| Project/Area Number |
22658078
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Agricultural information engineering
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OMASA Kenji 東京大学, 大学院・農学生命科学研究科, 教授 (70109908)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,560,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | イメージング / 共焦点レーザ顕微鏡 / 植物計測 / 3D / クロロフィル蛍光 / 細胞 / 環境応答 |
|---|
| Research Abstract |
Confocal laser scanning micro-imaging(CLSM) analysis method of chloroplast photosynthetic functions in intact leaf was developed. For A/D conversion levels of EM-CCD camera used for the CLSM system the values revealed a positive linear relationship between fluorescence intensity and the A/D conversion level at each gain value in the range 500 to 14, 500. The relationship between laser power and measured PPF was expressed as a linear equation. Using this system, 3D-F, 3D-Fm' and 3D-Φ_<PSII> images of Boston fern chloroplasts were measured. The dark/light transitions(Kautsky effect) of eggplant chloroplasts to agrochemical treatment were also measured. As the 3D CLSM system can provide the fluorescence intensity,Φ_<PSII> and NPQ values of each chloroplast in a 3D cellular arrangement, this method has potential for investigating differences in the functions of chloroplasts in vivo.
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