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Visualizing gene expression in response to environmental stimuli :real-time bioluminescence imaging in freely moving mice

Research Project

Project/Area Number 22659047
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionHokkaido University

Principal Investigator

HONMA Sato  北海道大学, 大学院・医学研究科, 特任教授 (20142713)

Co-Investigator(Renkei-kenkyūsha) HAMADA Toshiyuki  北海道大学, 大学院・医学研究科, 特任講師 (20360208)
Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsイメージング / 時計遺伝子 / 生体リズム / 生物発光 / 転写調節 / 生物リズム
Research Abstract

We aimed to develop a real-time monitoring system for reporter gene expression from the body surface of freely moving mice which can respond to various environmental stimuli. Using 2 CCD cameras, we performed stereoimaging to identify the region in a space and calibrated the luminescence in real-time. We tracked the region of interest by using 3 markers surrounding the region. By using this system and programs, we succeeded in demonstrating Per1-luc and Bmal1-luc expression from the olfactory bulb, cerebral cortex and skin simultaneously from freely moving mice at real time for more than 3 consecutive days. The system we developed enables us to monitor gene expression not only in single animal but to various social signals.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (60 results)

All 2013 2012 2011 2010 Other

All Journal Article (17 results) (of which Peer Reviewed: 15 results) Presentation (33 results) (of which Invited: 9 results) Book (7 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Daily exposure to cold phase-shifts circadian clock of neonatal rat in vivo2013

    • Author(s)
      Yoshikawa T, Matsuno A, Yamanaka Y, Nishide S, Honma S and Honma K
    • Journal Title

      Eur.J.Neurosci

      Volume: 37 Issue: 3 Pages: 491-497

    • DOI

      10.1111/ejn.12052

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential responses of circadian Per2 expression rhythms in discrete brain areas to daily injection of methamphetamine and restricted feeding in rats.2013

    • Author(s)
      Natsubori A. et al.
    • Journal Title

      Eur.J.Neurosci.

      Volume: 37 Issue: 2 Pages: 251-258

    • DOI

      10.1111/ejn.12034

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Topological specificity and hierarchical network of the circadian calcium rhythm in the suprachiasmatic nucleus2012

    • Author(s)
      Enoki R, Kuroda S, Ono C, Hasan MT, Honma S, Ueda T and Honma K
    • Journal Title

      Proc.Natl. Acad.Sci USA

      Volume: 109 Issue: 52 Pages: 21498-21503

    • DOI

      10.1073/pnas.1214415110

    • NAID

      120005295647

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Suprachiasmatic nucleus: cellular clocks and networks2012

    • Author(s)
      Honma S, Ono D, Suzuki Y, Inagaki N, Yoshikawa T, Nakamura W and Honma K
    • Journal Title

      In Progress in Brain Res

      Volume: 199 Pages: 129-141

    • DOI

      10.1016/b978-0-444-59427-3.00029-0

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Single-cell resolution fluorescence imaging of circadian rhythms detected with a Nipkow spinning disk confocal system.2012

    • Author(s)
      Enoki R. et al.
    • Journal Title

      J Neurosci Methods

      Volume: 202 Issue: 1 Pages: 72-79

    • DOI

      10.1016/j.jneumeth.2012.03.004

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] JNK regulates the photic response of the mammalian circadian clock2012

    • Author(s)
      Yoshitane, H., Honma, S., Fukada, Y., 他11名、6番目
    • Journal Title

      EMBO Rep.

      Volume: (印刷中) Issue: 5 Pages: 455-461

    • DOI

      10.1038/embor.2012.37

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synchronized ATP oscillations have a critical role in prechondrogenic condensation during chondrogenesis2012

    • Author(s)
      Kwon HJ, Ohmiya Y, Honma K-I, Hnma S, nagai T, Saito K, Yasuda K
    • Journal Title

      Cell Death and Disease

      Volume: 3 Issue: 3 Pages: e278-e278

    • DOI

      10.1038/cddis.2012.20

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] De novo synthesis of PERIOD initiates circadian oscillation in cultured mouse suprachiasmatic nucleus after prolonged inhibition of protein synthesis by cycloheximide2012

    • Author(s)
      Nishide, S.Y., Ono, D., Yamada, Y., Honma, S., Honma, K.
    • Journal Title

      Eur.J.Neurosci.

