| Project/Area Number |
22658053
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Wood science
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IMAI Tomoya 京都大学, 生存圏研究所, 准教授 (90509142)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,070,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | セルロース / セルロース合成酵素 / CesA / 酵素反応速度論 / c-di-GMP / 酢酸菌 / CesABCD / 大腸菌内再構成 / c-diGMP / 協同性 |
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| Research Abstract |
The mechanism how cellulose synthase produces cellulose is not understood well, because this enzyme is very difficult to investigate and the enzymatic activity has been rarely analyzed in direct. In order to conduct direct analysis of cellulose synthase, three materials are prepared in this project :(i) recombinant proteins of cellulose synthase complex by E. coli expression system,(ii) c-di-GMP synthesis in vitro and in E. coli to fully activate the enzyme and(iii) enzymatic kinetics of cellulose-synthesizing activity in vitro. All of these are very important for direct analysis of cellulose synthase ; especially(i) is the first example in the world and an outstanding research resource. Thus this study has set up important tools for studying cellulose biosynthesis deeply.
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