Novel recognition mechanisms to chemical compounds which enhance allergic responses

• Project/Area Number: 22659025
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Environmental pharmacy
• Research Institution: Tohoku University
• Principal Investigator: HIRASAWA Noriyasu 東北大学, 大学院・薬学研究科, 教授 (80181155)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,010,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: TSLP / キシレン / ケラチノサイト / 化学物質認識蛋白質 / アレルギー / アトピー性皮膚炎 / 化学物質認識タンパク質 / MAP kinase

Research Abstract

e found that xylene induces a allergy-inducing cytokine thymic stromal lymphopoietin (TSLP)production. In this study, we prepared xylene-bound affinity gels and identified a candidate which selectively binds to xylene. In addition, in an epithelial cell line, we found that ERK plays an important role in induction of TSLP.

Research Products

• [Journal Article] Hetero-oligomerization between adenosine A1 and thromboxane A2 receptors affects cellular signal transduction on stimulation with high and low concentrations of agonists for both receptors (2012)
  • Author(s): Mizuno, N., Suzuki, T., Hirasawa, N., Nakahata, N.
  • Journal Title: Eur. J. Pharmacol Volume: 677 Issue: 1-3 Pages: 5-14
  • DOI: 10.1016/j.ejphar.2011.12.006
  • Peer Reviewed
• [Journal Article] Induction of thymic stromal lymphopoietin production by xylene and exacerbation of picryl chloride-induced allergic inflammation in mice (2012)
  • Author(s): N.Satou
  • Journal Title: Int Arch Allergy Immunol Volume: 157 Issue: 2 Pages: 194-201
  • DOI: 10.1159/000327545
  • Peer Reviewed
• [Journal Article] Education of Graduate School of Pharmaceutical Sciences in New Generation: Introduction (2011)
  • Author(s): 平澤典保、奥直人
  • Journal Title: YAKUGAKU ZASSHI Volume: 131 Issue: 6 Pages: 931-931
  • DOI: 10.1248/yakushi.131.931
  • NAID: 130000926977
  • ISSN: 0031-6903, 1347-5231
  • Year and Date: 2011-06-01
• [Journal Article] Education for Six-years Graduate School of Pharmaceutical Sciences: A Case of Tohoku University (2011)
  • Author(s): 富岡佳久、平澤典保、永沼章
  • Journal Title: YAKUGAKU ZASSHI Volume: 131 Issue: 6 Pages: 933-938
  • DOI: 10.1248/yakushi.131.933
  • NAID: 130000926978
  • ISSN: 0031-6903, 1347-5231
  • Year and Date: 2011-06-01
• [Journal Article] Enhancement of nickel elution by lipopolysaccharide-indued inflammation (2011)
  • Author(s): Tanaka, R., Goi, Y., Ishihara, K., Ueda, K., Narushima, T., Ohtsu, H., Hiratsuka, M., Hirasawa, N
  • Journal Title: J. Derm. Sci Volume: 62 Pages: 50-57
  • DOI: 10.1016/j.jdermsci.2010.12.006
  • Peer Reviewed
• [Journal Article] Assessment of the release of nickel from biomaterials in vivo and in vitro : enhancement by lipopolysaccharide (2011)
  • Author(s): Tanaka, R., Goi, Y., Ishihara, K., Ueda, K., Narushima, T., Ohtsu, H., Ohuchi, K., Hiratsuka, M., Hirasawa, N.
  • Journal Title: Inflam. Regene Volume: 31 Pages: 302-306
  • NAID: 130004943799
  • URL: http://www.jsir.library.gr.mech.journal/
  • Peer Reviewed
• [Journal Article] Induction of thymic stromal lymphopoietin by chemical compounds in vivo and exacerbation of allergy (2011)
  • Author(s): Sato, N., Ishihara, K., Hiratsuka, M., Hirasawa, N.
