| Project/Area Number |
22659028
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Medical pharmacy
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| Research Institution | Tohoku University |
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Principal Investigator |
YAMAZOE Yasushi 東北大学, 大学院・薬学研究科, 教授 (00112699)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHINARI Kouichi 東北大学, 大学院・薬学研究科, 准教授 (60343399)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,060,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | CYP3A4 / CYP3A11 / コレステロール / オキシステロール / 脂質代謝 / アデノウイルス / 転写調節 / 核内受容体 / 肝臓 |
|---|
| Research Abstract |
In this study, we have obtained following findings : 1)the expressions of mouse Cyp3a11 and human CYP3A4 in hepatocytes are modulated by cellular cholesterol levels, 2)SREBP-2 activated by reduced cellular cholesterol levels down-regulates the expressions of CYP3A genes, 3)LXRα, which is activated by cholesterol metabolites oxysterols, transactivates the expression of CYP3A genes. Moreover, we demonstrated that the adenoviral overexpression of CYP3A4 changed the expression profile of genes associated with lipid metabolism in cultured mouse hepatocytes.
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