      Volume: 35 Issue: 2 Pages: 291-299

    • DOI

      10.1111/j.1460-9568.2011.07952.x

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synchronized ATP oscillations have a critical role in prechondrogenic condensation during chondrogenesis.2012

    • Author(s)
      Kwon HJ, et al.
    • Journal Title

      Cell Death and Disease

      Volume: 3

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Suprachiasmatic nucleus: cellular clocks and networks.2012

    • Author(s)
      Honma S, et al.
    • Journal Title

      Prog Brain Res

      Volume: 199 Pages: 129-141

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Light responsiveness of clock genes, Per1 and Per2, in the olfactory bulb of mice2011

    • Author(s)
      Hamada T, Homna S, Honma K.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 409 Issue: 4 Pages: 727-731

    • DOI

      10.1016/j.bbrc.2011.05.076

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multichannel perfusion culture bioluminescence reporter system for long-term detection in living cells2010

    • Author(s)
      Watanabe T, Enomoto T, Takahashi M, Honma S, Honma K, Ohmiya Y
    • Journal Title

      Anal. Biochem

      Volume: 402 Issue: 1 Pages: 107-109

    • DOI

      10.1016/j.ab.2010.03.014

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood2010

    • Author(s)
      Ebisawa T
    • Journal Title

      Neurosci Res

      Volume: 66 Pages: 223-227

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multichannel perfusion culture bioluminescence reporter system for long-term detection in living cells2010

    • Author(s)
      Watanabe T
    • Journal Title

      Anal.Biochem.

      Volume: 402 Pages: 107-109

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Loss of circadian rhythm and light-induced suppression of pineal melatonin levels in Cry1 and Cry2 double-deficient mice2010

    • Author(s)
      Yamanaka Y
    • Journal Title

      Genes to Cells

      Volume: 15 Pages: 1063-1071

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 生物時計のイメージング2010

    • Author(s)
      本間さと
    • Journal Title

      自律神経

      Volume: 47 Pages: 102-106

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 体のリズムを統合する脳の時計機能2010

    • Author(s)
      本間さと
    • Journal Title

      ビオフィリア

      Volume: 6 Pages: 13-19

    • Related Report
      2010 Annual Research Report
  • [Presentation] 光が告げる生物時計の振動2013

    • Author(s)
      本間さと
    • Organizer
      先端的光イメージング拠点形成プログラムクロージングシンポジウム
    • Place of Presentation
      北海道大学医学研究科フラテ会館、札幌
    • Year and Date
      2013-03-18
    • Related Report
      2011 Final Research Report
  • [Presentation] 動体追跡技術による全身の時計遺伝子発現定量解析と行動解析2013

    • Author(s)
      浜田俊幸、石川正純、KennethSutherland,宮本直樹、白土博樹、本間さと、本間研一
    • Organizer
      第155回日本獣医学会学術大会
    • Place of Presentation
      東京大学駒場キャンパス、東京
    • Related Report
      2011 Final Research Report
  • [Presentation] 動体追跡技術による全身の時計遺伝子発現定量解析と行動解析2013

    • Author(s)
      浜田俊幸
    • Organizer
      第155回日本獣医学会学術大会
    • Place of Presentation
      東京大学駒場キャンパス(東京都目黒区)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Multi-oscillator System of Mammalian Circadian Clock. Symposium "Molecular and cellular mechanisms of circadian clock and sleep2012

    • Author(s)
      Honma S. Natsubori A, Honma K
    • Organizer
      Asian Society for Sleep Research
    • Place of Presentation
      Taipei International Convention Center, Taiwan
    • Related Report
      2011 Final Research Report
  • [Presentation] Monitoring the circadian clock'stick; multiple hands for multiple functions2012

    • Author(s)
      Honma S
    • Organizer
      4th International Symposium on PhotonicBioimaging
    • Place of Presentation
      Hokkaido UniversityConference Center, Sapporo
    • Related Report
      2011 Final Research Report
  • [Presentation] 時計遺伝子と生物時計の発達2012

    • Author(s)
      本間さと
    • Organizer
      日本小児神経学会第42回小児神経学セ ミナー
    • Place of Presentation
      湘南国民村センター、葉山
    • Related Report
      2011 Final Research Report
  • [Presentation] 覚醒マウスにおける時計遺伝子発現の4Dイメージング2012

    • Author(s)
      浜田俊幸、石川正純、KennethSutherland,宮本直樹、白土博樹、本間さと、本間研一
    • Organizer
      第2回睡眠研究会.
    • Place of Presentation
      名古屋大学
    • Related Report
      2011 Final Research Report
  • [Presentation] Signal integration of circadian neuronal networks2012

    • Author(s)
      Honma S
    • Organizer
      Society for Research on Biological Rhythms Biannual meeting 2012
    • Place of Presentation
      Sandestin Golf and Beach Resort, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Suprachiasmatic nucleus output couplings. Suprachiasmatic Structure Workshop2012