  • Journal Title: Inflam. Regene Volume: 31 Pages: 184-188
  • NAID: 130004943785
  • URL: http://www.jsir.library.gr.mech.journal/
  • Peer Reviewed
• [Journal Article] Suppression of intracellular calcium levels and inhibition of degranulation in RBL-2H3 mast cells by the sesquiterpene lactone parthenolide (2011)
  • Author(s): Hong, J., Aoyama, S., Hirasawa, N, Zee, O., Ishihara, K., Hashida, C., Kimura, M., Seyama, T., Ohuchi, K.
  • Journal Title: Planta Medica Volume: 77 Issue: 03 Pages: 252-256
  • DOI: 10.1055/s-0030-1250221
  • Peer Reviewed
• [Journal Article] Novel Single Nucleotide Polymorphism of the CYP2A13 Gene in Japanese Individuals (2011)
  • Author(s): Tamaki, Y., Honda, M., Muroi, Y., Arai, T., Sugimura, H., Matsubara, Y., Kanno, S., Ishikawa, M., Hirasawa, N, Hiratsuka, M.
  • Journal Title: Drug Metabolism and Pharmacokinetics Volume: 26 Issue: 5 Pages: 544-547
  • DOI: 10.2133/dmpk.DMPK-11-SC-033
  • NAID: 130004463219
  • ISSN: 1347-4367, 1880-0920
  • Peer Reviewed
• [Journal Article] Association between Cancer Risk and Drug-metabolizing Enzyme Gene (CYP2A6, CYP2A13, CYP4B1, SULT1A1, GSTM1, and GSTT1) Polymorphisms in Cases of Lung Cancer in Japan (2011)
  • Author(s): Tamaki, Y., Arai, T., Sugimura, H., Sasaki, T., Honda, M., Muroi, Y., Matsubara, Y., Kanno, S., Ishikawa, M., Hirasawa, N., Hiratsuka, M.
  • Journal Title: Drug Metabolism and Pharmacokinetics Volume: 26 Issue: 5 Pages: 516-522
  • DOI: 10.2133/dmpk.DMPK-11-RG-046
  • NAID: 130004463215
  • ISSN: 1347-4367, 1880-0920
  • Peer Reviewed
• [Journal Article] Functional characterization of CYP2B6 allelic variants in demethylation of anti-malarial artemethe (2011)
  • Author(s): Honda, M., Muroi, Y., Tamaki, Y., Saigusa, D., Suzuki, N., Tomioka, Y., Matsubara Y., Oda, A., Hirasawa, N., Hiratsuka, M.
  • Journal Title: Drug Metab. Dispos Volume: 39 Issue: 10 Pages: 1860-1865
  • DOI: 10.1124/dmd.111.040352
  • Peer Reviewed
• [Journal Article] ステロイドの作用機序と耐性化メカニズム (2011)
  • Author(s): 平澤典保
  • Journal Title: アレルギーの臨床(The Allergy in Practice) Volume: 31 Pages: 1137-1141
• [Journal Article] キシレン、トリメチルベンゼンによる表皮からのTSLP産生誘導 (2011)
  • Author(s): 平澤典保
  • Journal Title: 臨床と免疫・アレルギー科 Volume: 56 Pages: 478-484
• [Journal Article] 抗炎症薬 (2011)
  • Author(s): 平澤典保、Hong JangJa、木邑道夫、大内和雄
  • Journal Title: OHNS Volume: 27 Pages: 1782-1786
• [Journal Article] TSLP (2011)
  • Author(s): 平澤典保、Hong JangJa、木邑道夫、大内和雄
  • Journal Title: 炎症と免疫 Volume: 19 Pages: 437-440
• [Journal Article] ステロイド薬の基礎 (2011)
  • Author(s): 平澤典保
  • Journal Title: アレルギー Volume: 60 Pages: 193-198
  • NAID: 110008460516
  • URL: http://jja.jsaweb.mech.2011/060020193e.html
• [Journal Article] Induction of Thymic stromal lymphopoietin production by xylene and exacerbation of picryl chloride-induced allergic inflammation in mice (2011)
  • Author(s): Satou, N., Iwatate, Y., Leonard, W.J., Hirasawa, N.
