| Project/Area Number |
22659007
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHIMADA Miki 東北大学, 病院, 准教授 (10196488)
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| Co-Investigator(Renkei-kenkyūsha) |
MAEKAWA Masamitsu 東北大学, 病院, 助手 (70572882)
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,090,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | 分析化学 / メタボロミクス / LC/MS / 胆汁酸 / 低エネルギーCID / 多次元マップ化 / 3β-HSD欠損症 / Niemann-Pick病typeC / 抱合型胆汁酸 / 3β-HSD / Niemann-Pick病type C |
|---|
| Research Abstract |
We set up an HPLC separation method for various bile acid conjugates, and characterized each fragmentation of bile acid conjugates by the investigation of product ion patterns on low-energy collision-induced dissociation. Based on their data, we produced focusing methods for bile acid metabolome by conjugated forms using precursor ion scan and neutral loss scan analysis. In addition, we analyzed urine from patients producing metabolic abnormalities of cholesterol, and found to analyze disease-specific bile acid metabolites.
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