Establishment of sperm cells in vitro from female mouse somatic cells

• Project/Area Number: 22658086
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Applied animal science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: KANEDA Masahiro 独立行政法人農業・食品産業技術総合研究機構, 畜産草地研究所・家畜育種繁殖研究領域, 主任研究員 (80469840)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: 発生工学 / 生殖細胞 / DNAメチル化 / ゲノムインプリンティング / エピジェネティクス / インプリンティング

Research Abstract

Genomic imprinting is indispensable for the proper mammalian development and is established during gametogenesis. I focused on DNA methylation, which plays an essential role for the establishment of imprinting, to create sperm-like cells from female mouse somatic cells in vitro. Three different types of DNA methylation inhibitors were added to mouse embryonic fibroblast and DNA methylation status was analyzed. There was a decrease of DNA methylation levels in some imprinted genes, however, no inhibitors could induce hypo-methylation in all imprinted genes analyzed.

Research Products

• [Journal Article] Genomic imprinting in mammals-Epigenetic parental memories. (2011)
  • Author(s): Masahiro Kaneda
  • Journal Title: Differentiation Volume: Vol.82 Pages: 51-56
  • Peer Reviewed
• [Journal Article] Comparison of DNA methylation levels of repetitive loci during bovine development (2011)
  • Author(s): Masahiro Kaneda, Satoshi Akagi, Shinya Watanabe and Takashi Nagai
  • Journal Title: BMC Proceedings Volume: Vol.5 Issue: S4
  • DOI: 10.1186/1753-6561-5-s4-s3
  • Peer Reviewed
• [Journal Article] Genomic imprinting in mammals-Epigenetic parental memories (2011)
  • Author(s): 金田正弘
  • Journal Title: Differentiation Volume: 82巻 Issue: 2 Pages: 51-56
  • DOI: 10.1016/j.diff.2011.05.004
  • Peer Reviewed
• [Presentation] 体細胞クローン雌牛の卵子DNAメチル化解析 (2011)
  • Author(s): 金田正弘、渡辺伸也、赤木悟史、原口清輝、Somfai Tamas、稲葉泰志、下司雅也、永井卓
  • Organizer: 第104回日本繁殖生物学会大会
  • Place of Presentation: 盛岡市
  • Year and Date: 2011-09-15
• [Presentation] 単為発生胚由来線維芽細胞を用いたマイクロアレイによる新規インプリント遺伝子の探索 (2011)
  • Author(s): 金田正弘、赤木悟史、渡辺伸也、Tamas Somfai、下司雅也、橋谷田豊、高橋昌志、山中賢一、齋藤公治、永井卓
  • Organizer: 第5回日本エピジェネティクス研究会年会
  • Place of Presentation: 熊本市
  • Year and Date: 2011-05-19
• [Presentation] 単為発生胚由来線維芽細胞を用いたマイクロアレイによる新規インプリント遺伝子の探索 (2011)
  • Author(s): 金田正弘、赤木悟史、渡辺伸也、Tamas Somfai、下司雅也、橋谷田豊、高橋昌志、山中賢一、齋藤公治、永井卓
  • Organizer: 第5回日本エピジェネティクス研究会年会
  • Place of Presentation: KKRホテル熊本(熊本県)
  • Year and Date: 2011-05-19
• [Presentation] Analysis of bovine parthenogenetic embryonic fibroblasts and search for novel imprinted genes in cattle (2010)
  • Author(s): 金田正弘、赤木悟史、渡辺伸也、Somfai Tamas、原口清輝、下司雅也、橋谷田豊、高橋昌志、山中賢一、斎藤公治、森正臣、永井卓
  • Organizer: International Symposium on "Epigenome Network, Development and Reprogramming of Germ Cells"
  • Place of Presentation: 福岡市
  • Year and Date: 2010-11-23
• [Presentation] Analysis of bovine parthenogenetic embryonic fibroblasts and search for novel imprinted genes in cattle (2010)
  • Author(s): 金田正弘, 赤木悟史, 渡邊伸也, Somfai Tamas, 原口清輝, 下司雅也, 橋谷田豊, 高橋昌志, 山中賢一, 斎藤公治, 森正臣, 永井卓
  • Organizer: International Symposium on "Epigenome Network, Development and Reprogramming of Germ Cells"
  • Place of Presentation: 九州大学医学部(福岡県福岡市)
  • Year and Date: 2010-11-23