  • Journal Title: Int.Arch.Allergy Immunol. Volume: 157 Pages: 194-201
  • Peer Reviewed
• [Journal Article] Enhancement of nickel elution by lipopolysaccharide-indued inflammation (2011)
  • Author(s): Tanaka, R., Goi, Y., Ishihara, K., Ueda, K., Narushima, T., Ohtsu, H., Hiratsuka, M., Hirasawa, N.
  • Journal Title: J.Derm.Sci. Volume: 62 Pages: 50-57
  • Peer Reviewed
• [Journal Article] Induction of thymic stromal lymphopoietin by chemical compounds in vivo and exacerbation of allergy (2011)
  • Author(s): Sato, N., Ishihara, K., Hiratsuka, M., Hirasawa, N.
  • Journal Title: Inflam.Regene. Volume: 31 Pages: 184-188
  • NAID: 130004943785
  • Peer Reviewed
• [Journal Article] Assessment of the release of nickel from biomaterials in vivo and in vitro : enhancement by lipopolysaccharide (2011)
  • Author(s): Tanaka, R., Goi, Y., Ishihara, K., Ueda, K., Narushima, T., Ohtsu, H., Ohuchi, K., Hiratsuka, M., Hirasawa, N.
  • Journal Title: Inflam.Regene. Volume: 31 Pages: 302-306
  • NAID: 130004943799
  • Peer Reviewed
• [Journal Article] Induction of Thymic stromal lymphopoietin production by xylene and exacer bation of picryl chloride-induced allergic inflammation in mice (2011)
  • Author(s): Satou, N.
  • Journal Title: Int.Arch.Allergy Immunol. Volume: (掲載確定)(未定)(印刷中)
  • Peer Reviewed
• [Journal Article] TSLP (2011)
  • Author(s): 平澤典保
  • Journal Title: 炎症と免疫 Volume: (掲載確定)(未定)(印刷中)
• [Journal Article] Salicylate restores transport function and anion exchanger activity of missense pendrin mutations (2010)
  • Author(s): Ishihara, K., Okuyama, S., Kumano, S., Iida, K., Hamana, H., Murakoshi, M., Kobayashi, T., Usami, S., Ikeda, K, Haga, Y, , Tsumoto, K., Nakamura, H., Hirasawa, N., Wada, H.
  • Journal Title: Hearing Research Volume: 270 Issue: 1-2 Pages: 110-118
  • DOI: 10.1016/j.heares.2010.08.015
  • Peer Reviewed
• [Journal Article] Kinetics of 6-Thioxanthine Metabolism by Allelic Variants of Xanthine Oxidase (2010)
  • Author(s): Kudo, M., Sasaki, T., Ishikawa, M., Hirasawa, N., Hiratsuka, M.
  • Journal Title: Drug Metabolism and Pharmacokinetics Volume: 25 Issue: 4 Pages: 361-366
  • DOI: 10.2133/dmpk.DMPK-10-RG-029
  • NAID: 10027583274
  • ISSN: 1347-4367, 1880-0920
  • Peer Reviewed
• [Journal Article] Functional Characterization of Genetic Polymorphisms Identified in the Promoter Region of the Xanthine Oxidase Gene (2010)
  • Author(s): Kudo, M., Sasaki, S., Ishikawa, M., Hirasawa, N., Hiratsuka., M.
  • Journal Title: Drug Metabolism and Pharmacokinetics Volume: 25 Issue: 6 Pages: 599-604
  • DOI: 10.2133/dmpk.DMPK-10-NT-054
  • NAID: 10027743970
  • ISSN: 1347-4367, 1880-0920
  • Peer Reviewed
• [Journal Article] Functional characterization of 26 CYP2B6 allelic variants (CYP2B6. 2-CYP2B6. 28, except CYP2B6. 22) (2010)
  • Author(s): Watanabe, T., Sakuyama, K., Sasaki, T., Ishii, Y., Ishikawa, M., Hirasawa, N.
