Development of methods to solubilize odorants into aqueous phase using insect odorant binding proteins and their application to odorant biosensor
| Project/Area Number |
22658109
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied molecular and cellular biology
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
SAKURAI Takeshi 東京大学, 先端科学技術研究センター, 特任助教 (20506761)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
MITSUNO Hidefumi 東京大学, 先端科学技術研究センター, 特任研究員 (60511855)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,560,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,000,000 (Direct Cost: ¥2,000,000)
|
|---|
| Keywords | 嗅覚バイオセンサ / 昆虫 / 匂い結合タンパク質 / 嗅覚受容体 / 生体機能利用 |
|---|
| Research Abstract |
In insects, odorant binding proteins solubilize odorant molecules into the sensillum lymph that bathes olfactory receptor neurons in the antennae. In the present study, as the first step to develop methods to solubilize odorant molecules into aqueous phase, we established a large scale expression system and a simple purification protocol of odorant binding proteins from the silkmoth, Bombyx mori, and the fruit fly, Drosophila melanogaster, by using eXact-tag based single-step affinity column purification.
|
Report
(3 results)
Research Products
(6 results)
