| Project/Area Number |
22658029
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Okayama University of Science |
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Principal Investigator |
HARA Hirofumi 岡山理科大学, 工学部, 准教授 (80511071)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,740,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | 放線菌 / ロドコッカス属 / ゲノム / 抗生物質生産 / 転写誘導 |
|---|
| Research Abstract |
Rhodococcus jostii RHA1 was grown on a broad range of aromatic compounds and vigorously degrades environmental pollutants, such as polychlorinated biphenyls (PCBs). Based on the genome analysis of RHA1, many secondary metabolites genes including polyketide synthase (PKS) and non-ribosomal dependent peptide synthase (NRPS) were found on chromosome and plasmids, as same as other actinomycetes. In this study, induction conditions of these genes were investigated to understand what isa role of secondary metabolites from these genes on particular conditions. Type I, type III, PKS-NRPS hybrid, and NRPSs on plasmid were mainly induced on solid medium. On the other hands, type II PKSs were induced on liquid medium. For understanding of the secondary metabolism in actinomycetes, we are now trying to analyze chemical composition of these secondary metabolites.
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