| Project/Area Number |
22658101
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Clinical veterinary science
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
INABA Toshio 大阪府立大学, 生命環境科学研究科, 教授 (00137241)
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| Co-Investigator(Kenkyū-buntansha) |
IMAI Hiroshi 京都大学, 農学研究科, 教授 (10303869)
SHUGIURA Kikuya 大阪府立大学, 生命環境科学研究科, 准教授 (30171143)
HATOYA Shingo 大阪府立大学, 生命環境科学研究科, 助教 (40453138)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,440,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | 獣医学 / トランスレーショナルリサーチ / バイオテクノロジー / 移植・再生医療 / 発生・分化 / iPS細胞 / イヌ / ES細胞 / 骨髄間質細胞 / 脊髄損傷 / 神経細胞 / 遺伝子導入 / 再生医療 |
|---|
| Research Abstract |
The objective of this study was to development of cell therapy using induced pluripotent stem(iPS) cells for dogs with spinal cord injury, and the following results were obtained.(1) We successfully reprogrammed canine embryonic fibroblasts into iPS-like cells using lentiviruses containing 4 transcription factors. Canine iPS-like cell colonies displayed a mouse embryonic stem(ES) cell-like morphology, differentiated to multiple cell types in vitro, and maintained their proliferation and mouse ES-like morphology beyond passage 25.(2) Neurons could be induced from canine iPS-like cells.(3) Bone marrow stromal cell transplantation caused no complications and could have beneficial effects on functional recovery of dogs with spinal cord injury, and further studies are required to apply iPS-like cells for the treatment of these dogs.
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