Visualizing gene expression in response to environmental stimuli :real-time bioluminescence imaging in freely moving mice
| Project/Area Number |
22659047
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Hokkaido University |
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Principal Investigator |
HONMA Sato 北海道大学, 大学院・医学研究科, 特任教授 (20142713)
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| Co-Investigator(Renkei-kenkyūsha) |
HAMADA Toshiyuki 北海道大学, 大学院・医学研究科, 特任講師 (20360208)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | イメージング / 時計遺伝子 / 生体リズム / 生物発光 / 転写調節 / 生物リズム |
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| Research Abstract |
We aimed to develop a real-time monitoring system for reporter gene expression from the body surface of freely moving mice which can respond to various environmental stimuli. Using 2 CCD cameras, we performed stereoimaging to identify the region in a space and calibrated the luminescence in real-time. We tracked the region of interest by using 3 markers surrounding the region. By using this system and programs, we succeeded in demonstrating Per1-luc and Bmal1-luc expression from the olfactory bulb, cerebral cortex and skin simultaneously from freely moving mice at real time for more than 3 consecutive days. The system we developed enables us to monitor gene expression not only in single animal but to various social signals.
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Report
(3 results)
Research Products
(58 results)
