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A novel strategy for the generation of anti-glycan antibodies and its application

Research Project

Project/Area Number 22659018
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionUniversity of Shizuoka

Principal Investigator

KAWASHIMA Hiroto  静岡県立大学, 薬学部, 准教授 (50260336)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,090,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsリンパ球ホーミング / L-セレクチン / 高内皮細静脈 / 硫酸化糖鎖 / 抗糖鎖抗体 / 糖鎖 / 免疫学
Research Abstract

Anti-carbohydrate monoclonal antibodies(mAbs) are very useful in the functional analysis of complex carbohydrates in vivo. However, such mAbs are difficult to generate, largely because a wide variety of complex carbohydrates is intrinsically expressed in mice and rats and because the antigenicities of glycans are generally poor. In this study, we developed an efficient method for generating anti-carbohydrate mAbs using glycan-synthesizing enzyme-knockout mice in which the glycan structures formed by the missing enzymes should be highly antigenic. As an application of this method, we generated anti-sulfated glycan mAbs using sulfotransferase-deficient mice and found that sulfated N-and sulfated O-glycans function cooperatively in lymphocyte homing and immune surveillance using those mAbs.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (34 results)

All 2012 2011 2010 Other

All Journal Article (10 results) (of which Peer Reviewed: 9 results) Presentation (20 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Essential role of peripheral node addressin in lymphocyte homing to nasal-associated lymphoid tissues and allergic immune responses2011

    • Author(s)
      Ohmichi Y, Hirakawa J, Imai Y, Fukuda M, and Kawashima H.
    • Journal Title

      J. Exp. Med

      Volume: 208 Issue: 5 Pages: 1015-1025

    • DOI

      10.1084/jem.20101786

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Binding of L-selectin to its vascular and extravascular ligands is differentially regulated by pH2011

    • Author(s)
      Hirose M, Matsumura R, Sato K, Murai T, and Kawashima H.
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 414 Issue: 2 Pages: 437-442

    • DOI

      10.1016/j.bbrc.2011.09.123

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel anti-carbohydrate antibodies reveal the cooperative function of sulfated N-and O-glycans in lymphocyte homing2010

    • Author(s)
      Hirakawa J, Tsuboi K, Sato K, Kobayashi M, Watanabe S, Takakura A, Imai Y, Ito Y, Fukuda M, and Kawashima H.
    • Journal Title

      J. Biol. Chem

      Volume: 285 Issue: 52 Pages: 40864-40878

    • DOI

      10.1074/jbc.m110.167296

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Endothelial Heparan Sulfate Controls Chemokine Presentation in Recruitment of Lymphocytes and Dendritic Cells to Lymph Nodes2010

    • Author(s)
      Bao X, Moseman EA, Saito H, Petryanik B, Thiriot A, Hatakeyama S, Ito Y, Kawashima H, Yamaguchi Y, Lowe JB, von Andrian UH and Fukuda M.
    • Journal Title

      Immunity

      Volume: 33 Issue: 5 Pages: 817-829

    • DOI

      10.1016/j.immuni.2010.10.018

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sulfation of colonic mucins by N-acetylglucosamine-6-O-sulfotransferase-2 and its protective function in experimental colitis in mice2010

    • Author(s)
      Tobisawa Y, Imai Y, Fukuda M, and Kawashima H.
    • Journal Title

      J. Biol. Chem

      Volume: 285 Issue: 9 Pages: 6750-6760

    • DOI

      10.1074/jbc.m109.067082

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Two roles of mucin sulfation2010

    • Author(s)
      Kawashima H
    • Journal Title

      Trends Glycosci. Glycotechnol

      Volume: 22 Pages: 211-225

    • NAID

      10027893003

    • URL

      http://www.jstage.jst.go.jp/article/tigg/22/127/22_211/_article

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel anti-carbohydrate antibodies reveal the cooperative function of sulfated N- and O-glycans in lymphocyte homing.2010

    • Author(s)
      Hirakawa J, Tsuboi K, Sato K, Kobayashi M, Watanabe S, Takakura A, Imai Y, Ito Y, Fukuda M, Kawashima H
    • Journal Title

      Journal of Biological Chemistry

      Volume: 285 Pages: 40864-40878

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Endothelial Heparan Sulfate Controls Chemokine Presentation in Recruitment of Lymphocytes and Dendritic Cells to Lymph Nodes.2010

