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Establishment of the artificial digestion method using a gastrointestinal tract of fish

Research Project

Project/Area Number 22658061
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field General fisheries
Research InstitutionTokai University

Principal Investigator

KIHARA Minoru  東海大学, 生物理工学部・海洋生物科学科, 教授 (40405684)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,520,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥120,000)
Fiscal Year 2011: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2010: ¥3,000,000 (Direct Cost: ¥3,000,000)
Keywords魚類 / 培養胃 / ペプシン / 人工消化 / 飼料 / タンパク質 / 増養殖 / 胃 / in vitro / 培養 / 養魚飼料
Research Abstract

Gastric digestion was studied in the isolated ex vivo stomach of rockfish. The digestibility of the ex vivo stomach was compared to in vivo and in vitro stomachs so that this technique could be used as a new artificial gastric digestion method. The digestibility was varied among groups(in vivo ; 68%, ex vivo ; 51%, in vitro ; 33%, respectively), however, the ex vivo technique appears a better digestibility than the artificial digestion using an in vitro test. These findings suggest that the ex vivo stomach should be useful in evaluation of fish gastric digestion, as a new artificial method.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (18 results)

All 2011 2010 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (9 results) Book (1 results) Remarks (5 results)

  • [Journal Article] Stimulative effect of skipjack tuna soluble extract on pepsin-like protease in the stomach of rockfish(Sebastes schlegelii) using an in vitro perfusion method2011

    • Author(s)
      Minoru Kihara, Makoto Igarashi, Tomohiro Suzuki, Fumiaki Itou, Satoshi Kozeni, Mihoko Toyomane, Junko Nakano, Ikumi Yamai
    • Journal Title

      Comparative Biochemistry and Physiology

      Volume: 158A Pages: 444-449

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stimulative effect of skipjack tuna soluble extract on pepsin-like protease in the stomach of rockfish (Sebastes schlegelii) using an in vitro perfusion method.2011

    • Author(s)
      Minoru Kihara
    • Journal Title

      Comparative Biochemistry and Physiology

      Volume: 158A Pages: 444-449

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Acidity and pepsin-like protease activity of the gastric juice of farmed Pacific bluefin tuna Thunnus orientalis

    • Author(s)
      Minoru Kihara, Yutaro Abe, Hiroshi Kaga
    • Journal Title

      Aquaculture Science

      Volume: (in press)

    • NAID

      10031122360

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Presentation] クロソイ生体胃内および培養胃内での消化2011

    • Author(s)
      木原稔
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      長崎大学(長崎)
    • Year and Date
      2011-09-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] 胃刺激物質添加飼料のクロソイ培養胃内での消化亢進2011

    • Author(s)
      佐藤結衣・鈴木智博・木原稔
    • Organizer
      平成23年度日本水産学会春季大会
    • Related Report
      2011 Final Research Report
  • [Presentation] クロソイ生体胃内および培養胃内での消化2011

    • Author(s)
      佐藤結衣・鈴木智博・木原稔
    • Organizer
      平成23年度日本水産学会秋季大会
    • Related Report
      2011 Final Research Report
  • [Presentation] 魚類飼料開発用臓器ツールとしての魚類摘出培養胃の有用性2010

    • Author(s)
      木原稔
    • Organizer
      Hindgut Club Japan Symposium
    • Place of Presentation
      専修大学神田校舎(東京都)
    • Year and Date
      2010-12-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] 培養胃内消化の誘起には飼料溶出成分が関与する2010

    • Author(s)
      木原稔
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      京都大学(京都府)
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] カツオ煮汁濃縮液はクロソイ胃のペプシン様酵素分泌を刺激する2010

    • Author(s)
      佐藤結衣・木原稔
    • Organizer
      平成22年度日本農芸化学会北海道支部大会
    • Related Report
      2011 Final Research Report
  • [Presentation] 培養胃内消化の誘起には飼料溶出成分が関与する2010

    • Author(s)
      佐藤結衣・木原稔
    • Organizer
      平成22年度日本水産学会秋季大会
    • Related Report
      2011 Final Research Report
  • [Presentation] 飼料中への胃刺激物質:カツオエキスの添加によるクロソイ稚魚の成長促進作用2010

    • Author(s)
      佐藤結衣・木原稔
    • Organizer
      平成22年度日本水産学会北海道支部大会
    • Related Report
      2011 Final Research Report
  • [Presentation] 魚類飼料開発用臓器ツールとしての魚類摘出培養胃の有用性2010

    • Author(s)
      木原稔
    • Organizer
      2010年度Hindgut Club Japan Symposium
    • Related Report
      2011 Final Research Report
  • [Book] 消化管の生理機能と腸内細菌2011

    • Author(s)
      木原稔
    • Publisher
      アニマルメディア社
    • Related Report
      2011 Annual Research Report
  • [Remarks] 科学新聞(2011.3.18掲載)

    • Related Report
      2011 Final Research Report
  • [Remarks] 北海道新聞朝刊(2012.1.27掲載)

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.u-tokai.ac.jp/

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.u-tokai.ac.jp/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.u-tokai.ac.jp/

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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