| Project/Area Number |
22658091
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
AOKI Fugaku 東京大学, 大学院・新領域創成科学研究科, 教授 (20175160)
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|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,620,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | 卵 / 初期胚 / リプログラミング / iPS細胞 / 山中因子 / 受精卵 / 全能性 |
|---|
| Research Abstract |
The expression of the transcripts of Yamanaka factors was examined by RT-PCR, which revealed that all of the factors were detected in the preimplantation embryos. Since the expression levels of Oct3/4 and c-Myc seemed to be high, I focused on these genes in the further experiments. Immunocytochemistry revealed that the nuclear localization of both of OCT3/4 and c-MYC proteins increased after fertilization. When full-grown oocytes were microinjected with Oct3/4 siRNA and then subjected to in vitro fertilization, the fertilized oocytes did not cleave to the 2-cell stage. In these oocytes, the expressions of the genes which are usually expressed after fertilization were reduced.
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