| Project/Area Number |
22658037
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SAKUDA Shohei 東京大学, 大学院・農学生命科学研究科, 准教授 (80192087)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥600,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | 生物活性物質 / 標的分子 / プローブ / 標的タンパク質 / アロサミジン / プレコセン / 2量体化 / 生理活性 / 生物活性 / ダイマー |
|---|
| Research Abstract |
Identification of the target molecule of a bioactive compound is most important for clarifying the mode of action of the compound. The target molecule can afford a clue to investigate the unknown biological phenomenon concerning the bioactive compound at the molecular level. In this study, we attempted to develop a novel method for identifying the target molecule using a dimer probe. Mycotoxin production inhibitors and allosamidin, a chitinase inhibitor, were used as model bioactive compounds. A dimer of precocene II, specific inhibitor of trichothecenes, was prepared based on the structure-activity relationship work, but no dimer with inhibitory activity was obtained. Therefore, we tried to investigate its target molecule using click chemistry and magnetic affinity-beads methods, which are recently developed, by comparing the methods with conventional photoaffinity probe method, and we have gotten a candidate protein as the target molecule. Preparation of allosamidin dimers is ill in progress because it is difficult to connect two allosamidin molecules at desirable positions.
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