| Project/Area Number |
22659015
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KATADA Toshiaki 東京大学, 大学院・薬学系研究科, 教授 (10088859)
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| Co-Investigator(Kenkyū-buntansha) |
KAJIHO Hiroaki 東京大学, 大学院・薬学系研究科, 助教 (70401221)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | シグナル伝達 / G蛋白質 / P-body / mRNA動態 / Gサイクル / ゲノムプロジェクト / Processing body |
|---|
| Research Abstract |
Processing bodies(P-bodies) contain a common set of conserved RNA-processing enzymes, and mRNAs with AU-rich elements(AREs) are delivered to P-bodies for translational silencing. It is unclear how small GTPases are involved in the P-body regulation and the ARE-mRNA metabolism. We found here that overexpression of the RhoA-subfamily GTPases alters the P-body dynamics in HeLa cells. Interestingly, both RhoA activation and glucose depletion inhibit the mRNA accumulation and degradation. These findings indicate that RhoA participates in the stress-induced rearrangement of P-bodies and the release of nucleated ARE-mRNAs for their stabilization.
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