| Project/Area Number |
22659018
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
|
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| Research Institution | University of Shizuoka |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,090,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,400,000 (Direct Cost: ¥1,400,000)
|
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| Keywords | リンパ球ホーミング / L-セレクチン / 高内皮細静脈 / 硫酸化糖鎖 / 抗糖鎖抗体 / 糖鎖 / 免疫学 |
|---|
| Research Abstract |
Anti-carbohydrate monoclonal antibodies(mAbs) are very useful in the functional analysis of complex carbohydrates in vivo. However, such mAbs are difficult to generate, largely because a wide variety of complex carbohydrates is intrinsically expressed in mice and rats and because the antigenicities of glycans are generally poor. In this study, we developed an efficient method for generating anti-carbohydrate mAbs using glycan-synthesizing enzyme-knockout mice in which the glycan structures formed by the missing enzymes should be highly antigenic. As an application of this method, we generated anti-sulfated glycan mAbs using sulfotransferase-deficient mice and found that sulfated N-and sulfated O-glycans function cooperatively in lymphocyte homing and immune surveillance using those mAbs.
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