    • Author(s)
      Bao X, Moseman EA, Saito H, Petryanik B, Thiriot A, Hatakeyama S, Ito Y, Kawashima H, Yamaguchi Y, Lowe JB, von Andrian UH, Fukuda M
    • Journal Title

      Immunity

      Volume: 33 Pages: 817-829

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two roles of mucin sulfation.2010

    • Author(s)
      Kawashima H
    • Journal Title

      Trends in Glycoscience and Glycotechnology

      Volume: 22 Pages: 211-225

    • NAID

      10027893003

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of GlcNAc-6-O-sulfotransferases in lymphoid and nonlymphoid tissues.2010

    • Author(s)
      Kawashima H
    • Journal Title

      Methods in Enzymology

      Volume: 479 Pages: 243-256

    • Related Report
      2010 Annual Research Report
  • [Presentation] リンパ球ホーミングにおける高内皮細静脈ヘパラン硫酸の機能解析2012

    • Author(s)
      坪井康一郎、平川城太朗、關愛実子、今井康之、山口祐、福田穣、川島博人
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Essential role of peripheral node addressin in lymphocyte homing to nasal-associated lymphoid tissues and allergic immune responses2012

    • Author(s)
      Ohmichi Y, Hirakawa J, Imai Y, Fukuda M, and Kawashima H
    • Organizer
      Keystone Symposia-Chemokines and Leukocyte Trafficking in Homeostasis and Inflammation
    • Place of Presentation
      Breckenridge, Colorado, USA
    • Year and Date
      2012-01-10
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Novel Anti-carbohydrate Antibodies Reveal the Cooperative Function of Sulfated N-and O-Glycans in Lymphocyte Homing2011

    • Author(s)
      Hirakawa J, Tsuboi K, Sato K, Imai Y, and Kawashima H
    • Organizer
      第40回日本免疫学会
    • Place of Presentation
      千葉
    • Year and Date
      2011-11-29
    • Related Report
      2011 Final Research Report
  • [Presentation] Novel Anti-carbohydrate Antibodies Reveal the Cooperative Function of Sulfated N- and O-Glycans in Lymphocyte Homing2011

    • Author(s)
      Hirakawa J, Tsuboi K, Sato K, Imai Y, Kawashima H
    • Organizer
      第40回日本免疫学会
    • Place of Presentation
      千葉
    • Year and Date
      2011-11-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] 大腸特異的Atg7欠損マウスにおけるデキストラン硫酸誘発性大腸炎の解析2011

    • Author(s)
      坪井康一郎、高倉惇、今井康之、小松雅明、川島博人
    • Organizer
      第84回日本生化学会年会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 大腸特異的Atg7欠損マウスにおけるデキストラン硫酸誘発性大腸炎の増悪化2011

    • Author(s)
      坪井康一郎、高倉惇、今井康之、小松雅明、川島博人
    • Organizer
      第75回日本生化学会中部支部例会
    • Place of Presentation
      静岡
    • Year and Date
      2011-05-28
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Novel Anti-Carbohydrate Antibodies Reveal the Cooperative Function of Sulfated N-and O-Glycans in Lymphocyte Homing2011

    • Author(s)
      Hirakawa J, Tsuboi K, Sato K, Kobayashi M, Watanabe S, Takakura A, Imai Y, Ito Y, Fukuda M, and Kawashima H
    • Organizer
      Keystone Symposia-New Frontiers at the Interface of Immunity and Glycobiology
    • Place of Presentation
      Lake Louise, Alberta, Canada
    • Year and Date
      2011-03-08
    • Related Report
      2011 Final Research Report
  • [Presentation] Essential role of peripheral node addressin in lymphocyte homing to nasal-associated lymphoid tissues and allergic immune responses2011

    • Author(s)
      Ohmichi Y, Hirakawa J, Imai Y, Fukuda M, and Kawashima H
    • Organizer
      Keystone Symposia-New Frontiers at the Interface of Immunity and Glycobiology
    • Place of Presentation
      Lake Louise, Alberta, Canada
    • Year and Date
      2011-03-08
    • Related Report
      2011 Final Research Report
  • [Presentation] Essential role of peripheral node addressin in lymphocyte homing to nasal-associated lymphoid tissues and allergic immune responses.2011

    • Author(s)
      Kawashima H.
    • Organizer
      Keystone Symposia-New Frontiers at the Interface of Immunity and Glycobiology
    • Place of Presentation
      Lake Louise, Alberta, Canada
    • Year and Date
      2011-03-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Novel Anti-Carbohydrate Antibodies Reveal the Cooperative Function of Sulfated N- and O-Glycans in Lymphocyte Homing.2011