  • Journal Title: Hiratsuka, M. Pharmacogenet. Genom Volume: 20 Issue: 7 Pages: 459-462
  • DOI: 10.1097/fpc.0b013e32833bba0e
  • Peer Reviewed
• [Journal Article] Involvement of prostaglandins and histamine in Nickel wire-induced acute inflammation in mice (2010)
  • Author(s): Hirasawa, N., Goi, Y., Tanaka, R., Ishihara, K., Ohtsu, H., Ohuchi, K.
  • Journal Title: J. Biomedical Materials Research Part Volume: 93A Issue: 4 Pages: 1306-1311
  • DOI: 10.1002/jbm.a.32628
  • Peer Reviewed
• [Presentation] 中鎖脂肪酸によるTSLP産生誘導とアレルギー増悪化作用の解析 (2012)
  • Author(s): 山下紗緒里、佐藤望未、平澤典保
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
• [Presentation] マウスにおける金属からのニッケルイオン溶出過程とその制御機構の解析 (2012)
  • Author(s): 高野貴幸、青島祐佑、田中里奈、浅川三喜、上田恭介、大津浩、成島尚之、平澤典保
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
• [Presentation] 前脂肪細胞におけるHDAC阻害薬によるIL-6産生増加とその機序の検討 (2012)
  • Author(s): 佐藤大樹、平澤典保
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
• [Presentation] 中鎖脂肪酸によるTSLP産生誘導ととアレルギー増悪化作用の解析 (2012)
  • Author(s): 山下紗緒里、佐藤望未、平澤典保
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
• [Presentation] 化学物質によるTSLP産生とアレルギー増悪化 (2011)
  • Author(s): 平澤典保
  • Organizer: 平成23年度日本薬学会東北支部講演会第33回東北薬学セミナー
  • Place of Presentation: 仙台
  • Year and Date: 2011-12-03
• [Presentation] 化学物質によるTSLP産生とアレルギー増悪化 (2011)
  • Author(s): 平澤典保
  • Organizer: 平成23年度日本薬学会東北支部講演会第33回東北薬学セミナー
  • Place of Presentation: 仙台(招待講演)
  • Year and Date: 2011-12-03
• [Presentation] 空気嚢型炎症モデルにおけるTSLP産生制御機構の解析 (2011)
  • Author(s): 瀬川良佑、平澤典保
  • Organizer: 第50回記念日本薬学会東北支部大会
  • Place of Presentation: 仙台
  • Year and Date: 2011-10-30
• [Presentation] 前脂肪細胞株OP9におけるTLR4刺激によるIL-6産生:HDAC阻害薬の効果 (2011)
  • Author(s): 佐藤大樹、平澤典保
  • Organizer: 第50回記念日本薬学会東北支部大会
  • Place of Presentation: 仙台
  • Year and Date: 2011-10-30
• [Presentation] 中鎖脂肪酸によるTSLP産生誘導作用の解析 (2011)
  • Author(s): 山下紗緒里
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-30
• [Presentation] 抗マラリア薬アーテメーター代謝におけるCYP2B6遺伝子多型バリアントの機能解析 (2011)
  • Author(s): 平塚真弘、本田雅志、三枝大輔、鈴木直人、富岡佳久、平澤典保
  • Organizer: 第32回日本臨床薬理学会年会
  • Place of Presentation: 浜松
• [Presentation] Oxidative metabolism of n-desmetyltamoxifen to endoxifen by 50 Cyp2D6 allelic variants (2011)
  • Author(s): Masahiro Hiratsuka, Yuka Muroi, Kanako Sakuyama, Yui Niinuma, Takahiro Saito, Masamitsu Takahashi and Noriyasu Hirasawa
  • Organizer: 日本薬物動態学会第26回年会
  • Place of Presentation: 広島
• [Presentation] 金属アレルギーの新たなアプローチ (2011)
  • Author(s): 平澤典保
  • Organizer: 第15回日本ヒスタミン学会シンポジウム「炎症・アレルギーを巡る最近のトピックス」
  • Place of Presentation: 盛岡
• [Presentation] 肺がん発症リスクと薬物代謝酵素遺伝子多型との関連 (2011)
  • Author(s): 平塚真弘、田巻佑一朗、新井冨生、椙村春彦、平澤典保
  • Organizer: 第21回日本医療薬学会年会
  • Place of Presentation: 神戸
• [Presentation] マウスアトピー性皮膚炎モデルにおけるヒスタミンH1およびH4受容体拮抗薬の併用による治療効果 (2011)
  • Author(s): 大澤雄亮、平澤典保
  • Organizer: 第15回日本ヒスタミン学会
  • Place of Presentation: 盛岡
• [Presentation] 東北大学病院における腎機能低下患者の割合と腎排泄型薬剤の使用状況調査 (2011)
  • Author(s): 高橋亜希、平塚真弘、本田雅志、田巻佑一朗、室井祐佳、新沼優衣、眞野成康、平澤典保
  • Organizer: 第5回日本腎と薬剤研究会学術大会
  • Place of Presentation: 北九州
• [Presentation] An exacerbated allergic dermatitis model in mice and participation of histamine (2010)
  • Author(s): Hirasawa, N.
