A new feed profiling using DNA information for wild-herbivores

• Project/Area Number: 22658050
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Forest science
• Research Institution: Kyushu University
• Principal Investigator: SHIRAISHI Susumu 九州大学, 大学院・農学研究院, 教授 (70226314)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,580,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: 草食性動物 / シカ / 食性 / DNA塩基長多型 / 葉緑体DNA / DNAバーコーディング

Research Abstract

To facilitate the studies on wild-herbivore feeding, a simple feed profiling based on feces' DNA analysis was developed using several sequence length polymorphisms recognized in chloroplast intergenic spacer regions(trnD. trnY, trnF-trnL, trnP-trnW, etc.) instead of DNA barcoding. A database on the sequence length polymorphism in these spacer regions was constructed for identifying the fed plant species quickly.

Research Products

• [Presentation] 採食履歴のあるシカ糞のDNA Feed Profile分析 (2011)
  • Author(s): 盛島司, 堀野眞一, 野宮治人, 白石進
  • Organizer: 第122回日本森林学会大会
  • Place of Presentation: 静岡大学(静岡)
  • Year and Date: 2011-03-29
• [Presentation] 採食履歴のあるシカ糞のDNA Feed Profile分析 (2011)
  • Author(s): 盛島司, 堀野眞一, 野宮治人, 白石進
  • Organizer: 日本森林学会大会
  • Place of Presentation: 静岡(静岡大学)
  • Year and Date: 2011-03-27
• [Presentation] 採食履歴のあるシカ糞のDNA Feed Profile分析 (2011)
  • Author(s): 盛島司,堀野眞一,野宮治人,白石進
  • Organizer: 第122回日本森林学会大会
  • Place of Presentation: 静岡(静岡大学)
• [Presentation] 糞サンプルを用いた草食性動物の簡便な食性プロファイリング (2010)
  • Author(s): 盛島司, 川邊縫, 川副まり子, 白石進, 池田浩一
  • Organizer: 日本森林学会大会
  • Place of Presentation: つくば(筑波大学)
  • Year and Date: 2010-04-04
• [Presentation] 糞サンプルを用いた草食性動物の簡便な食性プロファイリング (2010)
  • Author(s): 盛島司,縫,川副まり子,白石進,池田浩一
  • Organizer: 第121回日本森林学会大会
  • Place of Presentation: つくば(筑波大学)