    • Author(s)
      Honma S
    • Organizer
      Society for Research on Biological Rhythms Biannual meeting 2012
    • Place of Presentation
      Sandestin Golf and Beach Resort, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] 生物時計の発達と睡眠覚醒リズム:時間生物学の小児科学への応用2012

    • Author(s)
      本間さと
    • Organizer
      日本小児神経学会
    • Place of Presentation
      札幌
    • Related Report
      2011 Final Research Report
  • [Presentation] Continuous monitoring of circadian clock' s tick in the suprachiasmatic neurons2012

    • Author(s)
      Honma S
    • Organizer
      International Syposium on Frontiers in Sleep and Biological Rhythms' Research
    • Place of Presentation
      Dokuz Eylul University Izmir, Turkey
    • Related Report
      2011 Final Research Report
  • [Presentation] Mammalian circadian clocks detecting morning light and evening lights2012

    • Author(s)
      Honma S, Yoshikawa T and Honma K
    • Organizer
      6th World congress of the World Sleep Federation
    • Place of Presentation
      Kyoto International Conference Center, Kyoto
    • Related Report
      2011 Final Research Report
  • [Presentation] Suprachiasmatic nucleus: cellular clocks and networks2012

    • Author(s)
      Honma S
    • Organizer
      12th Congress of European Society for Biological Rhythm
    • Place of Presentation
      Oxford, UK
    • Related Report
      2011 Final Research Report
  • [Presentation] Continuous monitoring of circadian clock’ s tick in the suprachiasmatic neurons.2012

    • Author(s)
      Sato Honma
    • Organizer
      International Syposium on Frontiers in Sleep and Biological Rhythms’ Research
    • Place of Presentation
      Dokuz Eylul University Izmir (Turkey)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] 生物時計の発達と睡眠覚醒リズム:時間生物学の小児科学への応用2012

    • Author(s)
      本間さと
    • Organizer
      日本小児神経学会
    • Place of Presentation
      札幌ロイトンホテル(北海道札幌市)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Signal integration of circadian neuronal networks.2012

    • Author(s)
      Sato Honma
    • Organizer
      Society for Research on Biological Rhythms Biannual meeting
    • Place of Presentation
      Sandestin Resort(Destin, FL,USA)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Suprachiasmatic nucleus output couplings.2012

    • Author(s)
      Sato Honma
    • Organizer
      Society for Research on Biological Rhythms Biannual meeting
    • Place of Presentation
      Sandestin Resort(Destin, FL,USA)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] 覚醒マウスにおける時計遺伝子発現の4Dイメージング2012

    • Author(s)
      浜田俊幸
    • Organizer
      第2回睡眠研究会
    • Place of Presentation
      名古屋大学シンポジオン(愛知県名古屋市)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] 中枢時計の発達と時計遺伝子機能2012

    • Author(s)
      小野大輔
    • Organizer
      第19回日本時間生物学会学術大会
    • Place of Presentation
      北海道大学学術交流会館(北海道札幌市)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Monitoring the circadian clock’s tick; multiple hands for multiple functions2012

    • Author(s)
      Sato Honma
    • Organizer
      4th International Symposium on Photonic Bioimaging
    • Place of Presentation
      Hokkaido University Conference Center(Sapporo, Hokkaidou)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] 睡眠リズム発生のメカニズム2011

    • Author(s)
      本間さと
    • Organizer
      日本学術会議「脳と意識」「神経科学」「脳とこころ」分科会合同シンポジウム「脳と睡眠」
    • Place of Presentation
      東京(日本学術会議)
    • Year and Date
      2011-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Cellular rhythms and oscillatory networks in the suprachiasmatic nucleus. Workshop: Emergence of circadian rhythms from the central pacemaker and beyond2011

    • Author(s)
      Honma S
    • Organizer
      Institute of Theoretical Biology
    • Place of Presentation
      Humboldt University, Germany
    • Year and Date
      2011-08-15
    • Related Report
      2011 Final Research Report
  • [Presentation] single neuronal rhythms in the SCN. Symposium: Molecular and network properties of the suprachiasmatic nucleus2011

    • Author(s)
      Honma S. Network vs
    • Organizer
      the 3rd World Congress of Chronobiology
    • Place of Presentation
      Cultural Center of BUAP,Puebra, Mexico
    • Related Report
      2011 Final Research Report
  • [Presentation] 光イメージングによる挑戦;長期・マルチ・in vivo計測2010

    • Author(s)
      本間さと
    • Organizer
      第5回北海道大学大学院医学研究科連携研究センターシンポジウム
    • Year and Date
      2010-10-01
    • Related Report
      2011 Final Research Report
  • [Presentation] Animal model of human sleep-wake rhythm2010