  • Organizer: 2010 International Symposium on New Drug Development
  • Place of Presentation: Cheondju, Korea
  • Year and Date: 2010-11-17
• [Presentation] 薬物代謝酵素遺伝子多型と肺がんリスクとの関連 (2010)
  • Author(s): 田巻佑一郎、新井富生、椙村春彦、佐々木崇光、平澤典保、平塚真弘
  • Organizer: 第49回日本薬学会東北支部大会
  • Place of Presentation: 郡山
  • Year and Date: 2010-10-24
• [Presentation] マスト細胞とTSLP (2010)
  • Author(s): 平澤典保
  • Organizer: シンポジウム「秋の夜長とマストセル」第14回日本ヒスタミン学会
  • Place of Presentation: 川崎
• [Presentation] 金属系材料のマウス皮下における腐食挙動 (2010)
  • Author(s): 青島有佑、上田恭介、大津浩、成島尚之、小笠原康悦、平澤典保
  • Organizer: 日本金属学会第147回大会
  • Place of Presentation: 札幌
• [Presentation] ステロイド薬の基礎シンポジウム「アレルギー疾患の薬物治療に役立つ基礎薬理学」 (2010)
  • Author(s): 平澤典保
  • Organizer: 第22回日本アレルギー学会春季臨床大会
  • Place of Presentation: 京都
• [Presentation] 増悪化アレルギー性皮膚炎症におけるヒスタミンの役割シンポジウム「アレルギー疾患におけるヒスタミン研究の新展開」 (2010)
  • Author(s): 平澤典保
  • Organizer: 第130年会日本薬学会
  • Place of Presentation: 岡山
• [Presentation] 生体内における金属からのニッケル溶出の簡易測定法の確立と溶出機序の解析 (2009)
  • Author(s): 田中里奈、五井嘉明、石原研治、上田恭介、成島尚之、大津浩、大内和雄、平澤典保
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
• [Remarks]
  • URL: http://www.pharm.tohoku/ac/jp/-seikatsu/mysite1/index.html
• [Remarks]
  • URL: http://www.pharm.tohoku.ac.jp/~seikatsu/mysitel/index.html
• [Patent(Industrial Property Rights)] TSLPを恒常的に高発現する細胞、および前記細胞を利用したTSLP調節剤のスクリーニング方法 (2011)
  • Inventor(s): 平澤典保、秀道広
  • Industrial Property Rights Holder: 東北大学
  • Filing Date: 2011-06-13
  • Overseas
• [Patent(Industrial Property Rights)] 特許(発明)TSLPを恒常的に高発現する細胞、および前記細胞を利用したTSLP調節剤のスクリーニング方法 (2011)
  • Inventor(s): 平澤典保, 秀道広
  • Industrial Property Rights Holder: 東北大学
  • Filing Date: 2011-06-13
  • Overseas