| Project/Area Number |
22658095
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | Hokkaido University |
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Principal Investigator |
INABA Mutsumi 北海道大学, 大学院・獣医学研究科, 教授 (00183179)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Kota 北海道大学, 大学院・獣医学研究科, 准教授 (50283974)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,560,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | 脂質過酸化 / ヒドロキシノネナール / 膜 / プリオン病 / 病態 / 構造変化 |
|---|
| Research Abstract |
Pathogenesis of prion diseases involves transition of PrP^C into PrP^<Sc>. The purpose of this study was to examine if the lipid peroxidation product, 4-hydroxy nonenal(HNE) would modify the structure of PrP^C through formation of PrP^C-HNE adducts and trigger the formation of insoluble PrP^<Sc>. The mass spectrometry analysis showed that PrP^C was accessible to HNE modification when the cells expressing PrP^Cwere exposed to exogenous HNE. However, the content of proteinase K-resistant PrP bearing HNE adducts was negligible, indicating that HNE modification would not be a sole cause for PrP^<Sc> formation.
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