    • Year and Date
      2011-09-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] エンドファイティック窒素固定酢酸菌Gluconobacter diazotorophicusの窒素非含有固体培地における生育挙動2011

    • Author(s)
      天本隆太, 村田幸作, 他
    • Organizer
      日本農芸化学会 2011年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 窒素固定細菌Azotobacter vinelandiiのシデロフォア生合成遺伝子クラスター2010

    • Author(s)
      米山史紀、山本真由美、橋本渉、村田幸作
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-29
    • Related Report
      2011 Final Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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