| Budget Amount *help |
¥3,670,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Research Abstract |
We have newly developed two methods for RNA aptamer selection: one is a kinetically designed RNA aptamer selection method. The second is a rapid fluorescent labeling method for RNA. Using these methods, we have selected RNA aptamers to bacterial spores from random RNApopulation. The RNA aptamers were isolated from selected RNA population by cloning and characterized. All RNA aptamers bind to bacterial spores specifically, but do not bind to vegetative bacterial cellsat all. The aptamers bind to bacterial spores from any species tested. It has been concluded that the RNA aptamers recognize some structures commonly present in bacterial spores.
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