    • Author(s)
      Hirakawa J, Tsuboi K, Sato K, Kobayashi M, Watanabe S, Takakura A, Imai Y, Ito Y, Fukuda M, Kawashima H
    • Organizer
      Keystone Symposia-New Frontiers at the Interface of Immunity and Glycobiology
    • Place of Presentation
      Lake Louise, Alberta, Canada
    • Year and Date
      2011-03-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 各種SH3ドメインとN-WASPプロリンリッチ部位の相互作用の相違によりN-WASPのアクチンフィラメント形成と細胞運動の相違が生じる2011

    • Author(s)
      廣瀬まゆみ、川島博人、村井稔幸、川上和之、源利成、岡田雅人、末次志郎、竹縄忠臣、中川敦史
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      北海道
    • Related Report
      2011 Final Research Report
  • [Presentation] Minamoto. Elevation of rat plasma P-selectin in acute lung injury2011

    • Author(s)
      Hirose M, Kawashima H, Murai T, Kawakami K
    • Organizer
      日本分子生物学会・第11回春季シンポジウム、金沢国際がん生物学シンポジウム
    • Place of Presentation
      金沢
    • Related Report
      2011 Final Research Report
  • [Presentation] 各種SH3ドメインとN-WASPプロリンリッチ部位の相互作用の相違によりN-WASPのアクチンフィラメント形成と細胞運動の相違が生じる2011

    • Author(s)
      廣瀬まゆみ、川島博人、村井稔幸、川上和之、源利成、岡田雅人、末次志郎、竹縄忠臣、中川敦史
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      札幌
    • Related Report
      2011 Annual Research Report
  • [Presentation] Elevation of rat plasma P-selectin in acute lung injury2011

    • Author(s)
      Hirose M, Kawashima H, Murai T, Kawakami K, Minamoto
    • Organizer
      日本分子生物学会・第11回春季シンポジウム
    • Place of Presentation
      金沢
    • Related Report
      2011 Annual Research Report
  • [Presentation] B16メラノーマの肺転移に及ぼすケモカイン強制発現の影響2010

    • Author(s)
      加賀正基, 渡部草太, 平川城太朗, 今井康之, 川島博人
    • Organizer
      平成22年度日本薬学会東海支部例会
    • Place of Presentation
      静岡
    • Year and Date
      2010-11-28
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 新しい抗糖鎖抗体作製法の樹立に基づく硫酸化糖鎖のリンパ球ホーミングにおける機能の解明2010

    • Author(s)
      川島博人
    • Organizer
      CREST「代謝調節機構解析に基づく細胞機能制御基盤技術」平成22年度第3回公開シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2010-10-21
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Deficiency of L-selectin ligand biosynthesis by knocking out of GlcNAc6ST-1 and-2 suppresses asthmatic response in murine model of asthma2010

    • Author(s)
      Bao X, Nakayama J, Miller M, Pham A, Kobayashi M, Kawashima H, Broide D, Fukuda M
    • Organizer
      Glycobiology2010
    • Place of Presentation
      USA
    • Related Report
      2011 Final Research Report
  • [Presentation] CRIBドメインとWHドメインを介した特定のN-WASP多量体がアクチン骨格形成におよび細胞運動に寄与している2010

    • Author(s)
      廣瀬まゆみ、川島博人、村井稔幸、竹縄忠臣、末次志郎、中川敦史
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] ケモカインCXCL10の強制発現によるB16メラノーマの肺転移抑制2010

    • Author(s)
      加賀正基、渡部草太、平川城太朗、今井康之、川島博人
    • Organizer
      第83回日本生化学会年会
    • Place of Presentation
      神戸
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Deficiency of L-selectin ligand biosynthesis by knocking out of GlcNAc6ST-1 and -2 suppresses asthmatic response in murine model of asthma.2010

    • Author(s)
      Bao X, Nakayama J, Miller M, Pham A, Kobayashi M, Kawashima H, Broide D, Fukuda M
    • Organizer
      Glycobiology2010
    • Place of Presentation
      St.Pete Beach, FL, USA
    • Related Report
      2010 Annual Research Report
  • [Book] Roles of GlcNAc-6-O-sulfotransferases in lymphoid and nonlymphoid tissues. Methods in Enzymology2010

    • Author(s)
      Kawashima H
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://sys.u-shizuoka-ken.ac.jp/~bisei/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://sys.u-shizuoka-ken.ac.jp/~bisei/

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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