    • Author(s)
      Honma S. and Honma K
    • Organizer
      The 11thCongress of Turkish Sleep Society
    • Place of Presentation
      Maritim Pine Beach Resort, Antalya, Turkey
    • Related Report
      2011 Final Research Report
  • [Presentation] Assessing multi-oscillator mammalian clock using mice with clock gene mutation. Lorenz Center Workshop2010

    • Author(s)
      Honma S
    • Organizer
      Assembling multi-cellular Circadian Pacemaker
    • Place of Presentation
      Lorenz Center, Leiden, The Netherlands
    • Related Report
      2011 Final Research Report
  • [Presentation] 行動リズムと時計遺伝子機能2010

    • Author(s)
      本間さと、小野大輔、本間研一
    • Organizer
      第87回日本生理学会大会
    • Place of Presentation
      盛岡市民文化ホール、いわて県民情報交流センター(盛岡市)
    • Related Report
      2011 Final Research Report
  • [Presentation] Assessing multi-oscillator mammalian clock using mice with clock gene mutation2010

    • Author(s)
      Honma S.
    • Organizer
      Lorenz Center Workshop
    • Place of Presentation
      Leiden University, Leiden, Netherlands(招待講演)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Animal model of human sleep-wake rhythm2010

    • Author(s)
      Honma S
    • Organizer
      The 11th Congress of Turkish Sleep Society
    • Place of Presentation
      Maritim Pine Beach Hotel, Antalya, Turkey(招待講演)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 行動リズムと時計遺伝子機能2010

    • Author(s)
      本間さと
    • Organizer
      第87回日本生理学会大会
    • Place of Presentation
      盛岡市民文化ホール(招待講演)
    • Related Report
      2010 Annual Research Report
  • [Presentation] In vivo bioluminescence reporter gene imaging in the mouse brain2010

    • Author(s)
      Hamada T
    • Organizer
      8th International Symposium for Future Drug Discovery and Medical Care
    • Place of Presentation
      北海道大学、札幌市
    • Related Report
      2010 Annual Research Report
  • [Presentation] In vivo bioluminescence reporter gene imaging of the olfactory bulb in the mouse brain2010

    • Author(s)
      Hamada T
    • Organizer
      2010 World Molecular Imaging Congress
    • Place of Presentation
      Kyoto Internatonal Conference Center, Kyoto
    • Related Report
      2010 Annual Research Report
  • [Presentation] in vivo4Dイメージングによる覚醒マウス時計遺伝子発現定量解析

    • Author(s)
      浜田俊幸
    • Organizer
      第19回日本時間生物学会学術大会
    • Place of Presentation
      北海道大学学術交流会館 (北海道札幌市)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Book] In "Advances in Cognitive Neurodynamics (III)"2013

    • Author(s)
      Honma S, Ono D and Honma k
    • Publisher
      Springer, Tokyo (in press)
    • Related Report
      2011 Final Research Report
  • [Book] 時間生物学2012

    • Author(s)
      本間さと
    • Publisher
      化学同人
    • Related Report
      2011 Final Research Report
  • [Book] DOJIN SCIENCE SERIES 02 時間生物学2012

    • Author(s)
      本間 さと
    • Publisher
      科学同人
    • Related Report
      2011 Annual Research Report
  • [Book] 嗅球の体内時計と嗅覚刺激の作用、体内時計の科学と産業応用2011

    • Author(s)
      浜田俊幸、本間さと、本間研一
    • Publisher
      シーエムシー
    • Related Report
      2011 Final Research Report
  • [Book] Molecular Imaging for Integrated Medical Therapy and Drug Development2010

    • Author(s)
      Honma S, Yoshikawa T, Nishide S, Ono D and Honma K
    • Publisher
      Springer, Tokyo
    • Related Report
      2011 Final Research Report
  • [Book] 環境応答-概日リズムからだと光の事典2010

    • Author(s)
      本間さと
    • Publisher
      朝倉書店
    • Related Report
      2011 Final Research Report
  • [Book] からだと光の事典2010

    • Author(s)
      本間さと
    • Total Pages
      421
    • Publisher
      朝倉書店
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.hucc.hokudai.ac.jp/~a10071/

    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] ターゲット位置追跡装置およびターゲット発光検出装置2010

    • Inventor(s)
      石川正純、浜田俊幸、本間研一、本間さと、ケネス・リー・サザーランド
    • Industrial Property Rights Holder
      北海道大学
    • Industrial Property Number
      2010-245624
    • Filing Date
      2010-11-01
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] ターゲット位置追跡装置およびターゲット発光検出装置2010

    • Inventor(s)
      幸, 本間研一, 本間さと, K.サザーランド
    • Industrial Property Rights Holder
      北海道大学
    • Industrial Property Number
      2010-245624
    • Filing Date
      2010-11-01
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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