| Project/Area Number |
22658111
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Keio University |
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Principal Investigator |
DOI Nobuhide 慶應義塾大学, 理工学部, 准教授 (50327673)
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| Co-Investigator(Renkei-kenkyūsha) |
YANAGAWA Hiroshi 慶應義塾大学, 理工学部, 訪問教授 (40327672)
HORISAWA Kenichi 慶應義塾大学, 理工学部, 助教 (70424207)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,590,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | プロテオーム / 蛋白質 / 遺伝子 / バイオテクノロジー / 発現制御 |
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| Research Abstract |
Protein microarrays, in which thousands of proteins are spotted on a slide glass, is a powerful proteomic tool for high-throughput parallel analysis of protein interactions. We have developed an on-chip transcription/translation microarray, in which protein synthesis is done on a slide glass by cell-free expression systems and subsequently captured by antibodies immobilized on the slide glass. We further developed on-chip co-expression microarrays that co-express a bait protein and a prey protein in a single spot. This approach has an advantage that a variety of combination of bait and prey proteins can be analyzed immediately on a slide. We used transcription factors Jun and Jun-binding proteins as a model, and successfully detected protein-protein and protein-DNA interactions by the on-chip co-expression microarray.
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