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Development of growth inhibition of fish pathogenic virus by RNA aptamers

Research Project

Project/Area Number 22658059
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field General fisheries
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

AOKI Takashi  東京海洋大学, 海洋科学技術研究科, 特任教授 (00051805)

Co-Investigator(Kenkyū-buntansha) 廣野 育生  東京海洋大学 (00270926)
近藤 秀裕  東京海洋大学 (20314635)
Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,650,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsRNAアプタマー / 魚類病原ウイルス / ウイルス性出血性敗血症 / ヒラメラブドウイルス病 / SELEX法 / Rhodovulum sulfidophilum / MarineBiotechnology / ヒラメ / Marine Biotechnology
Research Abstract

The selected RNA aptamers against viral hemorrhagic septicemia virus(VHSV) and hirame rhabdovirus(HIRRV) showed highest specificity and affinity for the respective fish pathogenic viruses
Hirame natural embryo(HINAE) cells treated with the viruses and the corresponding RNA aptamers showed a decrease in incidence of cytopathic effect(CPE) compared to controls(treated only with virus)
Rhodovulum sulfidophilum transformed with genes encoding for VHSV-and HIRRV-aptamers produced their extracellular aptamers on a large scale. When fish was cultured in seawater containing R. sulfidophilum which produces VHSV-RNA aptamers, fish mortality during VHSV challenge decreased. It reveals that the recombinant R. sulfidophilum can be a powerful tool for prevention of virus infection in fish

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (9 results)

All 2012 2011 2010 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (7 results)

  • [Journal Article] RNA aptamer(s) inhibit the growth of the fish pathogen viral hemorrhagic septicemia virus (VSHV)2012

    • Author(s)
      Punnarak, P., Santos, M. D., Hwang, S. D., Kondo, H., Hirono, I., Kikuchi, Y., and Aoki, T.
    • Journal Title

      Marine Biotechnology

      Volume: 14 Issue: 6 Pages: 752-761

    • DOI

      10.1007/s10126-012-9448-1

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of hirame rhabdovirus(HIRRV) growth by RNA aptamers

    • Author(s)
      Seong Don Hwang, Naoko Midorikawa, Porntep Punnarak, Yo Kikuchi, Hidehiro Kondo, Ikuo Hirono, Takashi Aoki
    • Journal Title

      Journal of Fish Diseases

      Volume: (投稿中)

    • Related Report
      2011 Final Research Report
  • [Presentation] Inhibition of hirame rhabdovirus(HIRRV) growth by newly developed RNA aptamers2012

    • Author(s)
      Seong Don Hwang, Porntep Punnarak, Hidehiro Kondo, Ikuo Hirono, Takashi Aoki
    • Organizer
      平成24年度日本魚病学会春季大会
    • Place of Presentation
      東京海洋大学、東京
    • Related Report
      2011 Final Research Report
  • [Presentation] Inhibition of hirame rhabdovirus (HIRRV) growth by newly developed RNA aptamers2012

    • Author(s)
      Seong Don Hwang, Porntep Punnarak, Hidehiro Kondo, Ikuo Hirono, Takashi Aoki
    • Organizer
      平成24年度日本魚病学会春季大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Antiviral ability of RNA apatamers against Hirame rhabdovirus2011

    • Author(s)
      Porntep Punnarrak, Hidehiro Kondo, IkuoHirono, Takashi Aoki
    • Organizer
      平成23年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(品川キャンパス)
    • Related Report
      2011 Final Research Report
  • [Presentation] Virulence inhibition of fish virus by RNA aptamers2011

    • Author(s)
      Aoki Takashi, Seong Dong Hwang, Porntep Punnarak, Hidehiro Kondo, Ikuo Hirono
    • Organizer
      15^<th> EAFP International Conference on Diseases of Fish and Shellfish
    • Place of Presentation
      Split, Croatia
    • Related Report
      2011 Final Research Report
  • [Presentation] Study on protection of RNA aptamers against viral hemorrhagic septicemia virus infection in Japanese flounder2011

    • Author(s)
      Porntep Punnarak, Mudjekeewis D. Santos, Seong Don Hwang, Hidehiro Kondo, IkuoHirono, Takashi Aoki
    • Organizer
      平成23年度日本水産学会秋季大会
    • Place of Presentation
      長崎大学文京キャンパス
    • Related Report
      2011 Final Research Report
  • [Presentation] Growth inhibition of viral hemorrhagic septicemia virus by RNAaptamers.2010

    • Author(s)
      Pomtep Punnarak・Mudjekeewis D.Santos・Hiromichi Suzuki・Yo Kikuchi・Hidehiro Kondo・Ikuo Hirono・Takashi Aoki.
    • Organizer
      2010度日本水産学会秋季大会
    • Place of Presentation
      京都大学吉田キャンパス(京都府)
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Growth inhibition of viral hemorrhagic septicemia virus by RNA aptamers2010

    • Author(s)
      Porntep Punnarak, Mudjekeewis D. Santos, Hiromichi Suzuki, Yo Kikuchi, HidehiroKondo, Ikuo Hirono, Takashi Aoki
    • Organizer
      平成22年度日本水産学会大会
    • Place of Presentation
      京都大学吉田キャンパス
    • Related Report
      2011 Final Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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